Study of flavonoids and hydroxycinnamic acids of the Dahlia genus grass cultivated in Ukraine
Keywords:Dahlia, grass, phenolic compounds, flavonoids, hydroxycinnamic acids
Topicality. Phenolic compounds are a group of biologically active compounds, which have anti-inflammatory, antimicrobial, antioxidant, and other types of action. As well known from the literature, phenolic compounds are accumulated in dahlia leaves and flowers, but Dahlia cultivars’ grass chemical composition have not been examined in Ukraine yet.
Aim. To determine the qualitative composition and quantitative content of the amount of hydroxycinnamic acids and the amount of flavonoids in grass of common cultivars of the Dahlia genus in Ukraine, such as: Smuglyanka, Svirel, and Spoloh.
Materials and methods. The objects of the research were the Dahlia genus grass: Smuglyanka, Svirel, and Spoloh. Identification of phenolic compounds was performed using paper chromatography; quantitative determination was performed by the method of spectrophotometry in terms of chlorogenic acid (sum of hydroxycinnamic acids) and rutin (sum of flavonoids).
Results and discussion. Phenolic substances were found by qualitative reactions in all objects. According to the results of chromatographic analysis carried out, 9 substances were found in Smuglyanka cultivar, 6 of which were identified; 10 substances were found in Svirel cultivar, 9 of which were identified; 11 substances were found in Spoloh cultivar, 8 of which were identified. Among hydroxycinnamic acids in all cultivars, ferulic, chlorogenic, and neoclorogenic acids were identified. Among the flavonoids in all cultivars, rutin, quercetin, and kaempferol were identified. The quantitative content of the sum of hydroxycinnamic acids (0.78 %) and the sum of flavonoids (0.91 %) dominated in Svirel cultivar.
Conclusions. For the first time, the qualitative composition and quantitative content of hydroxycinnamic acids and flavonoids in Dahlia cultivars Smuglyanka, Svireli, and Spoloh have been studied. Among hydroxycinnamic acids in all cultivars, ferulic, chlorogenic, and neoclorogenic acids were identified. Among flavonoids in all cultivars, routine, quercetin, and campherol were identified. The quantitative content of the sum of hydroxycinnamic acids and flavonoids dominated in Svirel cultivar. The content of these substances was 0.78 % and 0.91 %, respectively.
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