Study of flavonoids composition and hypoglicemic effect of dry extracts of phaseolus vulgaris pods
Keywords:phaseolus vulgaris pods, dry extract, flavonoids, hypoglycemic effect
Topicality. Combination of synthetic hypoglycemic agents with herbal medicines characterized by complex effects on different systems of the body, low toxicity and no adverse reactions, is appropriate for ensuring effective pharmacological correction in type 2 diabetes.
Aim. To study flavonoids composition and their content in phaseolus vulgaris pods dry extracts, to research a hypoglycemic action.
Materials and methods. The chromatographic and spectrophotometric methods were used while studying flavonoids. Experiments to study the hypoglycemic activity were performed on white non-linear rats by performing a “glucose load” test.
Results and discussion. The routine, isoquercitin and ferulic acid were chosen as markers for identification of the phaseolus vulgaris pods dry extracts according to the results of chromatographic studies of it. The content of flavonoids in the extract rises from 0.562 to 0.729 % with an increase of alcohol content (from 40 to 70 % (v/v)) in the extractant used to make the extract. Application of the investigated extracts causes the most intense reduction of glucose level in blood of The experimental groups animals in relative to the control group level by 27.9-20.0 % for 30-90 minutes after glucose loading.
Conclusions. The active markers for identifying of the phaseolus vulgaris pods dry extracts are proposed. The content of flavonoids rises from 0.562 to 0.729 % with an increase of ethyl alcohol content from 40 to 70 % (v/v). The greatest decrease of glucose in the blood of experimental animals is observed with the use of extracts obtained by extractants with an alcohol content of 40 % and 50 %.
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