Effect of piridoxin on the skin reparation process under experimental insulin resistance

D. Lytkin, T. Briukhanova, A. Zagayko, M. Mostovych


Topicality. According to WHO experts estimation, the worldwide prevalence of diabetes mellitus is pandemic, with more than 80 % of patients dying from the fatal effects of diabetic angiopathy. One of the most common complications of diabetes of both types is the formation of chronic wound defects, and eventually gangrene of the lower limbs with the transformation into sepsis, which cases to 3.2 % of deaths against the background of diabetes.

Aim. To study  the effect of combined medicine magnesium and pyridoxine on the course of the reparative skin process in rats with experimental insulin resistance.

Materials and methods. The study has been carried out in 60 rats males weighing 180-220 g. Experimental insulin resistance in rats was induced under conditions of the hypercaloric diet and intraperitoneal administration of dexamethasone. Studies of the process of skin repair have been performed on models of full-layer stencil and linear cut wounds, as well as determination of carbohydrate metabolism markers and glycosylation products in serum and hemolysate.

Results and discussion. The use of pyridoxine in combination with a reparant (retinol acetate) resulted in a significant reduction in wound area as early as 5 day of treatment and effectively reduced the total time of complete epithelialization; and also increased the load under tensiometry, which corresponded to 72.8 % of the reparative activity of this therapeutic scheme. Against the background of the pyridoxine the content of fructosamine and glycosylated hemoglobin also has been significantly reduced, which confirms the antiglycosylative properties of vitamin.

Conclusions. Pyridoxine is able to potentiate the wound healing effect of reparants under the insulin resistance due to its antiglycosylation properties. The results of this study allow us to recommend further research of pyridoxine in combination with standard skin repairs in the treatment of wounds in patients with hyperglycemia and diabetes mellitus.


pyridoxine hydrochloride; insulin resistance; wound healing; antiglycosylation properties


Xu, L., Kanasaki, K., Kitada, M., Koya, D. (2012). Diabetic angiopathy and angiogenic defects. Fibrogenesis Tissue Repair, 5 (1), 13.

Yazdanpanah, L., Shahbazian, H., Nazari, I., Arti, H. R., Ahmadi, F., Mohammadianinejad, S. E., Cheraghian, B., &Hesam, S. (2018). Incidenceandriskfactors of diabeticfootulcer: a population-baseddiabeticfootcohort (adfcstudy)-two-yearfollow-upstudy. Internationaljournal of endocrinology, 7631659.

Zhang, P., Lu, J., Jing, Y., Tang, S., Zhu, D., Bi, Y. (2017). Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis. Annals of Medicine, 49 (2), 106–116.

Singh, V. P., Bali, A.,Singh, N., Jaggi, A. S. (2014). Advanced glycation end products and diabetic complications. Korean journal of physiology and pharmacology, 18 (1), 1–14.

Brem, H., Tomic-Canic, M. (2007). Cellular and molecular basis of wound healing in diabetes. Journal of ClinicalInvestigation, 117(5), 1219–1222.

Adrover, M., Vilanova, B., Muñoz, F., & Donoso, J. (2005). Inhibition of Glycosylation Processes: the Reaction between Pyridoxamine and Glucose. Chemistry & Biodiversity, 2 (7), 964–975.

Higuchi, O., Nakagawa, K., Tsuzuki, T., Suzuki, T., Oikawa, S., & Miyazawa, T. (2006). Aminophospholipid glycation and its inhibitor screening system: a new role of pyridoxal 5′-phosphate as the inhibitor. Journal of Lipid Research, 47 (5), 964–974.

Jahnen-Dechent, W., & Ketteler, M. (2012). Magnesium basics. Clinical Kidney Journal, 5 (Suppl 1), i3–i14.

Arpaci, D., Tocoglu, A. G., Ergenc, H., Korkmaz, S., Ucar, A., & Tamer, A. (2015). Associations of serum Magnesium levels with diabetes mellitus and diabetic complications. Hippokratia, 19 (2), 153–157.

Barbagallo, M., Dominguez, L. J. (2015). Magnesium and type 2 diabetes. World Journal of Diabetes, 6 (10), 1152–1157.

Kozhem`iakin, Yu. M., Khromov, O. S., Filonenko, M. A., Saifetdynova, H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv : Avitsenna, 156.

Directive (EU) 2010/63/EU of the European Parliament and of the Council of 22 September 2010. (2010). On the Protection of Animals Used for Scientific Purposes. Official Journal of the European Union, 276, 33–79.

Zahaiko, A. L., Briukhanova, T. O., Shkapo, A. I. (2015). Informatsiinyi lyst “Modyfikatsiia metodu modeliuvannia eksperymentalnoi insulinorezystentnosti u shchuriv”. Kyiv, 7.

Mironov, A. N. (Ed.). (2013). Rukovodstvo po provedeniiu doklinicheskikh issledovanii lekarstvennykh sredstv. Chast pervaia. Moscow: Grif i K, 944.

Nair, A., & Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy, 7 (2), 27.

Yakovlieva, L. V., Tkachova, O. V., Butko, Ya. O., Lar’ianovska, Yu. B. (2013). Eksperymentalne vyvchennia novykh preparativ dlia mistsevoho likuvannia ran. Metodychni rekomendatsii. Kharkiv: Vyd-vo NFaU, 52.

Rebrova, O. Iu. (2006). Statisticheskii analiz meditcinskikh dannykh. Primenenie paketa programm Statistica. Moscow: MediaSfera, 312.

GOST Style Citations

1.    Diabetic angiopathy and angiogenic defects / L. Xu, K. Kanasaki, M. Kitada, D. Koya // Fibrogenesis Tissue Repair. – 2012. – № 5 (1). – P. 13.


2.    Incidence and Risk Factors of Diabetic Foot Ulcer : A Population-Based Diabetic Foot Cohort (ADFC Study)-Two-Year Follow-Up Study / L. Yazdanpanah et al. // International J. of Endocrinol. – 2018. – Article ID:7631659.


3.    Global epidemiology of diabetic foot ulceration : a systematic review and meta-analysis / P. Zhanget et al. // Annals of Medicine. – 2017. – № 49 (2). – P. 106–116.


4.    Advanced Glycation End Products and Diabetic Complications / V. P. Singh, A. Bali, N. Singh, A. S. Jaggi. // Korean J. of Physiol. and Pharmacol. – 2014. – № 18 (1). – P. 1–14.


5.    Brem, H. Cellular and molecular basis of wound healing in diabetes / H. Brem, M. Tomic-Canic // J. of Clin. Investigation. – 2007. – № 117(5). – P. 1219–1222.


6.    Inhibition of Glycosylation Processes : the Reaction between Pyridoxamine and Glucose / M. Adrover, B. Vilanova, F. Muñoz, J. Donoso // Chemistry&Biodiversity. – 2005. – № 2 (7). – P. 964–975.


7.    Aminophospholipid glycation and its inhibitor screening system : a new role of pyridoxal 5′-phosphate as the inhibitor / O. Higuchiet et al. // J. of Lipid Res. – 2006. – № 47 (5). – P. 964–974. 


8.    Jahnen-Dechent, W. Magnesium basics / W. Jahnen-Dechent, M. Ketteler // Clinical Kidney J. – 2012. – № 5 (1). – P. 3–14.


9.    Associations of serum Magnesium levels with diabetes mellitus and diabetic complications / D. Arpaciet et al. // Hippokratia. – 2015. – № 19 (2). – P. 153–157.


10.  Barbagallo, M. Magnesium and type 2 diabetes / M. Barbagallo, L. J. Dominguez // World J. of Diabetes. – 2015. – № 6 (10). – P. 1152–1157. 


11.  Науково-практичні рекомендації з утримання лабораторних тварин та роботи з ними / Ю. М. Кожем`якін, О. С. Хромов, М. А. Філоненко, Г. А. Сайфетдинова. – К. : Авіценна, 2002. – 156 с.


12.  Directive (EU) 2010/63/EU of the European Parliament and of the Council of 22 September 2010. On the Protection of Animals Used for Scientific Purposes. Official Journal of the European Union. – 2010. – № 276. – P. 33–79.


13.  Інформаційний лист «Модифікація методу моделювання експериментальної інсулінорезистентності у щурів» // А. Л. Загайко, Т. О. Брюханова, А. І. Шкапо. – К., 2015. – 7 с.


14.  Руководство по проведению доклинических исследований лекарственных средств. Часть первая / Под ред. А. Н. Миронова. – М. : Гриф и К, 2013. – 944 с.


15.  Nair, A. B. A simple practice guide for dose conversion between animals and human / A. B. Nair, S. Jacob // J. of Basicand Clinical Pharmacy. – 2016. – № 7 (2). – P. 27–31. 


16.  Експериментальне вивчення нових препаратів для місцевого лікування ран: метод. рек. / Л. В. Яковлєва, О. В. Ткачова. Я. О. Бутко, Ю. Б. Лар’яновська. – Х. : Вид-во НФаУ, 2013. – 52 с.


17.  Реброва, О. Ю. Статистический анализ медицинских данных. Применение пакета программ Statistica / О. Ю. Реброва // М.: МедиаСфера 2006. – 312 с.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)