A comparative study of carboxylic acids of the bog bilberry overground parts


  • O. O. Stremoukhov National University of Pharmacy, Ukraine
  • O. M. Koshovyi National University of Pharmacy, Ukraine
  • N. V. Borodina National University of Pharmacy, Ukraine
  • O. V. Kryvoruchko National University of Pharmacy, Ukraine https://orcid.org/0000-0002-3106-0204




bog bilberry (Vaccinium uliginosum), carboxylic acids, chromatography-mass spectrometry


Topicality. Among the small-fruit crops bog bilberry (Vaccinium uliginosum L.) of the heather family (Ericaceae) is becoming increasingly popular. It is cultivated in Ukraine as a fruit, medicinal and ornamental plant. Previously simple
phenols, hydroxycinnamic acids, flavonoids and tannins of this plant leaves were studied.
Aim. To study carboxylic acids qualitative composition and quantitative content of bog bilberry overground parts.
Materials and methods. Leaves, stems and fruits of bog bilberry were collected in 2016 at the “Sadko” Garden Center in Kyiv region. Determination of carboxylic acids in raw material was carried out using an Agilent Technologies 6890N chromatograph with a mass spectrometric detector 5973N.
Results and discussion. Using chromatography-mass spectrometry the content of 37 carboxylic acids has been determined in bog bilberry raw material studied; among them there are 17 fatty acids (13 saturated and 4 unsaturated acids), 9 aromatic acids, 2 hydroxy acids, 8 dibasic acids and 1 keto acid. The following acids prevail in all the objects of the study: among fatty acids – palmitic acid, among hydroxy acids – citric one, among aromatic acids – benzoic and vanillic acids, and among dibasic acids – malonic and oxalic ones. p-Hydroxycinnamic acid was identified only in stems of bog bilberry, dodecacarbonyl acid – in leaves, octadicarboxylic acid – in fruits, ferulic acid – in leaves and fruits of the plant.
Conclusions. The content of 37 carboxylic acids in leaves, stems and fruits has been determined by chromatography-mass spectrometry. The total amount of carboxylic acids identified in leaves of bog bilberry is 5867.84 mg/kg, in stems – 7918.61 mg/kg, in fruits – 23833.4 mg/kg. Further pharmacognostic study of the bog bilberry overground parts is promising for creating new drugs on their basis.


Konovalova, O. Yu., Mitchenko, F. A., Shuraieva, T. K. (2008). Biolohichno aktyvni rechovyny likarskykh roslyn. Kyiv: Kyivskyi universytet, 280.

Lee, B.–L., Kang, J.–H., Kim, H.–M., Jeong, S.–H., Jang, D.–S., Jang, Y.–P., Choung, S.–Y. (2016). Polyphenol–enriched Vaccinium uliginosum L. fractions

reduce retinal damage induced by blue light in A2E–laden ARPE19 cell cultures and mice. Nutrition Research, 36 (12), 1402–1414. doi: 10.1016/j.


Kiseleva, T. L. (2007). Lechebnye svoistva pishchevykh rastenii. Moscow: FNKETcTMDL Roszdrava, 533.

Gubanov I. A. (2004). Illiustrirovannyi opredelitel rastenii Srednei Rossii. V 3 t., T. 3. Pokrytosemennye (dvudolnye: razdelnolepestnye). Moscow:

KMK, In–t tekhnolog. issled., 25.

Stremoukhov, O. O. (2016). Tovaroznavchyi analiz tovariv obmezhenoho aptechnoho asortymentu. Kharkiv: NUPh, 19.

Stremoukhov, O. O., Peshkova, O. S., Koshovyi, O. M., Kireiev, I. V. (2016). Farmatsiia XXI stolittia, tendentsii ta perspektyvy, 1, 141.

Stremoukhov, A. A., Koshevoi, O. N. (2016). Farmatsevtychna nauka ta praktyka: problemy, dosiagnennia, perspektyvy rozvytku, 139.

Heffels, P., Müller, L., Schieber, A., Weber, F. (2017). Profiling of iridoid glycosides in Vaccinium species by UHPLC–MS. Food Research International,

, 462–468. doi: 10.1016/j.foodres.2016.11.018





Pharmaceutical chemistry and pharmacognosy