State of biochemical system of white rat’s blood in conditions of alcoholic hepatic injury and preventive action metadoxine

Authors

  • G. M. Shayakhmetova SI “Institute of Pharmacology & Toxicology of the NAMS of Ukraine, Ukraine
  • O. V. Karpova A. V. Bogatsky Physico-chemical Institute of the NAS of Ukraine, Ukraine
  • A. K. Voronina SI “Institute of Pharmacology & Toxicology of the NAMS of Ukraine, Ukraine
  • N. Y. Golovenko A. V. Bogatsky Physico-chemical Institute of the NAS of Ukraine, Ukraine

DOI:

https://doi.org/10.24959/ubphj.16.18

Keywords:

metadoxine, alcoholic hepatitis, hepatic protector

Abstract

It was developed model of alcoholic hepatitis by intragastric administration of 40 % ethanol at a dose of 7 ml/kg body weight of male Wistar rats for 7 days. As a biochemical marker of alcoholic hepatitis were used indicators of cytolytic syndrome (activity of alanine aminotransferase, aspartate aminotransferase, catalase), cholestatic syndromes (alkaline phosphatase, gamma glutamyl transpeptidase) and lipid exchange (levels of cholesterol, triglycerides). It was shown that metadoxine (ionic salt of pyridoxine and pyroglutamic acid), which was administered preventively intragastrically at a dose of 90 mg/kg 30 min prior to the administration of alcohol, normalized activity of aspartate aminotransferase and catalase; gamma-glutamyl transpeptidase; triglycerides. The lack of a positive influence of metadoxine on the other studied biochemical blood parameters indicatives that mechanisms of development of alcoholic hepatitis and course of its are different. It is assumed that the hepatoprotective effect of the drug is realized through antioxidant mechanism.

References

Бабак О. Я. Хронические гепатиты / Бабак О. Я. – К. : Блиц-Информ, 1999. – 208 с.

Болезни печени и желчевыводящих путей : руководство для врачей / под ред. В. Т. Ивашкина. – [2-е изд.]. – М. : Медицина, 2005. – 536 c.

Буров Ю. В. Биологические модели хронического алкоголизма / Ю. В. Буров, В. Н. Жуков // Итоги науки и техники (серия Токсикология). – 1984. – Т.13. – С. 57–92.

Доклінічні дослідження лікарських засобів : методичні рекомендації / за ред. О. В. Стефанова. – К. : Авіцена, 2001. – 528 с.

Разработка экспериментальных моделей алкогольного гепатита различной степени тяжести / А. Н. Петров, М. К. Шевчук, Е. К. Георгианова [и др.] // Medline.ru. – 2015. – № 16. – С. 187–202.

Способ определения активности каталазы / М. А. Королюк, Л. И. Иванова, И. Г. Майорова [и др.] // Лабораторное дело. – 1991. – № 10. – С. 9–13.

Сухарева Г. В. Алкогольная болезнь печени / Г. В. Сухарева // Consilium medicum. Гастроэнтерология. – 2003. – Т. 5, № 3. – С. 26–27.

Шульпекова Ю. О. Лекарственные поражения печени / Ю. О. Шульпекова // Врач. – 2010. - № 7. – С. 10–13.

Acute alcohol intoxication / L. Vonghia, L. Leggio, A. Ferrulli [et al.] // European Journal of Internal Medicine. – 2008. – 19, № 8. – Р. 561–567.

Efficacy of metadoxine in the management of acute alcohol intoxication / Díaz Martínez MC, Díaz Martínez A., Villamil Salcedo V. [et al.] // Journal of International Medical Research. – 2002. – 30, № 1. – Р. 44–51.

Gebo K. A. Role of liver biopsy in management of chronic hepatitis C: a systematic review / K. A. Gebo, H. F. Herlong, M. S. Torbenson // Hepatology. – 2002. – 36 (Suppl. 1). – Р.161–172.

Kondo K. Enhancement of APAP-induced chronic hepatotoxicity in restricted fed rats: nonclinical approach to APAP-induced chronic hepatotoxicity in susceptible patients / K. Kondo // Journal Of Toxicological Sciences. – 2012. – 37, №5. – P. 911–929.

Metadoxine in acute alcohol intoxication: a double-blind, randomized, placebo-controlled study / L. S. Shpilenya, A. P. Muzychenko, G. Gasbarrini [et al.] // Alcoholism: Clinical and Experimental Research. – 2002. – 26, № 3. – Р. 340–346.

Metadoxine in the treatment of acute and chronic alcoholism: a review / G. Addolorato , C. Ancona , E. Capristo [et al.] // International Journal of Immunopathology and Pharmacology. – 2003. – 16, № 3. – Р. 207–214.

Nawasurit P. Ethanol–induced free radicals and hepatic DNA strand breaks are prevented in vivo by antioxidants / P. Nawasurit, T.H. Ward, N.J.F. Dodd // Carcinigenesis. – 2000. – 21. – P. 93–99.

Paumgartner G. Medical treatment of cholestatic liver diseases: From pathobiology to pharmacological targets / G. Paumgartner // World Journal of Gastroenterology. – 2006. – 12, № 28. – Р. 4445–4451.

Tokeyama Y. Role of Kupfer cell-derived reactive oxygen intermediates in alcoholic liver disease in rats in vivo / Y. Tokeyama, S. Kamimura, A. Kuroiwa [et al.] // Alcoholism: Clinical and Experimental Research. – 1996. – V.20 (Suppl. 9). – P. 335–339.

Downloads

Published

2016-04-12

Issue

No. 2(43) (2016)

Section

Pharmacology and biochemistry

License

Copyright (c) 2016 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).