Screening of Bearberry leaves extracts hypoglycemic effect and study of acute toxicity




Bearberry (Arctostaphylos uva-ursi), acute toxicity, hypoglycemic activity, oral glucose tolerance test


Diabetes mellitus type 2 (DM2) is a global health problem. The plant origin medicinal preparations revealed different mechanisms of antidiabetic action. Despite a wide range of plants that have already been studied, other species are of interest. Particularly, Bearberry (Arctostaphylos uva-ursi) attracted our attention because its leaves are rich with biologically active compounds, but hypoglycemic activity has not been studied yet.

The aim of our experiment was studding acute toxicity and hypoglycemic activity of Bearberry extracts on non-diabetic standard models.

Materials and methods. It was conducted the screening of hypoglycemic effect in healthy rats, oral glucose tolerance test in healthy rats and study of acute toxicity. The object of study were water and alcohol polyphenol extracts of Bearberry leaves (extractor –alcohol 50 % and 90 %).

Results and discussion. Screening and comparative study shows that the maximal hypoglycemic activity revealed Bearberry leaves alcohol extract (extractor – 50 % ethanol, PE50) in dose 100 mg/kg. The least pronounced effect was observed for the introduction of water extract administration.

Conclusions. The results indicate the practicability of this Bearberry leaves extract further study for renewal the range of medicinal products that revealed hypoglycemic activity.

Author Biographies

G. B. Kravchenko, National University of Pharmacy

PhD, Associated Professor of the Biological Chemistry department

Matar Mazen, National University of Pharmacy

post-graduate student of the Biological Chemistry department

O. A. Krasilnikova, National University of Pharmacy

PhD, Associated Professor of the Biological Chemistry department


Dattatreya, A., Sarangi, T. K. (2015). A Review on Diabetes Mellitus: Complications, Management and Treatment Modalities. Research & Reviews: Journal of Medical and Health Sciences, 4 (3), 10. Available at:–access/diabetic–complications––their–treatment.php

Chaudhury, A., Duvoor, C., Reddy Dendi, V. S., Kraleti, S., Chada, A., Ravilla, R., Mirza, W. (2017). Clinical Review of Antidiabetic Drugs: Implications for Type 2 Diabetes Mellitus Management. Frontiers in Endocrinology, 8. doi: 10.3389/fendo.2017.00006

Governa, P., Baini, G., Borgonetti, V., Cettolin, G., Giachetti, D., Magnano, A., Biagi, M. (2018). Phytotherapy in the Management of Diabetes: A Review. Molecules, 23 (1), 105. doi: 10.3390/molecules23010105

Eddouks, M., Bidi, A., El Bouhali, B., Hajji, L., Zeggwagh, N. A. (2014). Antidiabetic plants improving insulin sensitivity. Journal of Pharmacy and Pharmacology, 66 (9), 1197–1214. doi: 10.1111/jphp.12243

Meresa, A., Gemechu, W., Basha, H., Fekadu, N., Teka, F., Ashebir, R., Tadele, A. (2017). Herbal Medicines for the Management of Diabetic Mellitus in Ethiopiaand Eretriaincluding their Phytochemical Constituents. American Journal of Advanced Drug Delivery, 05 (01). doi: 10.21767/2321–547x.1000011

Stefanov, O. V. (2001). Doklіnichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsena, 528.

de Oliveira, D. T., Souza–Silva, E., Scand, T. (2009). Gingival Vein Punction: A New Simple Technique for Drug Administration or Blood Sampling in Rats and Mice: Journal of Laboratory Animal Science, 36 (2), 109–113.

Test No. 423: Acute Oral toxicity – Acute Toxic Class Method. (2012). OECD, 1–14. doi: 10.1787/9789264071001–en






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