The study of the cytotoxic activity of the dry extract and the anthocyanin complex of Gebu Dahlia variety flowers
Keywords:cytotoxic effect; spectrophotometry; dahlia; flowers; dry extract; anthocyanins
Topicality. For today, the search for promising plants with a high content of anthocyanins to create safe herbal medicines is an urgent problem of modern science.
Aim. To study the presence and severity of the cytotoxic activity of the dry extract of Gebu Dahlia variety flowers, the qualitative analysis and quantify the amount of anthocyanins in flowers of this variety.
Materials and methods. For the study, the dry extract of Gebu flowers was obtained. The cytotoxicity analysis of 1 %; 0.5 %; 0.25 %; 0.125 %; 0.0625 % solutions of the extract were performed by microscopy on red bone marrow (RBM) cells of rats at an exposure of 15, 45, 90 minutes. The spectrophotometric study and high-performance liquid chromatography were used to assess the quantitative and qualitative content of the amount of anthocyanins.
Results and discussion. The results obtained indicate that the viability of RBM cells is affected by the concentration of the extract and exposure: in the concentrations of 0.063-0.125 % no toxic effect of the extract was detected, while 0.25-1 % solutions were able to show cytostatic properties. The content of anthocyanin amount in the flowers was determined (1.8 % ± 0.02); 18 substances were identified, among them substances from the groups of cyanidin (54.7 %) and delphinidin (28 %) predominated.
Conclusions. The first stage of testing of the dry extract from Gebu Dahlia variety flowers for potential toxicity has been carried out: the extract is potentially non-toxic in the lowest of the concentrations studied. For the first time, the content of anthocyanins in flowers of Gebu Dahlia variety has been determined, 18 substances from the groups of delphinidin, cyanidin, petunidine, peonidine, malvidin have been identified.
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