Effects of eplerenone on behavioral responses and indicators of carbonyl/oxidative stress in rats with experimental myocardial damage

V. A. Tkachenko

Abstract


Topicality. The degree of ischemic myocardial damage largely depends on the activity of antioxidant protection, therefore antioxidant agents are used for treatment. In cardiology, aldosterone antagonists are used, as an adjunct to standard therapy to reduce the risk of adverse cardiovascular events, among which special popularity is gained by inspra. Data on the antioxidant properties of this drug are single and very contradictory.
Aim. To study the effect of inspra on indicators of carbonyl/oxidative stress and antioxidant system in rats with experimental myocardial damage.
Materials and methods. Myocardial damage was induced in rats by combined administrations of pituitrin and isoproterenol (PIMD) for two days. Animals were divided into four groups (n = 10): 1 – control group; 2 – PIMD-group; 3 – rats were injected with corvitin (C) for 5 days after PIMD, 4 – administered inspra (I) for 5 days after PIMD. Behavioral reactions of rats were examined by open field test. The indicators of carbonyl/oxidative stress, levels of thiobarbituric acid reactive substances (TBARS), glycated end products (AGEs), oxidative modification of proteins (OMP), were investigated in plasma. The activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GP), and glutathione reductase (GR) were analyzed in plasma and red blood cells.
Results and discussion. In PIMD-group changes of physiological and biochemical indicators characteristic of the ischemic state were observed. The levels of TBARS, AGEs, and OMP in the rats blood was significantly increased. The activity of catalase in the blood decreased by 2.2 times, and the level of GP increased. The activity of other enzymes did not change significantly. After treatment of C and I significant changes in the studied parameters were determined: heart rate and ECG dynamics returned to normal, the levels of TBARS, OMB and AGEs were reduced to almost the initial values.
Conclusions. The cardiotherapeutic effect of inspra is partly due to its antioxidant properties, which were more pronounced than after exposure to the classical antioxidant corvitin.


Keywords


inspra; carbonyl/oxidative stress; antioxidant system; experimental myocardial damage

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DOI: https://doi.org/10.24959/ubphj.18.164

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