Determination of liposome internal volume with irinotekan


  • A. V. Stadnichenko National Technical University “Kharkov Polytechnic Institute”, Ukraine
  • Yu. M. Krasnopolskyi National Technical University “Kharkov Polytechnic Institute”, Ukraine
  • V. I. Shvets Moscow State University of fine chemical technology named by M. V. Lomonosov, Russian Federation
  • T. G. Yarnykh National University of Pharmacy, Ukraine



liposomes, irinotecan, determination of internal volume, HPLC


Were obtained liposomes with irinotecan by “pH gradient” method. To determine the internal volume of liposomes used control method of citrate ion in ultrafiltrate by the HPLC. It was determined that the internal volume of liposomes obtainedis 10.15 % of the total emulsion volume. The method can be used in the development of liposomes by “pH gradient” using citric acid as an internal buffer.


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Biopharmaceutical research