The study of the cytotoxic activity of the dry extract and the anthocyanin complex of Gebu Dahlia variety flowers




cytotoxic effect; spectrophotometry; dahlia; flowers; dry extract; anthocyanins


Topicality. For today, the search for promising plants with a high content of anthocyanins to create safe herbal medicines is an urgent problem of modern science.

Aim. To study the presence and severity of the cytotoxic activity of the dry extract of Gebu Dahlia variety flowers, the qualitative analysis and quantify the amount of anthocyanins in flowers of this variety.

Materials and methods. For the study, the dry extract of Gebu flowers was obtained. The cytotoxicity analysis of 1 %; 0.5 %; 0.25 %; 0.125 %; 0.0625 % solutions of the extract were performed by microscopy on red bone marrow (RBM) cells of rats at an exposure of 15, 45, 90 minutes. The spectrophotometric study and high-performance liquid chromatography were used to assess the quantitative and qualitative content of the amount of anthocyanins.

Results and discussion. The results obtained indicate that the viability of RBM cells is affected by the concentration of the extract and exposure: in the concentrations of 0.063-0.125 % no toxic effect of the extract was detected, while 0.25-1 % solutions were able to show cytostatic properties. The content of anthocyanin amount in the flowers was determined (1.8 % ± 0.02); 18 substances were identified, among them substances from the groups of cyanidin (54.7 %) and delphinidin (28 %) predominated.

Conclusions. The first stage of testing of the dry extract from Gebu Dahlia variety flowers for potential toxicity has been carried out: the extract is potentially non-toxic in the lowest of the concentrations studied. For the first time, the content of anthocyanins in flowers of Gebu Dahlia variety has been determined, 18 substances from the groups of delphinidin, cyanidin, petunidine, peonidine, malvidin have been identified.

Author Biographies

L. Maloshtan, National University of Pharmacy

Doctor of Biology (Dr. habil.), professor of the Biological Chemistry Department

L. Shakina, National University of Pharmacy

Candidate of Biology (Ph.D.), teaching assistant of the Physiology and Pathological Physiology Department

T. Gontova, National University of Pharmacy

Doctor of Pharmacy (Dr. habil.), professor, head of the Botany Department

S. Romanova, National University of Pharmacy

Candidate of Pharmacy (Ph.D.), teaching assistant of the Botany Department

M. Yaremenko , National University of Pharmacy

Рostgraduate student of the Botany Department


Barchuk, O. Z., Vronska, L. V. (2012). Farmatsevtychnyi chasopys, 1, 60–63. doi:

Motohashi, N. (Ed.). (2013). Anthocyanins: Structure, Biosynthesis and Health Benefits. Nova Science Publishers, 325.

Shesterin, V. I., Sevodin, V. P. (2013). Khimiia rastitelnogo syria, 3, 177–180. doi:

Rugină, D., Sconţa Z., Leopold L., Pintea A., Bunea A., Socaciu, C. (2012). Antioxidant activities of chokeberry extracts and the cytotoxic action of their anthocyanin fraction on HeLa human cervical tumor cells. Journal of medicinal food, 15 (8), 700–706. doi:

Reddy, N. M., Panda, K. K., Subhadra, A. V., Panda, B. B. (1995). The Allium micronucleus (MNC) assay may be used to distinguish clastogens from aneugens. Biol. Zent. bl., 114, 358–368.

Vinken, M., Blaauboer, B. J. (2017). In vitro testing of basal cytotoxicity: establishment of an adverse outcome pathway from chemical insult to cell death. Toxicol In Vitro, 39, 104–110. doi: 10.1016 / j.tiv.2016.12.004.

DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. (2014). Derzhavna Farmakopeia Ukrainy. (Vols. 1-3; Vol. 3). (2nd ed.). Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”, 732.

Meirelles, L. da S., Nardi, N. B. (2003). Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br. J. Haematol., 123 (4), 702–711. doi:

Zupanets, I. A., Vetrova, E. V., Sakharova, T. S., Dobrova, V. E. (2014). Vestnik VSU, Serie: Chemistry, Biology, Pharmacy, 3, 128–132. Available at:

Dobrova, V. Ye., Zupanets, I. A., Maloshtan, L. M., Stepanova, K. O. (2011). Klinichna farmatsiia, 15 (2), 18-21.

Gontova, T., Ilyinska, N., Golembiovska, O., Mashtaler, V. (2016). Study of the component composition of phenolic compounds obtained from Dahlia varieties Ken’s Flame herb. Der Pharma Chemica, 8 (18), 455–459.

Karlsen, A., Retterstol, L., Laake, P., Paur, I., Kjolsrud-Bohn, S., Sandvik, L., Blomoff, R. (2007). Anthocyanins Inhibit Nuclear Factor-kB Activation in Monocytes and Reduce Plasma Concentrations of Pro-Inflammatory Mediators in Healthy Adults. The Journal of Nutrition, 137, 1951–1954. doi:

Cooke, D., Schwarz, M., Boocock, D., Winterhalter, P., Steward, W. P., Gescher, A. J., Marczylo, T. H. (2006). Effect of cyanidin-3-glucoside and an anthocyanin mixture from bilberry on adenoma development in the ApcMin mouse model of intestinal carcinogenesis - Relationship with tissue anthocyanin levels. International journal of cancer, 119 (9), 2213–2220. doi:

Bellocco, E., Barreca, D., Giuseppin, L., Calderaro, A., El Lekhlifi, Z., Chebaibi, S., Smeriglio, A., Trombetta, D. (2016). Cyanidin-3-O-galactoside in ripe pistachio (Pistachia vera L. variety Bronte) hulls: Identification and evaluation of its antioxidant and cytoprotective activities. Journal of functional foods, 27, 376–385. doi:





Pharmacology and biochemistry