Foundation mixing technology of cells and mushrooms C. albicans and C. tropicalis
DOI:
https://doi.org/10.24959/ubphj.17.98Keywords:
candidiasis, antigen, vaccine, mix, technologyAbstract
Topicality. Number of patients with candidiasis grow every year, and for its treatment doctors the same antimycotics for many years, the agent gradually loses sensitivity for them. Nowadays abroad, actively conducted research to develop vaccines against candidiasis.
Aim. Tojustify the technology mixing cell fungi C. albicans and C. tropicalis.
Materials and methods. For the ultrasonic mixing used ultrasonic intensity setting at 0.3, 0.5 and 0.7 W/cm2and exposure 5, 10 and 15 min. For the mechanical mixing used the device using mixing with an electric mixer with a speed of 50, 70 and 100 rev/min for 5, 10 and 15 min. After having finished the mixing process, 1 ml of the suspension was selected in each case, which was diluted in 106 and plated on medium Hissa in the same volume of 1 ml.
Results and discussion. The obtained results were showed that the use of ultrasound in investigating parameters have not provide uniform mixing cell fungi C. albicans and C. tropicalis. The using of mixer at rotation speed of 100 t/min for 15 min exposure amount CFU mushrooms C. albicans and C. tropicalis was almost identical, that is indicated a complete and uniform mixing of cells of fungi.
Conclusions. The result of experiment has found the using of mixer with speed of 100 rev/min for 15 min in 100 ml t/min by mixing 50 ml suspension of cell C. albicans fungi and 50 ml suspension of cell mushrooms C. tropicalis provides
the best results.
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