Research to substantiate the conditions of obtaining the oil extract of phytocomposition
Keywords:oil extracts, technology, spectrophotometry, carotenoids, chlorophyll
Topicality. Lipophilic extractions (LE) from mediсinal herbs is a promising source of a number of biologically active substances, namely vitamins, pigments, essential oils, etc., which contribute to the detection of a wide range of pharmacological activity: antioxidant, antibacterial, anti-inflammatory, reparative, making them widely used in phytotherapy and cosmetology. While obtaining LE the maximum output of biologically active compounds (BAC) must be provided to have the required pharmacological action.
Aim. To determine the dependence of the output of biologically active substances from the concentration of ethanol, used for humidification of raw materials in obtaining an oil extract from a mixture of medicinal herbal material (MHM).
Materials and methods. Were examined the samples of oil extracts from phytocomposition containing Salviae herba, Eucalypti folia, Calendulae flores, Сhamomillae flores in the ratio (2:1:1:1), obtained by different technology. The extraction efficiency was monitored by the yield of carotenoids and chlorophyll. The quantitative content of these pigments was determined by spectrophotometry method.
Results and discussion. In the course of research it was established that the previous humidification of phytocomposition with ethanol contributes to more complete yield of carotenoids and chlorophyll. It was determined that the maximum output of these BAS occurs after humidification of a mixture of MHM with 70 % ethanol.
Conclusions. According to the results of the study of quantitative content of chlorophylls and carotenoids amount, it has been shown that the humidification of a mixture of MHM with 70 % alcohol solution at a temperature of 25 ± 5 °C increases the yield of BAS from the phytocomposition containing Salviae herba, Eucalypti folia, Calendulae flores, Сhamomillae flores in ratio (2:1:1:1).
Shikov, A. N., Makarov, V. G. Rychenkov, V. Ye. (2004). Rastitelnye masla i maslianye ekstrakty: tekhnolohiia, standartizatsiia, svoistva. Moscow, 100–112.
Barnes, J., Anderson, L., Phillipson, D., Barnes, J. (2007). Herbal Medicines. (3rd ed.). London: PhP, 710.
Trineieva, O. V., Safonova, E. V. (2013). Khimiia rastitelnoho syria, 4, 77–82.
DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. (2014). Derzhavna Farmakopeia Ukrainy. (Vols. 1–3; Vol. 3). (2nd ed.). Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”, 734.
Bondarenko, Zh. V., Likhodiievskii, A. V., Slonskaia S. V. (2017). Proceedings from Pererabotka i upravleniie kachestvom selskokhaziaistvennoi produktsii: sb. statei III Mezhdunarodnoi nauk.-prakt. konf. (23-24 marta 2017 g.). (pp. 383–385). Minsk: BGATU. Available at: https://rep.bsatu.by/handle/doc/928.
Korotkov, V. A., Kukhtenko, O. S., Hladukh, Ye. V. (2013). Farmatsevtychnyi zhurnal, 6, 36–40. Available at: https://pharmj.org.ua/index.php/journal/article/view/360.
Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, 4 (3), 196. doi: 10.4172/2167-0412.1000196.
Gupta, A., Naraniwal, M., Kothari, V. (2012). Modern extraction methods for preparation of bioactive plant extracts. International journal of applied and natural sciences, 1 (1), 8–26.
Tkachuk, O. Yu., Vyshnevska, L. I., Zubchenko, T. M., Bysaha, E. I. (2015). Zb. nauk. prats spivrobit. NMAPO imeni P. L. Shupyka, 24 (4), 311–315. Available at: https://nmapo.edu.ua/zagruzka/zbornikNMAPO24_4.pdf.
Tkachuk, O. Yu. Vyshnevska, L. I., Zubchenko, T. N. (2016). Influence of polar extractants on optimization of bas release from herbal raw material. The Pharma Innovation, 5 (2), 12–14.
Tkachuk, O. Yu., Vyshnevska, L. I., Zubchenko, T. N. (2016).The study of the effect of the critical parameters on the manufacturing process of the oil phytoextract with the hepatoprotective action. News of Pharmacy, 1 (85), 45–49. doi: https://doi.org/10.24959/nphj.16.2102.
Fedorovska, M. I., Polovko, N. P., Kovpak, L. A. (2015). Klinichna farmatsiia, farmakoterapiia ta medychna standartyzatsiia, 3–4, 114–119. Available at: http://clinpharm.meduniv.lviv.ua/FILES/kfftms_3-4_28-29_2015/kfftms_3-4_28-29_2015.pdf.
Kurehian, A. H. (2015). Fundamentalnye issledovaniia, 2, 5166–5172. Available at: http://www.fundamental-research.ru/ru/article/view?id=38175.
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).