Beer with the pine needles extract addition toxic effect research

L. Tatar, N. Penkina


Topicality. Alcohol is one of the risk factors for many diseases, including coronary heart disease, stroke, type 2 diabetes mellitus and others. Common world ways in solving this problem in recent years has improved the preparatory technology quality of alcohol and their composition optimization, leading to lessening the negative alcohol effects on the body.
Aim. To investigate the beer toxicological aspects and assess the feasibility of creating a new brewing technology with the addition of an aqueous pine needles extract, which partly replaced by hops.
Material and methods. Beer prepared on the classical recipe with the addition of pine extract (600 ml/dl). The influence of beer with the pine needle extract addition on prooxidant-antioxidant status of the animals liver comparing with the beer produced by the classical recipe.
Results and discussion. The animals were given the beer examples produced by classical technology at a dose of 15 g/kg for a period of 14 days significantly increased the content of oxidative markers in the liver homogenate (GC 20.4 %, TBA-reagents – 51.6 %) compared to animals that received placebo and reduced activity catalase – by 40.9 %. Biomarkers in liver homogenate of animals treated beer with an pine needles extract were within the physiological norm. Under the influence of prednisolone (50 mg/kg) dien conjugates content in the liver of animals treated with classical technologies beer by 23.8 % more than in animals that under conditions of oxidative stress used purified water. The content of TBA-reagents over 13.7 %, glutathione content less than 25.0 % compared to the placebo group. In animals treated with beer extract of pine needles all anti-oxidative markers of liver catalase activity are significantly differed from that of animals that used purified water. In animals treated with the classic beer technology under normobaric hypoxia model was likely reduce the average lifetime to 313.1 s in terms of lack of oxygen compared to animals treated with purified water. In animals administrated with beer extract of pine needles average lifetime remained intact at figures and had no probable deviations.
Conclusions. According to biochemical markers research of anti-oxidative system in liver homogenate of animals, beer with pine needles extract, due to its antioxidative properties, minimizes the negative alcohol impact on the liver
that manifested in less oxidative stress. Beer with pine needles extract does not potentiate the adverse effects of alcohol in acute hypoxic condition and does not reduce the average lifetime of the animal, as opposed to the usual beer. A beer
“Emerald” with an extract of pine needles, due to its antioxidant properties, may be an alternative to modern alcoholic beverages and significantly reduce the toxic effects of alcohol.


pine needles; beer; antioxidant properties; oxidative stress

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