Determination of chloropyramine in biological material by method of derivative spectrophotometry

O. O. Mamina, V. I. Kabachny, S. G. Nikolaychuk, Yu. V. Kovtyuh

Abstract


Topicality. Studies of biological objects in the forensic toxicology departments of the forensic medical examination bureau are conducted in the absence of control experiments. The presence of impurities in the extracts from the biological
material reduces the objectivity of the results obtained in the quantitative determination of substances by spectrophotometry in the UV region of the spectrum. The use of purification methods (chromatography in a thin layer of sorbent (TLC), extraction with various organic solvents) does not allow to completely isolate coextractive substances from the extracts. The choice of the method of derivative spectrophotometry for the identification and quantitative determination of drugs in the chemical-toxicological analysis makes it possible to eliminate the influence of the impurity background.
Aim. To study the elaboration of method for the determination of chloropyramine in biological material using a derivative spectrophotometry suitable for forensic toxicological examination.
Materials and methods. For the study, solutions of chloropyramine hydrochloride in water, ethanol, 0.1 M solution of chloride acid, 0.01 M sodium hydroxide solution, as well as model mixtures of liver tissue of animal origin and the
preparation were used. Chloropyramine was isolated from the biological material with water acidified with oxalic acid, purification was carried out by extraction of impurities with hexane at pH 2.0 and TLC-method. The content of the substance
was determined by spectrophotometry in the UV region of the spectrum and the derivative of spectrophotometry.
Results and discussion. Identification and quantitative determination of chloropyramine hydrochloride in solutions in water, ethanol, 0.1 M solution of chloride acid, 0.01 M sodium hydroxide solution was carried out using the
second derivative of the light absorption spectra, which was calculated by polynomial approximation method of least squares. Calculations were carried out using differential digital filters. When analyzing the preparation in a biological
material, it was established that the spectral characteristics of the substance and biogenic impurities are approximated by polynomials of various degrees, which made it possible to eliminate the influence of the impurity background on the
results of the study. A comparative evaluation of the methods of derivative spectrophotometry and spectrophotometry in the UV region of the spectrum for the quantitative determination of chloropyramine in extracts from biological material was carried out. It is established that the method of derivative spectrophotometry makes it possible to obtain reliable and objective results in the absence of control samples.                                                                Conclusions. Based on the results of the research, a method for the identification and quantitative determination of chloropyramine in a biological material was developed using a derivative spectrophotometry suitable for forensic toxicological examination. The possibility of using the method of derivative spectrophotometry to correct the effect of coextractive substances on the results of biological objects analysis was shown. When comparing the methods of derivative spectrophotometry and spectrophotometry in the UV region of the spectrum, it was established that both methods didn’t not have significant discrepancies, therefore practically equivalent. In the absence of control samples and impurity background influence, the method of derivative spectrophotometry made it possible to obtain reliable and objective results.


Keywords


chloropyramine hydrochloride; biological material; the method of derivative spectrophotometry

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DOI: https://doi.org/10.24959/ubphj.18.159

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