Studying the influence of 2-((4-R-3- (morfolinomethylen)-4H-1,2,4-triazole-5-yl) thio)acetic acid salts on growth and progress of corn sprouts

Topicality. Agricultural industry urgently requires new and effective growth stimulating remedies. 1,2,4-triazole derivatives have had exhibited themselves as active fungicides and growth stimulators and the salts of 2-(4-R-3-R11,2,4-triazole-5-yltio)acetic acid are quite promising in this aspect. Aim. To determine the impact on quality indicators of Galatea hybrid corn sprouts germination after the processing with solutions of 2-((4-R-3-(morfolinomethylen)-4H-1,2,4-triazole-5-yl)thio) acetic acid salts. Materials and methods. The objects of our research were 10 new substances derivative 1,2,4-triazole derivatives. Further it was studied the impact of these compounds on the performance and germination of the “Galatea” hybrid corn seed in 2016 harvest. We have used auxin as the study comparison and distilled water was used as a control. Results and discussion. The study had found that these compounds could differently influence on the growth and development of corn sprouts. It was mentioned that on the growth stimulating activity intensiveness can influence the replacement of amino group at the N4 nitrogen atom of 1,2,4-triazole cycle and the type of cation which is combined with acids. Conclusions. In the result of our experiment it was determined the prospects of further investigations of synthesized compounds as a growth stimulators and noted appropriateness of “structure-action” dependences could be integrated into future research


INTRODUCTION
Agricultural industry urgently requires new and effective growth stimulating remedies. These agents usually are natural or synthetic organic or inorganic compounds that in small concentrations can influence on the energy and speed of sprouts germination, length and weight of stems, germination which in turn affects the growth and development of plants helping to increase their productivity [1,2]. As growth stimulating substances for cereals and crops there are widely used numerous biologically active compounds from different classes. 1,2,4-triazole derivatives have had to exhibited themselves as active fungicides and growth stimulators (tryadimefon, cyproconazol, propiсonazol, penсonazol, dyfenoсonazol, tebuсonazol, epoksyсonazol etc.) [2][3][4]. Quite promising in this regard are the salts of 2-(4-R-3-R 1 -1,2,4-triazole-5-yltio) acetic acid [5]. Thus our previous research experience of growth stimulating activity of these substituents on the Sunflower Simple example indicates to the prospects of this research direction [5][6][7]. Important is the fact that along with labeled features the salts of 2-(4-R-3-R 1 -1,2,4triazole-5-ylthio)acetic acid do not show high acute toxicity and are not accumulating in humans and animals organisms [2][3][4]8].

MATERIALS AND METHODS
The objects of our research were 10 new substances Further it was studied the impact of these compounds on the performance and germination of the "Galatea" hybrid corn seed in 2016 harvest. For this study it was randomly counted 4 samples by 50 seeds and kept in a 0.01 % aqueous solution of these compounds for 6 hours. Then the seeds were washed three times with distilled water and then uniformly placed on moist filter paper in Petri dishes which were placed in a thermostatted cabinet in compliance with the temperature conditions of 20 ± 2 °C throughout the study period.
From the second day of the experiment we have counted the sprouted seeds referring to the fine grains those sprouts that have developed embryonic root not less than the length of a grain and formed germ which has at least half length of the seed. Sprouted seeds were placed in the box with filter paper are placed in cups of distilled water and kept in a thermostatically controlled cabinet.
The germination energy was calculated as a percentage of sprouted seeds on the third day of the experiment, seed germination was calculated on the seventh day.
Germination speed was calculated as the conventional index which indicates the number of days required for germination of 1 seed [1,9]. As the study comparison we have used β-indolyl acetic acid (Auxin) and distilled water was used as a control.
Thus in the result of the study we have marked compounds PKR-135, PKR-136 and PKR-177 which are virtually by all indicators of the growth stimulating activity excess comparison standard auxin. In the study of growth   (Table).

1.
We have conducted the studying of influence of 2-((4-R-3-(morfolinomethylen)-4H-1,2,4-triazole-5yl)thio) acetic acid salts on the growth and development of corn sprouts (Galatea hybrid) in laboratory conditions. 2. It was established that the most significant growth stimulating activity in corn sprouts showed inorganic salts (sodium PKR-136, potassium PKR-135 and ammonium PKR-177) of 2-((4-R-3-(morfolinomethylen)-4H-1,2,4-triazole-5-yl)thio) acetic acid. 3. In the result of our experiment it was determined the prospects of further investigations of synthesized compounds as a growth stimulators and noted appropriateness of "structure-action" dependences could be integrated into future research. Conflicts of Interest: authors have no conflict of interest to declare.