Effect of piridoxin on the skin reparation process under experimental insulin resistance
Keywords:pyridoxine hydrochloride, insulin resistance, wound healing, antiglycosylation properties
Topicality. According to WHO experts estimation, the worldwide prevalence of diabetes mellitus is pandemic, with more than 80 % of patients dying from the fatal effects of diabetic angiopathy. One of the most common complications of diabetes of both types is the formation of chronic wound defects, and eventually gangrene of the lower limbs with the transformation into sepsis, which cases to 3.2 % of deaths against the background of diabetes.
Aim. To study the effect of combined medicine magnesium and pyridoxine on the course of the reparative skin process in rats with experimental insulin resistance.
Materials and methods. The study has been carried out in 60 rats males weighing 180-220 g. Experimental insulin resistance in rats was induced under conditions of the hypercaloric diet and intraperitoneal administration of dexamethasone. Studies of the process of skin repair have been performed on models of full-layer stencil and linear cut wounds, as well as determination of carbohydrate metabolism markers and glycosylation products in serum and hemolysate.
Results and discussion. The use of pyridoxine in combination with a reparant (retinol acetate) resulted in a significant reduction in wound area as early as 5 day of treatment and effectively reduced the total time of complete epithelialization; and also increased the load under tensiometry, which corresponded to 72.8 % of the reparative activity of this therapeutic scheme. Against the background of the pyridoxine the content of fructosamine and glycosylated hemoglobin also has been significantly reduced, which confirms the antiglycosylative properties of vitamin.
Conclusions. Pyridoxine is able to potentiate the wound healing effect of reparants under the insulin resistance due to its antiglycosylation properties. The results of this study allow us to recommend further research of pyridoxine in combination with standard skin repairs in the treatment of wounds in patients with hyperglycemia and diabetes mellitus.
Xu, L., Kanasaki, K., Kitada, M., Koya, D. (2012). Diabetic angiopathy and angiogenic defects. Fibrogenesis Tissue Repair, 5 (1), 13. https://doi.org/10.1186/1755-1536-5-13
Yazdanpanah, L., Shahbazian, H., Nazari, I., Arti, H. R., Ahmadi, F., Mohammadianinejad, S. E., Cheraghian, B., &Hesam, S. (2018). Incidenceandriskfactors of diabeticfootulcer: a population-baseddiabeticfootcohort (adfcstudy)-two-yearfollow-upstudy. Internationaljournal of endocrinology, 7631659. https://doi.org/10.1155/2018/7631659
Zhang, P., Lu, J., Jing, Y., Tang, S., Zhu, D., Bi, Y. (2017). Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis. Annals of Medicine, 49 (2), 106–116. https://doi.org/10.1080/07853890.2016.1231932
Singh, V. P., Bali, A.,Singh, N., Jaggi, A. S. (2014). Advanced glycation end products and diabetic complications. Korean journal of physiology and pharmacology, 18 (1), 1–14. https://doi.org/10.4196/kjpp.2014.18.1.1
Brem, H., Tomic-Canic, M. (2007). Cellular and molecular basis of wound healing in diabetes. Journal of ClinicalInvestigation, 117(5), 1219–1222. https://doi.org/10.1172/JCI32169
Adrover, M., Vilanova, B., Muñoz, F., & Donoso, J. (2005). Inhibition of Glycosylation Processes: the Reaction between Pyridoxamine and Glucose. Chemistry & Biodiversity, 2 (7), 964–975. https://doi.org/10.1002/cbdv.200590074
Higuchi, O., Nakagawa, K., Tsuzuki, T., Suzuki, T., Oikawa, S., & Miyazawa, T. (2006). Aminophospholipid glycation and its inhibitor screening system: a new role of pyridoxal 5′-phosphate as the inhibitor. Journal of Lipid Research, 47 (5), 964–974. https://doi.org/10.1194/jlr.m500348-jlr200
Jahnen-Dechent, W., & Ketteler, M. (2012). Magnesium basics. Clinical Kidney Journal, 5 (Suppl 1), i3–i14. https://doi.org/10.1093/ndtplus/sfr163
Arpaci, D., Tocoglu, A. G., Ergenc, H., Korkmaz, S., Ucar, A., & Tamer, A. (2015). Associations of serum Magnesium levels with diabetes mellitus and diabetic complications. Hippokratia, 19 (2), 153–157.
Barbagallo, M., Dominguez, L. J. (2015). Magnesium and type 2 diabetes. World Journal of Diabetes, 6 (10), 1152–1157. https://doi.org/10.4239/wjd.v6.i10.1152
Kozhem`iakin, Yu. M., Khromov, O. S., Filonenko, M. A., Saifetdynova, H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv : Avitsenna, 156.
Directive (EU) 2010/63/EU of the European Parliament and of the Council of 22 September 2010. (2010). On the Protection of Animals Used for Scientific Purposes. Official Journal of the European Union, 276, 33–79.
Zahaiko, A. L., Briukhanova, T. O., Shkapo, A. I. (2015). Informatsiinyi lyst “Modyfikatsiia metodu modeliuvannia eksperymentalnoi insulinorezystentnosti u shchuriv”. Kyiv, 7.
Mironov, A. N. (Ed.). (2013). Rukovodstvo po provedeniiu doklinicheskikh issledovanii lekarstvennykh sredstv. Chast pervaia. Moscow: Grif i K, 944.
Nair, A., & Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7 (2), 27. https://doi.org/10.4103/0976-0105.177703
Yakovlieva, L. V., Tkachova, O. V., Butko, Ya. O., Lar’ianovska, Yu. B. (2013). Eksperymentalne vyvchennia novykh preparativ dlia mistsevoho likuvannia ran. Metodychni rekomendatsii. Kharkiv: Vyd-vo NFaU, 52.
Rebrova, O. Iu. (2006). Statisticheskii analiz meditcinskikh dannykh. Primenenie paketa programm Statistica. Moscow: MediaSfera, 312.
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).