Research to substantiate the conditions of obtaining the oil extract of phytocomposition
DOI:
https://doi.org/10.24959/ubphj.20.282Keywords:
oil extracts, technology, spectrophotometry, carotenoids, chlorophyllAbstract
Topicality. Lipophilic extractions (LE) from mediсinal herbs is a promising source of a number of biologically active substances, namely vitamins, pigments, essential oils, etc., which contribute to the detection of a wide range of pharmacological activity: antioxidant, antibacterial, anti-inflammatory, reparative, making them widely used in phytotherapy and cosmetology. While obtaining LE the maximum output of biologically active compounds (BAC) must be provided to have the required pharmacological action.
Aim. To determine the dependence of the output of biologically active substances from the concentration of ethanol, used for humidification of raw materials in obtaining an oil extract from a mixture of medicinal herbal material (MHM).
Materials and methods. Were examined the samples of oil extracts from phytocomposition containing Salviae herba, Eucalypti folia, Calendulae flores, Сhamomillae flores in the ratio (2:1:1:1), obtained by different technology. The extraction efficiency was monitored by the yield of carotenoids and chlorophyll. The quantitative content of these pigments was determined by spectrophotometry method.
Results and discussion. In the course of research it was established that the previous humidification of phytocomposition with ethanol contributes to more complete yield of carotenoids and chlorophyll. It was determined that the maximum output of these BAS occurs after humidification of a mixture of MHM with 70 % ethanol.
Conclusions. According to the results of the study of quantitative content of chlorophylls and carotenoids amount, it has been shown that the humidification of a mixture of MHM with 70 % alcohol solution at a temperature of 25 ± 5 °C increases the yield of BAS from the phytocomposition containing Salviae herba, Eucalypti folia, Calendulae flores, Сhamomillae flores in ratio (2:1:1:1).
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