Investigation of anti-microbial activity of diourethical herbal collection

Authors

  • O. H. Doroshenko I. Horbachevsky Ternopil State Medical University
  • S. M. Marchyshyn I. Horbachevsky Ternopil State Medical University
  • N. I. Tkachuk I. Horbachevsky Ternopil State Medical University

DOI:

https://doi.org/10.24959/ubphj.17.130

Keywords:

diuretic herbal collection, antimicrobial activity, disco-diffusion method, serial dilution method

Abstract

Topicality. The problems of inflammatory diseases of kidneys and urinary tract are some of the most difficult in the modern urology. The necessity for long-term use of drugs in the conservative treatment of non-specific inflammatory
diseases of the urogenital system allows prefer herbal preparations. It is known that urogenital infections (urethritis, cystitis, prostatitis, pyelonephritis, etc.) are more commonly caused by opportunistic bacteria whitch can penetrate into
the urogenital system by ascending through the urethra, hematogenous pathway, or after traumas of the pelvic organs, urinary system. Mostly such penetration of bacteria into the urinary system ends with their elimination from the body. However, with violations of general reactivity, immunodeficiencies, a large infectious dose of bacteria, they initiate the infectious process. In various uroinfections from the urine, mainly gram-negative microorganisms of different genera and species are sown: E. coli, Proteus spp., Enterobacter spp., Klebsiella spp., P. aeruginosa, non-clostridial anaerobes (Vacteroides spp., Prevotella spp.), and also fungi of the genus Candida. Should pay attention also to the importance of gram-positive coca flora, in the first place, S. epidermidis, S. aureus, S. pyogenes, S. fecalis, Enterococus spp.
Aim. To determine the antimicrobial activity of the diuretic herbal collection, which includes Polygoni avicularis herba, Fragariae herba, Juglans folia, Uvae-ursi folia, Urticae folia, Elytrigia rhizomata et radices (by 25.0), Helichrysi flores (20.0).                                                                                                          Materials and methods. Antimicrobial sensitivity studies were conducted using disco-diffusion method and method of serial dilutions. As a test cultures were selected the museum strains of E. coli, P. aeruginosa, Kl. pheumoniae, B. cereus, Corynebacterium spp., Candida spp., S. aureus, S. epidermidis.
Results and discussion. The results of experiments conducted from the study of antimicrobial activity of samples in relation to different cultures of microorganisms have shown that all of them exhibit bacteriostatic action only to grampositive flora. It has been established that diuretic herbal collection in dilution 1 : 5, diuretic herbal tea and nephrophyte in dilution 1 : 2 exhibited bacteriostatic effects in relation to B. cereus, Corynebacterium spp., Candida spp., S. epidermidis, whereas 3 types of gram-negative bacteria (E coli, P. aeruginosa, C. pneumoniae) were resistant to them.
Conclusions. Antimicrobial activity of diuretic herbal collection, diuretic herbal tea, nephrophyte was investigated and three of them showed bacteriostatic action on gram-positive microflora, which allows to recommend them for application in the complex therapy of infectious diseases of the urinary system.

Author Biographies

O. H. Doroshenko, I. Horbachevsky Ternopil State Medical University

Doctor of Pharmacy, Professor, Head of the Department of Pharmacognosy and Medical Botany

S. M. Marchyshyn, I. Horbachevsky Ternopil State Medical University

the candidate of the Department of Pharmacognosy with Medical Botanist

N. I. Tkachuk, I. Horbachevsky Ternopil State Medical University

PhD, Associate Professor of the Department of Microbiology, Virology and Immunology

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Published

2017-10-17

Issue

Section

Microbiological research