Aromatase inhibitors effect on the total protein content of visceral adipose tissue in hamsters with metabolic syndrome
DOI:
https://doi.org/10.24959/ubphj.17.127Keywords:
aromatase, inhibitors, metabolic syndrome, letrozole, anastrozole, exemestaneAbstract
Topicality. Recently, more attention has been paid to the role of the peripheral aromatase reaction enhancement in pathogenesis of obesity and metabolic syndrome. About 39 % of men and 40 % of women over 18 around the world are overweight (BMI > 25) and in almost all cases they have an imbalance of sex hormones, which is caused by increased aromatase activity of adipose tissue.
Aim. To study the effect of aromatase inhibitors on the parameters of total body weight, protein content in adipose tissue and their correlation in hamsters with experimental metabolic syndrome.
Materials and methods. Protein content in the visceral adipose tissue homogenate was detected by the Lowry method in the Hartree modification. Statistical processing of the results was carried out by variational statistics methods using nonparametric methods of analysis, the interrelations between the signs were determined by the Pearson linear correlation coefficient.
Results and discussion. The most effective drug from the group of aromatase inhibitors which reduced hypertrophic growth of adipose tissue in all animals both sexes and ages was letrozole. Anastrozole and exemestane showed less efficacy, and had a better effect on young animals.
Conclusions. The letrozole administration at a dose of 0.309 mg/kg resulted in a significant decrease in the average body weight by 24-35 % and a corresponding increase in protein content of visceral adipose tissue by 12-46 % with a high correlation coefficient.
References
Bulun, S. E., Chen, D., Moy, I., Brooks, D. C., Zhao, H. (2012). Aromatase, breast cancer and obesity: a complex interaction. Trends in Endocrinology & Metabolism, 23 (2), 83–89. doi: 10.1016/j.tem.2011.10.003
Boonchaya–anant, P., Laichuthai, N., Suwannasrisuk, P., Houngngam, N., Udomsawaengsup, S., Snabboon, T. (2016). Changes in Testosterone Levels and Sex Hormone–Binding Globulin Levels in Extremely Obese Men after Bariatric Surgery. International Journal of Endocrinology, 2016, 1–5. doi: 10.1155/2016/1416503
Cao, J., Chen, T., Hao, W., Li, J., Liu, L., Zhu, B., Li, X. (2012). Correlation between sex hormone levels and obesity in the elderly male. The Aging Male, 15 (2), 85–89. doi: 10.3109/13685538.2012.666585
Overweight and obesity (2014). Global Health Observatory (GHO) data. Available at: http://www.who.int/gho/ncd/risk_factors/overweight_text/en/
Sutton–Tyrrell K., Zhao X., Santoro N. et al. (2010). Reproductive hormones and obesity: 9 years of observation from the Study of Women’s Health Across the Nation. Am. J. Epidemiol., 171 (11), 1203–1213. doi: 10.1093/aje/kwq049
Gunter, M. J., Hoover, D. R., Yu, H. et al. (2009). Insulin, insulin–like growth factor–I, and risk of breast cancer in postmenopausal women. J. Natl. Cancer Inst., 101, 48–60.
Morimoto, L. M., White, E., Chen, Z. et al. (2002). Obesity, body size, and risk of postmenopausal breast cancer: the Women’s Health Initiative (United States). Cancer Causes Control, 13, 741–751.
De Waars, F., Baanders–Van Halewijn, E. A., Huizinga, J. (1964). The bimodal age distribution of patients with mammary carcinoma: evidence for the existence of 2 types of human breast cancer. Cancer, 17 (2), 141–151. doi: 10.1002/1097–0142(196402)17:2<141::aid–cncr2820170202>3.0.co;2–z
Bulun, S. E. (2005). Regulation of Aromatase Expression in Estrogen–Responsive Breast and Uterine Disease: From Bench to Treatment. Pharmacological Reviews, 57 (3), 359–383. doi: 10.1124/pr.57.3.6
Lin, Z., Yin, P., Reierstad, S., O’Halloran, M., Coon V, J. S., Pearson, E. K., Bulun, S. E. (2009). Adenosine A1 receptor, a target and regulator of estrogen receptorα action, mediates the proliferative effects of estradiol in breast cancer. Oncogene, 29 (8), 1114–1122. doi: 10.1038/onc.2009.409
Chlebowski R.T., Anderson G.L., Gass M. et al. (2010). Estrogen plus progestin and breast cancer incidence and mortality in postmenopausal women. JAMA, 304, 1684–1692.
Pasquali, R. (2003). Obesity and reproductive disorders in women. Human Reproduction Update, 9 (4), 359–372. doi: 10.1093/humupd/dmg024
Pasquali, R. (2006). Obesity and androgens: facts and perspectives. Fertility and Sterility, 85 (5), 1319–1340. doi: 10.1016/j.fertnstert.2005.10.054
Thomas, L. W. (1962). The chemical composition of adipose tissue of man and mice. Quarterly Journal of Experimental Physiology and Cognate Medical Sciences, 47 (2), 179–188. doi: 10.1113/expphysiol.1962.sp001589
Gersh, I.(1945). Blood vessels in fat tissue. Relation to problems of gas exchange. Journal of Experimental Medicine, 81 (2), 219–232. doi: 10.1084/jem.81.2.219
Kozhemiakin, Yu. M., Khromov, O. S., Filonenko, M. A., Saifetdinova, H. A. (2002). Naukovo–praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv: Derzhavnyi farmakolohichnyi tsentr MOZ Ukrainy, 155.
17. Guide for the care and use of laboratory animals, 8th edition. (2011).Washington : The National Academies Press, 246.
18. European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe (1986).Strasbourg, 52.
Good Laboratory Practice. (2005). OECD principles and guidance for compliance monitoring. OECD.
Kasim–Karakas, S. E., Vriend, H., Almario, R., Chow, L.–C., Goodman, M. N. (1996). Effects of dietary carbohydrates on glucose and lipid metabolism in golden Syrian hamsters. Journal of Laboratory and Clinical Medicine, 128 (2), 208–213. doi: 10.1016/s0022–2143(96)90013–x
Wong, S. K., Chin, K.–Y., Suhaimi, F. H., Fairus, A., Ima–Nirwana, S. (2016). Animal models of metabolic syndrome: a review. Nutrition & Metabolism, 13 (1). doi: 10.1186/s12986–016–0123–9
Nair, A., Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7 (2), 27. doi: 10.4103/0976–0105.177703
Stefanov, A. V. (2002). Doklinicheskie issledovaniia lekarstvennykh sredstv.Kiev: Avicenna, 528.
Shabanova, N. A. (1984). Metodicheskie ukazaniia k laboratornym zaniatiiam spetckursa «Obmen aminokislot».Kharkov: Rotary Prints of KhGU, 74.
Lapach, S. N., Chubenko, A. V., Babich, P. N. (2000). Statisticheskie metody v medico–biologicheskikh issledovaniiakh s ispolzovaniiam Excel.Kiev: Morion, 320.
Rebrova, O. Yu. (2006). Statisticheskii analiz meditcinskikh dannykh. Primenenie paketa prikladnykh program STATISTICA, 3rd ed.Moscow: MediaSfera, 312.
Anderson, L. C., Otto, G., Pritchett–Corning, K. R., Whary Elsevier, M. T. (2015). Laboratory Animal Medicine, 1746.
Klöting, N., Blüher, M. (2014). Adipocyte dysfunction, inflammation and metabolic syndrome. Reviews in Endocrine and Metabolic Disorders, 15 (4), 277–287. doi: 10.1007/s11154–014–9301–0
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