Development of the method for quantitative determination of diclophenac sodium in rat plasma by high-performance liquid chromatography with regard to analysis characteristics of active substance micro-concentrations

Authors

  • L. V. Brun National University of Pharmacy, Ukraine
  • S. N. Gubar National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/ubphj.16.40

Keywords:

nonsteroidal anti-inflammatory drugs, diclofenac sodium, glucosamine, high-performance liquid chromatography

Abstract

For the study of quantitative determination of diclofenac sodium, sample preparation of biological objects has been performed. Linear dependence of diclofenac sodium peak area and substance concentration in the solution (5-25 mg/ml) has been proved. The method of solid phase extraction (SPE) has been selected as the extraction method. The analysis has showen that concentration of diclofenac sodium in the samples was in the range of 2-4 mg/ml. A method for quantitative determination of diclofenac sodium in rat plasma samples by high-performance liquid chromatography (HPLC) with regard to analysis characteristics of active substance micro-concentrations has been developed. 

References

Доклінічні дослідження лікарських засобів / За ред. О.В.Сефанова: Метод. рекомендації.  К.: Авіцена, 2001.  С. 296-297.

Вивчити вплив комплексного застосування низькоінтенсивногоінфрачервоного лазерного випромінювання та медичних препаратів на перебіг остеоартрозу за фрагментом „Вивчити фармакокінетику диклофенаку натрію та глюкозаміна гідрохлорида під впливом інфрачервоної лазеротерапії в експерименті на тваринах в залежності від послідовності їх застосування”: звіт про НДР (заключний): кер. І.А. Зупанець; виконавець Л.В. Брунь та ін.. Харків, 2010. – 68 с. – Инв. № 756600.

Компендиум 2015 – лекарственные препараты / Под ред. В.Н.Ко¬ва-ленко, А.П.Викторова. – К.: Морион, 2015. – 2270 с.

Лапач С.Н., Чубенко А.В., Бабич П.Н. Статистические методы в медико-биологических исследованиях с использованием Excel. – К.: Морион, 2000. – 320 с.

Лобенко О.О., Корж М.О., Дєдух Н.В., Зупанець Ф.А. та спів. Остеоартроз. Консервативна терапія / За ред. М.О.Коржа, Дєдух Н.В., Зупанця І.А. – Харьков: Прапор, 1999. – 336 с.

Лоуренс Д.Р., Бенитт П.Н.. Клиническая фармакология. М., Меди-цина, 1991; т.1: 485–524.

Brunner L.A., Luders R.S. An automated method for the determination of diclofenac sodium in human plasma // J. Chromatogr. Sci. – 1991. – Vol.29, № 7. – Р. 287-291.

Flow-injection spectrophotometric determination of diclofenac sodium in pharmaceuticals and urine samples / M.S.Garcia, M.I.Albero, C.Sanchez-Pedreno, J.Molina // J.Pharm.Biomed.Anal. – 1998. – Vol.17, № 2. – Р. 267-273.

In vivo bioequivalence guidances (1090) – U.S. Pharmacopeia 23, NF 18. – 1995. – P. 3028 – 3053.

Kamath B.V., Shivram K., Shah A.C. High-perfomance liquid chromatographic method for the determination of diclofenac in human plasma and urine // J. Chromatogr. – 1993. – Vol.12, № 3. – Р. 324-329.

Simultaneous determination of diclofenac and oxybuprocaine in human aqueous humor with HPLC and electrochemical detection / O. Kuhlmann, G. Stoldt, H. G. Struck, G. J. Krauss // J. Pharm. Biomed. Anal. – 1998. – Vol.17, № 8. – Р. 1351-1356.

United States Pharmacopea / Official Monographs. – 1995. – P. 546. 13. Zecca L., Ferrario P., Costi P. Determination of diclofenac and its metabolites in plasma and cerebrospinal fluid by high- perfomance liquid chromatography with electrochemical detection // J. Chromatogr. – 1991. – Vol.8, № 2. – Р. 425-432.

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Published

2016-06-13

Issue

No. 3(44) (2016)

Section

Pharmacology and biochemistry

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