DOI: https://doi.org/10.24959/ubphj.20.274

Influence of lead intoxication on morphological state of placenta-fertility system in rats in experiment

M. Berezniakova, L. Perekhoda, N. Bereznyakova, L. Karabut

Abstract


Topicality. Currently, one of the urgent issues of the analysis of environmental pollution is the presence of lead compounds with toxic effect on the functional systems of the human body. Regarding the literature data, lead compounds cause morphofunctional changes primarily in pregnant women’s placenta, leed to various disorders in physiological development of the fetus. Therefore, studies of exogenous effect of lead on the reproductive system of the body is a relevant and timely aspect in predicting and preventing its possible effect on pregnancy and the physiological development of the newborn.

Aim. To study the effect of lead to morphological changes in the hematoplacental barrier of rats, morphological disorders of liver and kidney tissues in early postnatal ontogenesis.

Materials and methods. An experimental study has been conducted on 30 female rats of weight 200-250 g and 70 rats on the 2-d, 17th, 30th, 40th day of postnatal development. The first group of rats included 10 females with physiological pregnancy. The second group consisted of 20 females who received daily oral lead acetic acid at a dose of 50 mg/kg body weight.

Results and discussion. The electron microscopic results of experimental studies showed a violation of the compensatory mechanisms of the blood-brain barrier after daily gain of lead acetic acid. Newborn rats obtained by a physiologically healthy pregnancy have in the liver tissues was observed a rearrangement of the embryonic structure, the absence of a lobular and beam structure, a large number of cellular mitoses in the nuclei of hepatocytes. In all the study groups revealed circulatory disorders which manifested in the form of plethora of capillaries, portal and central veins. Erythrocyte hemolysis and areas of marked lymphohistiocytic infiltration of the connective tissue portal tracts were observed.

Conclusions. According to the results of the study, it has been found the introduction of lead acetic acid at a dose of 50 mg/kg per day to pregnant rats adduced to dystrophic and discirculatory disorders in liver and kidneys of their offspring in postnatal ontogenesis, proved the consequence of lead toxicological poisoning.


Keywords


lead intoxication; toxicology; morphology; placental-fetal system

References


Beletskaia, E. N., Onul, N. M., Bezub, O. V. (2014). Medical Prospects, 130 –138.

Dovhal, H. V. 2016. Ukrainskyi morfolohichnyi almanakh, 42–44.

Anju, A. (2012). Toxicity and fate of heavy metals with particular reference to developing foetus. Advances in Life Sciences, 2, 29–38. DOI: 10.5923/j.als.20120202.06.

Haouas, Z., Zidi, I., Hichri, H. et al. (2015). Hepatotoxic effects of lead acetate in rats: histopathological and cytotoxic studies. Journal of Cytology and Histology, 5, 16-23. DOI: 10.4172/2157-7099.1000256.

Hegazy, A., Fouad, U. (2014). Evaluation of Lead Hepatotoxicity; Histological, Histochemical and Ultrastructural Study. Forensic Medicine and Anatomy Research, 3, 70. DOI: 10.4236/fmar.2014.23013.

Vylegzhanina, T. A. (2015). Influence of lead acetate on the development of the liver of rats. Original scientific publications, 44–48.

Trakhtenberg, I. M. (2011). Prophylactic toxicology and medical ecology. Kyiv: Avitsena, 120.

Sernov, L. N., Hatsura, V. V. (2000). Elements of experimental pharmacology. Moscow, 192.

Romanenko, O. A., Dovhal, H. V., Dovhal, M. A. (2017). Immunostasis of the liver in the prenatal period with lead acetate and lead for the correction. News of problems of biology and medicine, 3, 158–161.

Metwally, E., Negm, F., El-din, R., Nabil, E. (2015). Anatomical and Histological Study of the Effect of Lead on Hepatocytes in Albino Rats. International Journal of Biomedical Materials Research, 3, 34–45. DOI: 10.11648/j.ijbmr.20150304.11.

Randa, A., Dawlat, M., Nariman, A. et al. (2015). Clinicopathological, histopathological and immunolog1ical studies on animals exposed to lead and cadmium under experimental conditions. New York Science J., 5, 120–136.

Bahrii, M. M., Dibrova, V. A., Popadynets, O. H., Hryschuk, I. M. (2016). Methods of morphological attainment. Vinnytsia: Nova Knyga, 328.


GOST Style Citations


1.   Белецкая Э. Н., Онул Н. М., Безуб О. В. Гигиенические аспекты остеотропности свинца как фактора риска кальций-дефицитной патологии у человека (обзор литературы). // Медичні перспективи. 2014. С. 130–138.

 

2.   Довгаль Г. В. Морфологічні зміни в розвитку печінки щурів при впливі ацетату свинцю та за умов корекції в пренатальному періоді. Український морфологічний альманах. 2016. С. 42–44.

 

3.   Anju A. Toxicity and fate of heavy metals with particular reference to developing foetus. Advances in Life Sciences. 2012. № 2. P. 29–38. DOI: 10.5923/j.als.20120202.06 (Date of access: 25.06.2020).

 

4.   Hepatotoxic effects of lead acetate in rats: histopathological and cytotoxic studies / Z. Haouas et al. Journal of Cytology and Histology. 2015. № 5. P. 16-23. DOI: 10.4172/2157-7099.1000256 (Date of access: 25.06.2020).

 

5.   Hegazy A., Fouad U. Evaluation of Lead Hepatotoxicity; Histological, Histochemical and Ultrastructural Study. Forensic Medicine and Anatomy Research. 2014. № 3. P. 70. DOI: 10.4236/fmar.2014.23013 (Date of access: 25.06.2020).

 

6.   Вылегжанина Т. А. Влияние ацетата свинца на развитие печени крыс. Оригинальные научные публикации. 2015. С. 44–48.

 

7.   Трахтенберг І. М. Профілактична токсикологія та медична екологія. Київ : Авіценна, 2011. 120 с.

 

8.   Сернов Л. Н., Гацура В. В. Элементы экспериментальной фармакологии. Москва, 2000. С. 192.

 

9.   Романенко О. А., Довгаль Г. В., Довгаль М. А. Імуногістохімічне дослідження печінки щурів у пізньому пренатальному періоді під впливом ацетату свинцю та за умов корекції. Вісник проблем біології і медицини. 2017. № 3. С. 158–161.

 

10. Metwally E., Negm F., El-din R., Nabil E. Anatomical and Histological Study of the Effect of Lead on Hepatocytes in Albino Rats. International Journal of Biomedical Materials Research. 2015. № 3. P. 34–45. DOI: 10.11648/j.ijbmr.20150304.11 (Date of access: 25.06.2020).

 

11. Clinicopathological, histopathological and immunolog1ical studies on animals exposed to lead and cadmium under experimental conditions / A. Randa et al. New York Science J. 2015. № 5. Р. 120–136.

 

12. Багрій М. М., Діброва В. А., Попадинець О. Г., Грищук І. М. Методики морфологічних досліджень : монографія. Вінниця : Нова книга, 2016. 328 с.





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)