Experimental justification of safety of thick extract with spinate garden
Keywords:acute toxicity, spinach extract from garden, mass ratios
Topicality. An important stage of the preclinical study of new drugs is the study of their safety, namely the establishment of a toxicity class in the conditions of short-term use of high doses.
The aim of this study was to determine the acute toxicity of the thick extract from spinach leaves of the garden, which will allow us to assess the breadth of its therapeutic effect.
Materials and methods. The study was carried out on 24 white mongrel rats of both sexes weighing 180-200 grams. The rats were divided into 4 groups, each of which included 6 animals. When choosing doses for intragastric administration, the maximum dose of the fourth toxicity class (low toxicity substances) - 5000 mg / kg, which was chosen for single intragastric administration to rats of males and females of thick extract from spinach from garden, is the limiting factor in the determination of LD50.Control animals were given drinking water. The animals were observed for 14 days and their overall condition, lethality, body mass dynamics (on days 3, 7 and 14) were assessed, and after the experiment, a macroscopic examination of the internal organs was performed and their mass was determined.
Results and its discussion. It was found that after intragastric administration of a thick extract of spinach from garden vegetables at a dose of 5000 mg / kg, no signs of intoxication were found in the experimental animals, no lethality was found in the experimental groups of animals. When analyzing the body weight of animals, there was no toxic effect of the thick extract from spinach on this indicator. During the entire follow-up period, a physiological increase in the body weight of the experimental animals occurred, which was reliable with respect to the initial data at the end of the experiment.
Analysis of the data of mass coefficients of internal organs of rats of males and females after single intragastric administration of an extract from spinach and garden animals testifies to the absence of pathological changes in the functional state of experimental animals in comparison with control animals.
Conclusions. The studies conducted to study the acute toxicity of thick extract from the leaves of spinach garden established its lack of toxic effect. According to the toxicological classification of K. Sidorov's substances, the dry extract from spinach leaves (LD 50> 5000 mg / kg) with intragastric administration refers to the V class of toxicity - practically harmless substances.
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