Melatonin and N-acetylcysteine influence on the lipoperoxidation intensity in blood serum and central nervous system of rats with type 1 diabetes mellitus
Keywords:experimental diabetes mellitus, fatty acids, brain, antioxidants, N-acetylcysteine, melatonin
Topicality. Diabetic encephalopathy has a significant medical and social value, since it is one of the most common complications of diabetes mellitus. Medicines with antioxidative properties play an important role in pathogenetic therapy of diabetic encephalopathy.
Aim. To investigate the effect of melatonin and N-acetylcysteine on the lipoperoxidation intensity in blood serum and central nervous system of rats with streptozotocin – induced diabetes mellitus type 1 (DM1).
Materials and methods. MDA and SOD were studied in homogenate of brain tissues and blood serum of rats (with DM1 received physiological solution; rats with DM1 received per os: N-acetylcysteine at a dose of 1.5 g/kg – (NAC), melatonin – 10 mg/kg (Mel) and combination therapy (NAC + Mel); healthy rats (control group)). Fatty acid composition was determined by gas-liquid chromatography.
Results and discussion. We observed the growth of MDA, reduction of SOD in homogenate of brain tissues and growth of MDA in blood serum of rats with DM comparison groups. Administration of NAC and Mel was contributed to the reduction of MDA concentration and normalization of SOD level in homogenate of brain tissues. Administration of Mel was contributed to the reduction of MDA concentration and growing of SOD in serum blood also. Pharmacotherapy NAC and Mel was caused normalization of the fatty acids in the brain of rats with diabetes type 1: the levels of palmitic and linoleum acids were increased, and the level of arachidonic acid was decreased. Mel increased the level of oleic acid also. NAC in mono- and combination therapy contributed to the improvement of the fatty acid composition in blood serum`s lipids of rats with diabetes mellitus type 1 by reducing the amount of saturated and increasing unsaturated and polyunsaturated fatty acids. Mel also increased the content of unsaturated fatty acids in blood serum.
Conclusions. Therapy of NAC and Mel caused to the decreasing in the intensity of lipoperoxidation in the blood serum and central nervous system of rats with streptozotocin – induced type 1 diabetes mellitus.
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