Study of flavonoids composition and hypoglicemic effect of dry extracts of phaseolus vulgaris pods




phaseolus vulgaris pods, dry extract, flavonoids, hypoglycemic effect


Topicality. Combination of synthetic hypoglycemic agents with herbal medicines characterized by complex effects on different systems of the body, low toxicity and no adverse reactions, is appropriate for ensuring effective pharmacological correction in type 2 diabetes.

Aim. To study flavonoids composition and their content in phaseolus vulgaris pods dry extracts, to research a hypoglycemic action.

Materials and methods. The chromatographic and spectrophotometric methods were used while studying flavonoids. Experiments to study the hypoglycemic activity were performed on white non-linear rats by performing a “glucose load” test.

Results and discussion. The routine, isoquercitin and ferulic acid were chosen as markers for identification of the phaseolus vulgaris pods dry extracts according to the results of chromatographic studies of it. The content of flavonoids in the extract rises from 0.562 to 0.729 % with an increase of alcohol content (from 40 to 70 % (v/v)) in the extractant used to make the extract. Application of the investigated extracts causes the most intense reduction of glucose level in blood of The experimental groups animals in relative to the control group level by 27.9-20.0 % for 30-90 minutes after glucose loading.

Conclusions. The active markers for identifying of the phaseolus vulgaris pods dry extracts are proposed. The content of flavonoids rises from 0.562 to 0.729 % with an increase of ethyl alcohol content from 40 to 70 % (v/v). The greatest decrease of glucose in the blood of experimental animals is observed with the use of extracts obtained by extractants with an alcohol content of 40 % and 50 %.

Author Biographies

L. V. Vronska, I. Horbachevsky Ternopil State Medical University

Cand. of Chem. Sci., Associate Professor Department of Pharmacy, Postgraduate Education Institute

A. I. Dub, I. Horbachevsky Ternopil State Medical University

Master of Pharmacy, the search engineer of the Department of Management and Economics of Pharmacy with the technology of medicines

I. M. Klishch, I. Horbachevsky Ternopil State Medical University

Doctor of Biol. Sci., Professor of the Department of Functional and Laboratory Diagnostics, Vice-Rector for Research

A. Ye. Demyd, I. Horbachevsky Ternopil State Medical University

Cand. of Chem. Sci., Associate Professor, Department of General Chemistry


1. IDF Diabetes Atlas, 7–th edition. (2015).Brussels,Belgium: International Diabetes Federation, 144. Availble at:

Chaudhury, A., Duvoor, C., Reddy Dendi, V. S., Kraleti, S., Chada, A., Ravilla, R., Mirza, W. (2017). Clinical Review of Antidiabetic Drugs: Implications for Type 2 Diabetes Mellitus Management. Frontiers in Endocrinology, 8. doi: 10.3389/fendo.2017.00006

Yeh, G. Y., Eisenberg, D. M., Kaptchuk, T. J., Phillips, R. S. (2003). Systematic Review of Herbs and Dietary Supplements for Glycemic Control in Diabetes. Diabetes Care, 26 (4), 1277–1294. doi: 10.2337/diacare.26.4.1277

Derymedvid, L. V., Bukhtiiarova, I.P. (2011). Farmakolohiia i likarska toksykolohiia, 2 (21), 37–42.

Kinichenko, A. O., Klevanova, V. S., Trzhetsynskyi, S. D., Maletskyi, M. M. (2017). Farmatsevtychnyi zhurnal, 2, 68.

Ezhned, M. A., Hroshovyi, T. A., Horoshko, O. M. (2015). Farmatsevtychnyi chasopys, 3, 63–65.

Rybak, V. A., Maloshtan, L. M. (2013). Ukrainskyi biofarmatsevtychnyi zhurnal, 6 (29), 42–45.

Tundis, R., Loizzo, M. R., Menichini, F. (2010). Natural Products as α–Amylase and α–Glucosidase Inhibitors and their Hypoglycaemic Potential in the Treatment of Diabetes: An Update. Mini–Reviews in Medicinal Chemistry, 10 (4), 315–331. doi: 10.2174/138955710791331007

Rybak, V. A., Kryvoruchko, O. V., Maloshtan, L. M., Samoilova, V. A., Kovalov, V. M. (2014). Likuvalno–profilaktychnyi zasib iz hipohlikemichnoiu diieiu z lystia kyzylu. Pat. 89735 Ukraine , MPK A61K 36/40 (2006.01), A61P 3/10 (2006.1); declared 16.12.2013; published 25.04.2014, № 8.

Kurylo, Kh. I., Klishch, I.M. (2017). Medychna ta klinichna khimiia, 19 (3), 35–41.

11. Derzhavnyi reiestr likarskykh zasobiv Ukrainy. Available at:

Kohlmünzer, S. (2007). Farmakognozja. Warszawa, 595.

Kovalov, V. M., Kovalov, S. V., Demeshko, O. V., Dmytriievskyi, D. I., Kutsanian, A. S., Maloshtan, L. M. (2015). Sposib oderzhannia kompleksu biolohichno aktyvnykh rechovyn z hipohlikemichnoiu diieiu. Pat. 101850 Ukraine, MPK A61K 36/48 (2006.01), A61P 3/10 (2006.1); declared 08.12.2014; published 12.10.2015, № 19.

Oonsivilai R., Manatwiyangkool, J., Oonsivilai, A. (2011) Extraction Condition of Phaseolus vulgaris. International Journal of Nutrition and Food Engineering, 12 (12), 869–872.

Barrett, M. L., Udani, J. K. (2011). A proprietary alpha–amylase inhibitor from white bean (Phaseolus vulgaris): A review of clinical studies on weight loss and glycemic control. Nutrition Journal, 10 (1). doi: 10.1186/1475–2891–10–24

Onakpoya, I., Aldaas, S., Terry, R., Ernst, E. (2011). The efficacy of Phaseolus vulgaris as a weight–loss supplement: a systematic review and meta–analysis of randomised clinical trials. British Journal of Nutrition, 106 (02), 196–202. doi: 10.1017/s0007114511001516

Rybak, V. A., Maloshtan, L. M. (2014). Farmakolohiia ta likarska toksykolohiia, 4–5, 80–84.

Vronska, L. V. (2013). Farmatsevtychnyi chasopys, 4, 82–87.

Vronska, L. V. (2015). ScienceRise, 12/4 (17), 47–53.

Vronska, L.V., Chubka, M. B., Demyd, A. Ye. (2015). Development of phenolic compounds chromatographic identification in bilberry shoots. Farmatsevtychnyi chasopys, 3, 28–33. doi: 10.11603/2312–0967.2015.3.4951

21. Derzhavna Farmakopeia Ukrainy: v 3 t. (2015). Kharkiv: Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv, 1, 1128.

22. Medychna statystyka. Availble at:

Luca, C. D., Olatunde, A., Tijjani, H., Olisa–Enewe, I.A. (2013). Effect of aqueous extract of Phaseolus Vulgaris L. (Red Kidney Beans) on alloxan–induced diabetic wistar rats. Int. J. of Science Invention Today, 2 (4), 292–301.

Santimone, M., Koukiekolo, R., Moreau, Y., Le Berre, V., Rougé, P., Marchis–Mouren, G., Desseaux, V. (2004). Porcine pancreatic α–amylase inhibition by the kidney bean (Phaseolus vulgaris) inhibitor (α–AI1) and structural changes in the α–amylase inhibitor complex. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics, 1696 (2), 181–190. doi: 10.1016/j.bbapap.2003.11.001

Ishimoto, M., Suzuki, K., Iwanaga, M., Kikuchi, F., Kitamura, K. (1995). Variation of seed α–amylase inhibitors in the common bean. Theoretical and Applied Genetics, 90 (3–4). doi: 10.1007/bf00221985

Antoniuk, V .O. (2005). Lektyny ta yikh syrovynni dzherela. Lviv: Kvart, 554.

Kochubei, T. O., Maksymchuk, O. V., Matsevych, L. L. et al. (2014). Ukrainskyi biokhimichnyi zhurnal, 86 (4), 103–109.





Pharmaceutical chemistry and pharmacognosy