Development of the methodology of the chromatographic determination of verapamile hydrochloride in medicines
Keywords:verapamil hydrochloride, high-Performance liquid chromatography, validation, linearity, accuracy, range of application
AbstractObjective: The objective of this research was to develop more simple, sensitive, accurate and less expensive analytical methods for the determination of verapamil hyrdochloride in medicines by HPLC. Methods: The chromatographic analysis of verapamil hyrdochloride performed on liquid chromatograph Agilent 1290 Infinity II LC System. Results: In developing our technique, column Nucleosil C18 was used, which has several advantages from a number of columns L1 and provides high speed and high efficiency at a lower pressure system. This reduces the number of used mobile phase that according reduces the cost analysis. Selected conditions were isocratic elution with binary mobile phase consisting of methanol, water, acetic acid and triethylamine in the ratio 55 : 44 : 1 : 0.1 for optimum peak symmetry of the active pharmaceutical ingredient. Under this conditions the peak of verapamil elution is about 2 minutes. Besides reducing analysis time achieved by simplifying sample preparation by using methanol as solvent and mobile phase. The proposed method has the advantage over pharmacopoeial method due to speed and ease of preparation of the mobile phase and reduced chromatography time. Conclusion: The results obtained in this research work clearly indicated that the proposed method is rapid, economical, simple, accurate, selective, precise and applicable to the analysis of verapamil hyrdochloride in medicines.
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