Prospects of creating a new medicine based on biologically active substances of bearberry leaves for correction of the metabolic syndrome

Authors

DOI:

https://doi.org/10.24959/ubphj.21.305

Keywords:

bearberry; leaves; dry extract; modification; hypoglycemic, hypolipidemic activity; metabolic syndrome

Abstract

Topicality. The metabolic syndrome is pathogenetically interrelated metabolic disorders in the condition of a sick person. A large set of factors is involved in its occurrence. Risk factors include genetic predisposition, overeating, insulin resistance, obesity, bad habits, hypodynamics, stress and unfavorable environmental conditions. First of all, defects of the lipid and carbohydrate metabolism provoke a cascade of genetic, metabolic, hormonal, nervous, inflammatory and other reactions and disorders in cells, tissues and organs, causing the metabolic syndrome and associated diseases, such as diabetes, kidney and gallstone disease, hypertension, platelet hyperaggregation, etc. Therefore, the rational use of synthetic and herbal medicines in the complex correction of these disorders can slow down the development of the metabolic syndrome.

Aim. To develop the method for obtaining a dry modified extract from bearberry, study its chemical composition, hypoglycemic and hypolipidemic activity in order to determine the prospects of its use for the correction of the metabolic syndrome.

Materials and methods. The study object was a dry extract of bearberry leaves modified with cysteine. HPLC and spectrophotometry were used to analyze the extract obtained. The hypoglycemic and hypolipidemic activity of dry extracts of bearberry was studied in rats with insulin resistance.

Results and discussion. The method for obtaining a dry modified extract from bearberry leaves was developed by adding cysteine. Phenologlycoside (arbutin), 2 phenolic acids (gallic and ellagic), 6 flavonoids, 8 saponins were identified in the extract, and their quantitative content was determined. Hyperoside and catechin were dominant among flavonoids, and ursolic acid, uvaol, and lupeol prevailed among saponins. The content of the main groups of phenolic compounds was determined in the extract by spectrophotometry. The introduction of the dry extract from bearberry leaves modified with cysteine has a normalizing effect on metabolic disorders on the background of a high-fructose diet; therefore, it can be a promising agent for the correction of the metabolic syndrome.

Conclusions. As a result of the research conducted, a new dry extract from bearberry leaves modified with cysteine has been created. The phytochemical composition, hypoglycemic and hypolipidemic activities of the extract have been studied, indicating the prospects for its use to correct the metabolic syndrome.

Author Biographies

N. Chaika, National University of Pharmacy

Postgraduate student of the Pharmacognosy Department

O. Koshovyi, National University of Pharmacy

Doctor of Pharmacy (Dr. habil.), professor, head of the Pharmacognosy Department

M. Mazen, National University of Pharmacy

Postgraduate student of the Biological Chemistry Department

A. Kravchenko, National University of Pharmacy

Candidate of Biology (Ph.D.), associate professor of the Biological Chemistry Department

A. Kovalyova, National University of Pharmacy

Doctor of Pharmacy (Dr. habil.), professor of the Pharmacognosy Department

I. Kireyev, National University of Pharmacy

Doctor of Medicine (Dr. habil.), professor, Director of the Educational and Scientific Institute of Applied Pharmacy

References

Metabolic syndrome. National Heart, Lung, and Blood Institute. Available at: https://www.nhlbi.nih.gov/health-topics/metabolic-syndrome.

Hu, X., Li, X., Adams, H., Kubena, K., Guo, S. (2019). Etiology of Metabolic Syndrome and Dietary Intervention. International Journal of Molecular

Sciences, 20 (1), 19. doi: https://doi.org/10.3390/ijms20010128.

Samson, S. L., Garber, A. J. (2014). Metabolic syndrome. Endocrinology and Metabolism Clinics of North America, 43 (1), 1–23. doi: https://doi.

org/10.1016/j.ecl.2013.09.009.

Ferri, F. F. (2019). Metabolic syndrome. Ferri’s Clinical Advisor. 2019. Philadelphia, Pa.: Elsevier.

Rochlani, Y., Pothineni, N. V., Kovelamudi, S. Mehta, J. L. (2017). Metabolic syndrome: pathophysiology, management, and modulation by natural

compounds Therapeutic Advances in Cardiovascular Disease, 11 (8), 215–225. doi: https://doi.org/10.1177/1753944717711379.

Saklaeyn, M.G. (2018). The Global Epidemic of the Metabolic Syndrome. Current Hypertension reports, 20 (12). doi: https://doi.org/10.1007/

s11906-018-0812-z.

Koshovyi, O. M., Zagayko, A. L., Kolychev, I. O., Akhmedov, E. Yu., Komissarenko, A. N. (2016). Phytochemical study of the dry extract from bilberry

leaves. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 16 (1), 18–23.

Zagayko, A. L., Kolisnyk, T. Y., Chumak, O. I., Ruban, O. A., Koshovyi, O. M. (2018). Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model. Journal of Basic and Clinical Physiology and Pharmacology, 29 (6), 697–703.

doi: https://doi.org/10.1515/jbcpp-2017-0161.

Koshovyi, O., Komissarenko, M., Zagayko, A., Ilina, T. (2016). Prospects of creating a new medicine with the hypoglycemic action from cowberry

leaves. London Review of Education and Science, “Imperial College Press”, 1 (19), 1016–1021.

Koshovyi O. M., Stremoukhov, O. O., Kravchenko, H. B., Krasilnikova, O. A., Zagayko, A. L., Komissarenko, M. A. (2020). Sposib oderzhannia likuvalno-profilaktychnoho zasobu z lystia lokhyny dlia korektsii ta profilaktyky metabolichnoho syndrome: Patent Ukrainy 145107. № u 2020 02993.

Byul., 22.

Parejo, I., Viladomat, F., Bastida, J., Codina C. A. (2001). A Single Extraction Step in the Quantitative Analysis of Arbutin in Bearberry (Arctostaphylos uva-ursi) Leaves by High-Performance Liquid Chromatography. Phytochem. Anal., 12 (5), 336–339. doi: https://doi.org/10.1002/pca.602.

Radulović, N., Blagojević, P., Palić, R. (2010). Comparative Study of the Leaf Volatiles of Arctostaphylos uva-ursi (L.) Spreng. And Vaccinium vitis-idaea L.

(Ericaceae).Molecules, 15 (9), 6168–6185. doi: https://doi.org/10.3390/molecules15096168.

Linderborg K., Laaksonen O., Kallio H., Yang, B. (2011). Flavonoids, sugars and fruit acids of alpine bearberry (Arctostaphylos alpina) from Finnish

Lapland. Food Research International., 44 (7), 2027–2033. doi: https://doi.org/10.1016/j.foodres.2010.10.036.

Amarowicz, R., Pegg, R.B., Kosińska, A. (2008). Chromatographic separation of tannin fractions from a bearberry-leaf (Arctostaphylos uva-ursi L.

Sprengel) extract by HPLC – a short report. Pol. J. Food Nutr. Sci., 58 (4), 485–490.

Koshovyi, O., Raal, A., Kireiev, I., Tryshchuk, N., Ilina, T., Romanenko, Y., Kovalenko, S. M., Buniatian, N. (2021). Phytochemical and Psychotropic

Research of Motherwort (Leonurus cardiaca L.) Modified Dry Extracts. Plants, 10 (2), 230. doi: https://doi.org/10.3390/plants10020230.

Shinkovenko, I. L., Kashpur, N. V., Ilina, T. V., Kovalyova, A. M., Goryacha, O. V., Koshovyi, O. M. et al. (2018). The immunomodulatory activity of the extracts

and complexes of biologically active compounds of Galium verum L. Herb. Ceska a Slovenska Farmacie, 67 (1), 25–29.

Koshovyi О. М. (2010). Farmacom, 3, 27–31.

Ilina, T., Skowronska, W., Kashpur, N., Granica, S., Bazylko, A., Kovalyova, A., Goryacha O., Koshovyi, O. (2020). Immunomodulatory Activity and

Phytochemical Profile of Infusions from Cleavers Herb. Molecules, 25 (16), 3721. doi: https://doi.org/10.3390/molecules25163721.

Shynkovenko, I. L., Ilina, Т. V., Kovalyova, А. М., Goryacha, O. V., Golembiovska, O. I., Koshovyi О. M. (2018). Saponins of the extracts of Galium

aparine and Galium verum. News of Pharmacy, 4 (96), 16–23. doi: https://doi.org/10.24959/nphj.18.2225.

Myha, M. M., Koshovyi, O. M., Hamulia, O. V., Verkhovodova, Yu. V., Kireiev, I. V., Komisarenko, A. M. (2020). Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 1 (32), 61–71. doi: https://doi.org/10.14739/2409-2932.2020.1.198136.

DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. (2015). Derzhavna Farmakopeia Ukrainy. (Vols. 1-3; Vol. 1). (2nd ed.).

Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”, 1128.

Vovk, H. V., Myha, M. M., Koshovyi, O. M., Verkhovodova, Yu. V., Kireiev I. V. (2016). Ukrainskyi biofarmatsevtychnyi zhurnal, 1 (42), 51–54.

doi: https://doi.org/10.24959/ubphj.16.10.

Stefanov, A. V. (Ed.). (2002). Doklinicheskie issledovaniya lekarstvennykh sredstv. Kiev: Avitsena, 568.

Charradi, K., Elkahoui, S., Karkouch, I., Limam, F., Hassine, F. B., El May, V., Aouani, E. (2014). Protective effect of grape seed and skin extract against

high-fat diet-induced liver steatosis and zinc depletion in rat. Digestive Diseases and Sciences, 59 (8), 1768–1778. doi: https://doi.org/10.1007/

s10620-014-3128-0.

Published

2021-04-12

Issue

Section

Pharmacology and biochemistry