Research of carbohydrates in Sea Buckwheat raw materials
Keywords:sea buckthorn, carbohydrates, gas chromatography with mass spectrometric detector
Topicality. Currently, for the treatment and prevention of many diseases are widely used drugs made on the basis of herbal drugs. Each year, their range expands and the number increases. The advantage of phytoremedies over synthetic drugs is their mild action and low toxicity. The main source of herbal remedies are medicinal plants of native flora.
Aim. To study the qualitative composition and content of carbohydrates in herbal drugs of the sea buckthorn varieties of Solodka ginka by chromatographic methods.
Materials and methods. The objects of study were fruits and leaves, of sea buckthorn, harvested at the pharmacopoeial site of National University of Pharmacy of the Ministry of Health of Ukraine (2018). The analysis was performed by gas chromatography with mass spectrometric detector (GC/MS).Results and discussion. The results of the analysis indicate that concentration of sorbitol is the highest in the sea buckthorn leaf (40.42 mg/g), followed by glucose (26.58 mg/g), xylose (11.61 mg/g), arabinose (8.77 mg/g), galactose (8.65 mg/g), rhamnose (3.51 mg/g), mannose (2.67 mg/g), mannitol (2.48 mg/g). In the sea buckthorn fruits (40.59 mg/g) are dominated by sorbitol, xylose (26.03 mg/g), arabinose (4.78 mg/g), mannitol (2.51 mg/g), glucose (1.89 mg/g), other sugars found in fruits and leaves are present in smaller quantities.
Konovalova, O. Yu., Mitchenko, F. A., Shuraieva, T. K. (2008). Biolohichno aktyvni rechovyny likarskykh roslyn. Kyiv: Polihraftsentr “Kyivskyi universytet”, 352.
Popova, N. V., Litvinenko, V. I., Kutcanian, A. S. (2016). Lekarstvennye rasteniia mirovoi flory. Kharkov: Dіsa plius, 540.
World Health Organization. (2003). WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants. Geneva, 72. Available at: https://apps.who.int/iris/bitstream/handle/10665/42783/9241546271.pdf?sequence=1&isAllowed=y.
Olennikov, D. N., Tankhaeva, L. M. (2006). Khimiia rastitelnogo syria, 4, 29–33. Available at: https://cyberleninka.ru/article/n/metodika-kolichestvennogo-opredeleniya-gruppovogo-sostava-uglevodnogo-kompleksa-rastitelnyh-obektov.
Chen, Y., Xie, M.‐Y., Wang, Y.‐X., Nie, Sh.‐P., Li, Ch. (2009). Analysis of the monosaccharide composition of purified polysaccharides in Ganoderma atrum by capillary gas chromatography. Phytochem Analysis, 20 (6), 503–510. doi: https://doi.org/10.1002/pca.1153.
Guerrant, G. O., Moss, C. W. (1984). Determination of monosaccharides as aldononitrile, O-methyloxime, alditol, and cyclitol acetate derivatives by gas-chromatography. Analytical Chemistry, 56 (4), 633–638. doi: https://doi.org/10.1021/ac00268a010.
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