Study of dynamic of extraction BAS from motherwort herb for obtaining the tincture

Ye. A. Romanenko, O. M. Koshovyi, A. M. Kovalyova, T. V. Ilyina, A. M. Komissarenko

Abstract


Topicality. Tincture of Motherwort herb is one of the most popular herbal remedies of sedative activity. Tincture is obtained by maceration in the ratio of 1: 5. In the waste of the Motherwort herb there is still a significant amount of BAS. Taking this into account, it is advisable to investigate the dynamics of BAS extraction from the Motherwort herb and determine its optimal multiplicity.

The aim of the study. Study of the dynamics of extraction of BAS from Motherwort herb by 70% ethanol to determine the optimal multiplicity in obtaining tincture from this raw material.

Materials and methods. Determination of derivatives of hydroxycinnamic acid, flavonoids and henolic compounds was performed by spectrophotometric method and HPLC method. In order to optimize the extraction of BAS and to select the optimal extraction multiplicity, the coefficient of mass return of each stage (mi extract/miethanol) for each of the indicators was calculated, the dependence of these factors on the extraction multiplicity to determine the optimal extraction multiplicity was deduced from the application package.

Results and discussions. As a result of the HPLC analysis of the extracts from Motherwort herb, 5 substances were identified: 2 flavonoids (routine and catechin) and 3 hydrocinnamic acids (chlorogenic, coffeic and rosemarinic), and their quantitative content was determined. In the largest number of extracted extracts contained chlorogenic acid and routine.

In the obtained extracts from the ethanol by spectrophotometric method, the content of the main groups of BAS was determined: hydroxycinnamic acids, flavonoids and the sum of phenolic compounds.

According to the results of the research, the equation of dependence of the profitability of alcohol extraction from Motherwort herb on the extraction of the extractives, the amount of phenolic compounds, hydroxycholic acids, flavonoids and corresponding graphs were constructed.

Conclusions. The study of qualitative composition and quantitative content of the main groups of BAR (flavonoids, hydroxycinnamic acids) in extracts from Motherwort herb and using mathematical methods showed that the optimal multiplicity of extraction of BAS from this raw material for the manufacture of tincture is twice.


Keywords


Motherwort; herb; tincture; extraction; optimization

References


Koshovyi, O. M., Romanenko, Ye. A., Komissarenko, A. M. (2016). The study of the phenolic composition of the dry extract of motherwort herb and

its psychotropic activity. American Journal of Science and Technologies, “Princeton University Press”, № 1 (21), 1055–1059.

Derzhavne pidpryiemstvo «Ukrainskyi naukovyi ekspertnyi farmakopeinyi tsentr yakosti likarskykh zasobiv». (2015) Derzhavna farmakopeia

Ukrainy. ( Vols 1–3 vol.1). (2-e ed.) Kharkiv, 1128.

Koshovyi, O. M., Komissarenko, A. M., Kovalyova, A. M., Mudryk, I. M. (2005). Farmacom, 2/3, 151–161.

Kovalyova, A. M., Heorhievskyi, G. V., Kovalyov, V. M., Komisarenko, A. M., Tymchenko, M. M. (2002). Farmacom, 2, 92–97.

Golembiovska, O. I. (2014). Simultaneous determination of flavonoids and phenolic acids in different parts of Prunella vulgaris L. by high-performance

liquid chromatography with photodiode array detection. Int. J. Pharmacog. Phytochem., 29 (1), 1248–1255.

Koshovyi, O. M., Kukhtenko, O. S., Kovalyova, A. M., Komissarenko, A. M., Vinnik, O. V., Sholom, Yu. G. (2010). Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, ХХІІІ (1), 47–49.

Kafarov, V. V. (1976). Metody kibernetiki v khimii i khimicheskoi tekhnologii. Moscow: Khimiia, 464.


GOST Style Citations


1. Koshovyi, O. The study of the phenolic composition of the dry extract of motherwort herb and its psychotropic activity / O. Koshovyi, Y. Romanenko,
A. Komissarenko // Am. J. of Sci. and Technol., “Princeton University Press”. – 2016. – № 1 (21). – P. 1055–1059.

2. Державна фармакопея України : в 3 т. / Державне підприємство «Український науковий фармакопейний центр якості лікарських засобів». – 2-е вид. – Х.: Державне підприємство «Український науковий фармакопейний центр якості лікарських засобів». – 2015. – Т. 1. – 1128 с.

3. Дослідження фенольних сполук листя евкаліпту / О. М. Кошовий, А. М. Комісаренко, А. М. Ковальова та ін. // Фармаком. – 2005. –
№ 2/3.– С. 151–161.

4. Розробка метода стандартизації нового лікарського засобу піфламін / А. М. Ковальова, Г. В. Георгієвський, В. М. Ковальов та ін. // Фармаком. – 2002. – № 2. – С. 92–97.

5. Golembiovska, O. I. Simultaneous determination of flavonoids and phenolic acids in different parts of Prunella vulgaris L. by high-performance
liquid chromatography with photodiode array detection // Int. J. Pharmacog. Phytochem. – 2014. – Vol. 29 (1). – P. 1248–1255.

6. Оптимізація процесу екстракції біологічно активних речовин листя евкаліпта: кратність екстракції / О. М. Кошовий, О. С. Кухтенко,
А. М. Ковальова та ін. // Акт. питання фарм. і мед. науки та практики. – 2010. – Вип. ХХІІІ, № 1. – С. 47–49.

7. Кафаров, В. В. Методы кибернетики в химии и химической технологии / В. В. Кафаров. – М. : Химия, 1976. – 464 с.





DOI: https://doi.org/10.24959/ubphj.18.185

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