DOI: https://doi.org/10.24959/ubphj.19.200

Effect of three-day Glutathione introduction on hydrogen sulfide metabolism in liver of rats under experimental nephropathy conditions

E. O. Ferenchuk, I. V. Gerush

Abstract


Topicality. Scientists are becoming more interested in the exchange of sulfur-containing amino acids and hydrogen sulfide (H2S) metabolism and glutathione. But the effect of tripeptide on the H2S metabolism by nephropathy is not studied enough.

Aim. To study the effect of tree-day glutathione introduction on the system of H2S-producing enzymes, concentration and production in the liver of rats under conditions of experimental nephropathy.

Materials and methods. The experiment was conducted on albino mature male rats. The animals in experimental group were administered a single intraperitoneal dose of folic acid (250 mg/kg). Glutathione was introduced intragastral (100 mg/kg) during 3 days after intoxication.

Results and discussion. Under conditions of experimental nephropathy, there was a decrease in concentration of hydrogen sulfide by 37.92 % and the production of hydrogen sulfide by 34.84 % compared with the control group. The introduction of glutathione led to an increase in these indicators by 36.32 % and 48.4 % compared with the animals with nephropathy. The activity of cystathionine-γ-lyase (CSE), cystathionine-β-synthase (CBS) and cysteinaminotransferase (CAT) in the liver of rats with nephropathy was reduced by 38.98 %, 33.73 % and 26.76 % compared with animal control group. Glutathione increased the activity of CSE and CBS by 31 % and 33.55 % compared with a group of animals with nephropathy. The activity of cystathionine aminotransferase in the conditions of tripeptide increased by 49.3 % introduction and exceeded the date of the control group.

Conclusions. The activity of H2S-producing enzymes in experimental nephropathy in the liver of rats is decreased. The introduction of glutathione increased the content of hydrogen sulfide and promoted the growth of the activity of H2S-producing enzymes in the liver of rats with nephropathy. As reasons for this effect, the possibility of including tripeptide as a source of cysteine in the synthesis of hydrogen sulfide is considered.

 


Keywords


nephropathy; hydrogen sulfide; glutathione

References


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GOST Style Citations


1. Dominko, K. Glutathionylation: A regulatory role of glutathione in physiological processes / K. Dominko, D. Dikic // Arch. Ind. Hyg. Toxicol. – 2018. – Vol. 69. – P. 1–24. https://doi.org/10.2478/aiht-2018-69-2966

2. Giuffre, A. Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology / A. Giuffre, J. B. Vicente // Oxid. Med. Cell. Longev. – 2018. – Vol. 2018. – P. 1–31. https://doi.org/10.1155/2018/6290931

3. Kimura, Y. Hydrogen Sulfide Increases Glutathione Production and Suppresses Oxidative Stress in Mitochondria / Y. Kimura, Y.–I. Goto // Antioxidants & Redox Signaling. – 2010. – № 12 (1). – Р. 1–13.

4. Folic acid induces acute renal failure (ARF) by enhancing renal prooxidant state / A. Gupta, V. Puri, R. Sharma, S. Puri // Experimental and Toxicol. Pathol. – 2012. – Vol. 64 (3). – P. 225–232. https://doi.org/10.1016/j.etp.2010.08.010

5. Stipanuk, M. H. Characterization of the enzymic capacity for cysteine desulphhydration in liver and kidney of the rat / M. H. Stipanuk, P. W. Beck // Biochem. J. – 1982. – Vol. 206 (2). – P. 267–277. https://doi.org/10.1042/bj2060267

6. The vasorelaxant effect of H2S as a novel endogenous KATP channel opener / W. Zhao, J. Zhang, Y. Lu, R. Wang // Eur. Molecular Biol. Organization. – 2001. – Vol. 20. – P. 6008–6016. https://doi.org/10.1093/emboj/20.21.6008

7. Siegel, L. M. A direct microdetermination for sulfide / L. M. Siegel // Analytical Biochem. – 1965. – Vol. 11. – P. 126–132. https://doi.org/10.1016/0003-2697(65)90051-5

8. Protein measurement with the folin phenol reagent / O. H. Lowry, N. I. Rosenbrougn, A. L. Farr, R. I. Randall // J. Biol. Chem. – 1951. – Vol. 193. – P. 265–275.

9. Allen, J. Effects of oral glutathione supplementation on systemic oxidative stress biomarkers in human volunteers / J. Allen, R. D. Bradley // J. Altern. Complement Med. – 2011. – Vol. 17 (9). – P. 827–833. https://doi.org/10.1089/acm.2010.0716

10. Hydrogen sulfide mediates the vasoactivity of garlic / G. A. Benavides, G. L. Squadrito, R. W. Mills et al. // Proc. Natl. Acad. Sci. USA. – 2007. – Vol. 104. – P. 17977–17982. https://doi.org/10.1073/pnas.0705710104




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Abbreviated key title: Ukr. bìofarm. ž.

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