DOI: https://doi.org/10.24959/ubphj.17.133

Investigation of the term of amlodipine storage in biological material in its putrefactive decomposition

O. O. Mamina, V. I. Kabachny, T. O. Tomarovska

Abstract


Topicality. Amlodipine besylate belongs to the group of calcium channel blockers, derivative from 1,4-dihydropyridine. The pharmacological effects of amlodipine are the cause its effectiveness in the treatment of hypertension and the vasospastic form of stenocardia. According to the literature sources, amlodipine in case of overdose can provoke the development of breast cancer, cause ischemia of the optic nerve. Deadly poisoning with amlodipine may accompany drug overdoses or suicidal cases. The development of highly sensitive and selective methods for the study of amlodipine during forensic toxicological examination of biological material is an actual task.
Aim. To develop an algorithm for the direct analysis of amlodipine in putrefactive biological material suitable for forensic toxicological examination and the study of the term of storage of the test substance.
Materials and methods. For the study, model mixtures of liver tissue with amlodipine besylate were used, which were stored at 5 °C for 1, 10, 20, 30 and 40 days. At the same time, control experiments were put. Extraction of the substance was carried out by the modified Stas-Otto method, purification was performed by extraction of impurities with diethyl ether at a pH of 2,0-3,0 and TLC method. Amlodipine was identified in biogenic extracts by the TLC method in
three mobile solvent systems. The content of the substance was determined by spectrophotometry in the UV spectral region and extraction photometry.
Results and discussion. It was found that after storage in the liver tissue, amlodipine can be isolated by the modified Stas-Otto method – 20.0-23.3 % (ε = ± 3.01-4.87 %, RSDx = 22.32-38.84 %). After 20 days of storage, 12.97 ± 5.12 % amlodipine can be detected by decay in the liver of the corpse by spectrophotometry in the UV region of the spectrum and 12.38 ± 4.68 % by extraction photometry. After 30 days, it is possible to detect amlodipine only when using spectrophotometry in the UV region of the spectrum – 6.40 ± 6.97 % of the substance, and after 40 days, it is impossible to detect amlodipine in the liver of the corpse when it rot.                                                            Conclusions. Based on the results of the research, an algorithm for directional analysis of amlodipine in putrefactive biological material was developed, suitable for forensic toxicological examination, and the term of storage of the test substance was studied. It was established that after 30 days, amlodipine can be detected only when spectrophotometry is used in the UV region of the spectrum – 6.40 ± 6.97 % of the substance; after 40 days, it is impossible to detect amlodipine in the liver of a corpse when it is rotting. The developed methods can be proposed for introduction into the practice of the Bureau of Forensic Science, toxicological centers.


Keywords


amlodipine besylate; biological material in its putrefactive decomposition; the term of storage

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