Effect of piridoxin on the skin reparation process under experimental insulin resistance





pyridoxine hydrochloride, insulin resistance, wound healing, antiglycosylation properties


Topicality. According to WHO experts estimation, the worldwide prevalence of diabetes mellitus is pandemic, with more than 80 % of patients dying from the fatal effects of diabetic angiopathy. One of the most common complications of diabetes of both types is the formation of chronic wound defects, and eventually gangrene of the lower limbs with the transformation into sepsis, which cases to 3.2 % of deaths against the background of diabetes.

Aim. To study  the effect of combined medicine magnesium and pyridoxine on the course of the reparative skin process in rats with experimental insulin resistance.

Materials and methods. The study has been carried out in 60 rats males weighing 180-220 g. Experimental insulin resistance in rats was induced under conditions of the hypercaloric diet and intraperitoneal administration of dexamethasone. Studies of the process of skin repair have been performed on models of full-layer stencil and linear cut wounds, as well as determination of carbohydrate metabolism markers and glycosylation products in serum and hemolysate.

Results and discussion. The use of pyridoxine in combination with a reparant (retinol acetate) resulted in a significant reduction in wound area as early as 5 day of treatment and effectively reduced the total time of complete epithelialization; and also increased the load under tensiometry, which corresponded to 72.8 % of the reparative activity of this therapeutic scheme. Against the background of the pyridoxine the content of fructosamine and glycosylated hemoglobin also has been significantly reduced, which confirms the antiglycosylative properties of vitamin.

Conclusions. Pyridoxine is able to potentiate the wound healing effect of reparants under the insulin resistance due to its antiglycosylation properties. The results of this study allow us to recommend further research of pyridoxine in combination with standard skin repairs in the treatment of wounds in patients with hyperglycemia and diabetes mellitus.

Author Biographies

D. Lytkin, National University of Pharmacy

assistant of the biological chemistry department, head’s assistant of ESIAP

T. Briukhanova, National University of Pharmacy

Candidate of Biological Sciences, assistant of the Department of Biological Chemistry

A. Zagayko, National University of Pharmacy

Doctor of Biological Sciences, Professor of the Department of Biological Chemistry, vice-rector

M. Mostovych, National University of Pharmacy

student, Department of Biological Chemistry


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Pharmacology and biochemistry