Biopreparations: current application prospects

Authors

  • N. Filimonova National University of Pharmacy of the Ministry of Health of Ukraine
  • I. Tishchenko National University of Pharmacy of the Ministry of Health of Ukraine
  • O. Geyderikh National University of Pharmacy of the Ministry of Health of Ukraine
  • E. Pokrishko I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

DOI:

https://doi.org/10.24959/ubphj.20.289

Keywords:

bacteriophages, lytical activity, virulence

Abstract

Topicality. Taking into account the current state of infectious pathology, the emergence of new diseases, changes in biological properties in the already studied pathogens and the burdened course of infectious diseases, as well as taking into account the prevalence and rate of formation of antibiotic resistance, special attention is drawn to the bacteriophages. Control of the prevalence of infectious agents is achieved both through the use of effective antimicrobial agents and immunobiological drugs for prevention purposes.

Aim. To substantiate the perspective use of bacteriophages in creating immunoprophylactic agents.

Materials and methods. Appelman’s method and the method of double series dilutions were used in the research. Reference strains were used as a microbiological model: S. aureus АТСС 25923, Е. соlі АТСС 25922, В. subtilis АТСС 6633, Р. аеrugenosa 126/18, Pr. mirabilis 128/34, S. abony Dick 1,4,5,12, Str. pyogenes Dick-1.

Results and discussion. The conducted researches on studying the virulence of bacteriophages by means of determination of the lytical activity by the method of titration of a bacteriophage in a liquid nutrient medium (Appelman’s method) have established that the virulence of phages is characterized by the ability of directed lysis of morpho-anatomic structure of target bacteria. In addition, the dependence of the level of lithium titers of phages on the tinctorial characteristics of target bacteria was established: Gram-negative bacteria were 2 times less sensitive to the action of phages.

Conclusions. Obtained results of researches proved expediency of application of bacteriophages for immunoprophylactic purposes due to absence of side immunochemical effects.

Author Biographies

N. Filimonova, National University of Pharmacy of the Ministry of Health of Ukraine

Head of the Department of Microbiology, Virology and Immunology, Doctor of Medical Science, Professor

I. Tishchenko, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Biological Sciences (Ph.D.), Associate Professor, the Department of Microbiology, Virology and Immunology

O. Geyderikh, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Medical Sciences (Ph.D.), Associate Professor, the Department of Microbiology, Virology and Immunology

E. Pokrishko, I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

Candidate of Medical Sciences (Ph.D.), Associate Professor, the Department of Microbiology, Virology and Immunology

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Published

2020-12-07

Issue

Section

Microbiological research