The experimental study of the «Arthritan» drug influence on the induced and spontaneous pain reactions

Authors

DOI:

https://doi.org/10.24959/ubphj.20.253

Keywords:

“Arthritan”, analgetic activity, induced pain, spontaneous pain

Abstract

Topicality. Over the past decades, traditional medicines, in particular herbal preparations, have become increasingly widespread worldwide, due to their multivector pharmacodynamics, a high enough level of safety and accessibility to a wide population.
Aim. To conduct a preclinical trials to study the analgesic properties of the drug “Arthritan” and phytocomposition based on it.
Materials and methods. The study of analgesic properties was carried out under conditions of the development of inflammatory hyperalgesia in 50 rats of both sexes weighing 150-180 g, during which the intensity of the pain reaction was determined according to the Randall-Selitto method. The influence of the studied objects on the course of spontaneous pain response in 40 rats was evaluated in the conditions of acute arthritis in the knee joint.
Results and discussion. In the study of analgesic properties in a model of induced pain response in rats with inflammatory hyperalgesia, the combination of “Arthritan”, “Nevrin” and “Nephrolik” showed a high level of analgesic activity, amounted to 30.5 %, significantly exceeded the activity of other drugs. The analgesic activity of the “Arthritan” and “Nevrin” preparations was 20.8 % and 15.9 %, respectively; the “Nephrolik” preparation did not have a significant analgesic effect. Under conditions of the development of a spontaneous pain reaction in rats with acute gonarthritis, the studied phytocomposition showed a high level of analgesic effect with repeated administrations, the activity of which  n the 5th day of the experiment was 41.9 %, which significantly exceeds all other means. The preparations “Arthritan” and “Nevrin” showed activity – 19.1 % and 17.9 %, respectively, and the drug “Nephrolic” – 7.8 %, which is an unreliable result. The high level of analgesic activity of the studied phytocomposition is due to the potentiating interaction of its components such as “Arthritan” and “Nevrin”.
Conclusions. The results of the studies allow us to recommend further research of the combined preparation containing “Arthritan”, “Nevrin” and “Nephrolik” at a ratio of 1 : 1 : 0.5 as a means of chondroprotective, anti-inflammatory and analgesic action in rheumatological desease profile patients.

Author Biographies

Yu. Naboka, National University of Pharmacy

postgraduate student, of the Department of Clinical Pharmacology and Clinical Pharmacy

N. Zubitska, LLC “Zemna´s Green Planet”

General Director

I. Zupanets, National University of Pharmacy

Doctor of Medicine, Professor, Head of the Department of Clinical Pharmacology and Clinical Pharmacy

S. Shebeko, National University of Pharmacy

PhD in Pharmacy, Associate professor of the Department of Clinical Pharmacology and Clinical Pharmacy

I. Otrishko, National University of Pharmacy

PhD in Pharmacy, Associate professor of the Department of Clinical Pharmacology and Clinical Pharmacy

References

Harna, S. V., Vladymyrova, I. M., Burd, N. B., Heorhiiants, V. A., Kotov, A. H., Prokopenko, T. S., Vasylieva, O. A. (2016). Suchasna fitoterapiia navchalnyi posibnyk. Kharkiv, 580.

Harnyk, T. P., Tumanov, V. A., Pokanevych, V. V., Frolov, V., Peresadin, M. (2012). Fitoterapiia. Chasopys, 1, 4–11.

Bone, K., Mills, S. (2012). Principles and Practice of Phytotherapy : Modern Herbal Medicine. Hardbound, 1056.

Heinrich, M. (2012). Fundamentals of Pharmacognosy and Phytotherapy. UK : Elsevier Health Sciences, 336.

Kunle, O. (2012). Standardization of herbal medicines – A review. International Journal of Biodiversity and Conservation, 4 (3), 101–112. https://doi.org/10.5897/ijbc11.163

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. (2010). Official Journal of the European Union, L. 276, 33–79.

Guide for the care and use of laboratory animals (8th ed.). (2011). Washington : The National Academies Press.

Randall, L. O., Selitto, J. J. (1957). A method for measurement of analgesic activity on inflamed tissue. Arch. Int. Pharmacodyn, 111 (4), 409–419.

Vogel, H. G. (ed.). (2008). Drug discovery and evaluation: pharmacological assays. Berlin: Springer-Verlag, 2071.

Neugebauer, V., Han, J. S., Adwanikar, H., Fu, Y., & Ji, G. (2007). Techniques for Assessing Knee Joint Pain in Arthritis. Molecular Pain, 3, 1744–8069–3–8. https://doi.org/10.1186/1744-8069-3-8

Stephanov, A. V. (2002). Doklinicheskie issledovaniia lekarstvennyh sredstv. Kiev: Avitcena, 528.

Islam, M. A., Al-Shiha, A. (2018). Foundations of Biostatistics. Singapore: Springer, 474. https://doi.org/10.1007/978-981-10-8627-4

Published

2020-03-02

Issue

Section

Pharmacology and biochemistry