Glutathione content and enzymes’ activity of its metabolism in rats’ liver during longlasting Action of oligoesters of polyatomic alcohol

A. V. Bondareva, S. O. Stetsenko

Abstract


Study of mechanisms of pathological process development of xenobiotics action is actual in modern science. Oligoesters of polyatomic alcohol “Laprols” present widespread xenobiotics, which are characterized by significant synthesis volume, wide use (as basis of industrial output of synthetic resin, foam polyurethane, paint materials, household agents), intake in water sources of population and as a result impact on person’s health. Defined ways of organism adaptation to xenobiotics action were formed during evolution, central role in which presents biochemical mechanisms of their decontamination. The aim of the investigation is to evaluate the influence of oligoesters of polyatomic alcohol “Laprols” OEF-LP-502 in doses 1/10 and 1/100 LD50 on glutathione content and enzymes’ activity of its metabolism in rats’ liver. Experiments were done on sexually mature rats of Wistar with body weight (180-220) g. They were exposed to per oral coarse with probe by water solution of compounds daily and once time during 45 days in doses 1/10 and 1/100 LD50. Animals of control group were taken volumes of fresh water. Investigation of indices was done in dynamics on the 15-th, 30-th, 45-th days after experiment’s start. After 45-day administration of OEF by rats in doses 1/10 and 1/100 LD50 abnormalities in the glutathione system develop in the liver, which are manifested by reduced content of restored glutathione, the activity of enzymes that destroy hydroperoxide (glutathione peroxidase and G-S-T) on the background of glutathione reductase increase as an adaptive response to renew restored glutathione. Changes of glutathione content and activity of enzymes of its metabolism in conditions of prolonged influence of OEF is the reason for the intensification of lipid peroxidation and degradation as a result of hepatocytes of rats. Abnormalities of glutathione system in hepatocytes of rats are one of the pathogenetic links of the mechanisms of action of OEF-LP that should be considered in the development of methods for their correction.

Keywords


glutathione; xenobiotics; oligoesters polyols

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DOI: https://doi.org/10.24959/ubphj.16.65

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Abbreviated key title: Ukr. bìofarm. ž.

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