DOI: https://doi.org/10.24959/ubphj.20.294

The study of the cardioprotective action of the thick extract of parsnip herb

L. Horiacha, N. Symonenko, L. Galuzinska, O. Shpychak, V. Kyslychenko

Abstract


Topicality. Modern drugs used to treat diseases of the cardiovascular system generally have a number of side effects. In this regard, the problem of developing and studying safe and effective drugs for the use in cardiology becomes relevant for scientists. Pharmacological drugs with the metabolic and cytoprotective action of plant origin are increasingly used for the treatment of cardiovascular diseases; however, their range is quite limited. The latest information on the use of herbal medicines based on the promising types of the medicinal plant raw material of the domestic flora, in particular parsnip (Pastinaca sativa L.) herb, determines the urgency of creating new potential cardioprotectors of plant origin.

Aim. To study amino acids and phenolic compounds in the thick extract of parsnip herb, as well as the cardioprotective activity of this extract.

Materials and methods. The thick extract of parsnip herb (TEPH) was chosen as the study object. The study of the amino acid composition of TEPH was performed by ion exchange liquid chromatography on a T 339 automatic analyzer. Phenolic compounds were studied by high performance liquid chromatography (HPLC). Identification of components was performed by retention time and compliance of UV spectra with standard substances, the quantitative content was determined by calculating the area of peaks on the chromatogram. The study of the effectiveness of the use of the plant substance of TEPH for the correction of disorders in the body of rats under conditions of heart disease with high doses of adrenaline was performed at the premises of the Central Research Laboratory of the NUPh. The experiment was performed on 25 nonlinear white male rats weighing 180-220 g. The drug “Tricardin” LLC “Ternopharm” (Ukraine) was used as a reference drug.

Results and discussion. As a result of the study of the amino acid composition of TEPH, 18 amino acids were identified; their total content was almost 10 % in the extract. Rutin and chlorogenic acid in the amount of 3.37 mg/100 g and 0.41 mg/100 g, respectively, were identified in the extract by HPLC. The results of the study of the effect of the thick extract of parsnip on the integrated indicators of the state of white rats on the model of acute adrenaline myocarditis indicate that when treating animals with TEPH in the doses of 100 mg/kg and 200 mg/kg the heart mass coefficient increased by 1.25 and 1.2 times compared to the group of intact animals, indicating a decrease in the manifestations of exudation compared to animals of the control pathology.

Conclusions. Amino acids and phenolic compounds in TEPH have been studied. As a result, the presence of 18 amino acids has been found; their content in the extract is almost 10 %. The extract contains 3.37 mg/100 g of rutin and 0.41 mg/100 g of chlorogenic acid. The study conducted has confirmed the cardioprotective properties of TEPH, indicating the relevance of further comprehensive physicochemical, pharmacological and preclinical pharmacological studies of this herbal medicine and emphasizing the prospects for its further study, implementation in production and use in the treatment of cardiovascular diseases.


Keywords


thick extract of parsnip herb; amino acids; phenolic compounds; cardioprotective activity

References


Hromovaia, V. F., Shapoval, H. S., Myroniuk, Y. E., Nestiuk, N. V. (2008). Khimiko-farmatsevticheskii zhurnal, 1 (42), 26–29.

Hulaha, O. І., Tashchuk, V. K., Polians’ka, O. S. (2010). Bukovynskyi medychnyi vіsnyk, 2 (54), 122–124.

Sokolov, S. Ya. (2000). Fitoterapiia i fitofarmakolohiia: Rukovodstvo dlia vrachei. Moscow: Meditsinskoe informatsionnoe agentstvo, 976.

Shymorova, Yu. E., Kislichenko, V. S., Horiachaia, L. N. (2020). Norwegian Journal of development of the International Science, 38, 42–45. Available at: http://nor-ijournal.com/wp-content/uploads/2020/09/NJD_38_1.pdf.

Nepomniashchikh, L. M. (1998). Alternativnaia nedostatochnost myshechnykh kletok serdtca pri metabolicheskikh i ishemicheskikh povrezhdeniiakh. Moscow: BEBiM RAMN, 56.

Hofman, B. (2006). Katekholaminy i sredstva, vliiaiushchie na adrenergicheskuiu peredachu. Klinicheskaia farmakologiia po Gudmanu i Gilmanu (Kn. 1). Moscow, 185.

Chuvaev, I. V., Hlotova, S. V. (2009). Proceeding from Effektivnye i bezopasnye lekarstvennye sredstva v veterinarii: materialy Vseros. sezda veterinarnykh farmakologov i toksikologov. (pp. 28–31). Sait-Petersburg.

Stefanov, O. V. (Ed.). (2001). Doklіnіchnі doslіdzhennia lіkarskyh zasobіv. Kyiv: Avitsenna, 528.

Shchokina, K. H., Shtryhol, S. Yu., Belіk, H. V., Butko, Ya. O. (2010). Ukrainskyi bіofarmatsevtychnyi zhurnal, 2 (7), 39-43.

Khalafian, A. A. (2007). STATISTICA 6. Statisticheskii analiz dannykh. (3rd ed.). Moscow: Binom-Press, 512.

Syrovaia, A. O. et al. (2015). Aminokisloty glazami khimikov, farmatcevtov, biologov. (Vols. 1-2; Vol. 2). Kharkov: Shchedra sadiba plius, 268.

Oleinik, S. A., Horchakova, N. A., Koval, I. V. (2006). Sportyvna medytsyna, 1, 90–99.

Goszcz, K., Duthie, G. G, Stewart, D. (2017). Bioactive polyphenols and cardiovascular disease: chemical antagonists, pharmacological agents or xenobiotics that drive an adaptive response, British Journal of Pharmacology, 174, 1209–1225. doi: https://doi.org/10.1111/bph.13708.

Ciumărnean, L., Milaciu, M. V., Runcan, O. (2020). The Effects of Flavonoids in Cardiovascular Diseases Molecules, 25, 4320. doi: 10.3390/molecules25184320.

Gross, M. (2004) Flavonoids and Cardiovascular Disease. Pharmaceutical Biology., 42, 21–35.

Comana, V., Vodnar, D. C. (2019). Hydroxycinnamic acids and human health: recent advances. Journal of the Science of Food and Agriculture, 100 (2), 483–499. doi:10.1002/jsfa.10010.


GOST Style Citations


1.    Антиоксидантные свойства лекарственных растений / В. Ф. Громовая и др. Химико-фармацевтический журнал. 2008. Т. 42, № 1. С. 26–29. DOI: https://doi.org/10.30906/0023-1134-2008-42-1-26-29.

 

2.    Гулага О. І., Тащук В. К., Полянська О. С. Процеси ліпопероксидації у хворих на серцеву недостатність. Буковинський медичний вісник. 2010. Т. 14, № 2 (54). С. 122–124.

 

3.    Соколов С. Я. Фитотерапия и фитофармакология : Руководство для врачей. Москва : Медицинское информационное агентство, 2000. 976 с.

 

4.    Шиморова Ю. Е., Кисличенко В. С., Горячая Л. Н. Изучение аминокислотного состава пастернака посевного (Pastinaca sativa L.). Norwegian Journal of development of the International Science. 2020. № 38 (1). С. 42–45. URL: http://nor-ijournal.com/wp-content/uploads/2020/09/NJD_38_1.pdf.

 

5.    Непомнящих Л. М. Альтернативная недостаточность мышечных клеток сердца при метаболических и ишемических повреждениях. Москва : БЭБиМ РАМН, 1998. С. 56.

 

6.    Гофман Б. Катехоламины и средства, влияющие на адренергическую передачу. Клиническая фармакология по Гудману и Гилману. Кн. 1. Москва, 2006. С. 185.

 

7.    Чуваев И. В., Глотова С. В. Оптимизация адреналиновой модели инфаркта миокарда на крысах. Эффективные и безопасные лекарственные средства в ветеринарии : материалы Всерос. съезда ветеринарных фармакологов и токсикологов. Санкт-Петербург, 2009. С. 28–31.

 

8.    Доклінічні дослідження лікарських засобів : метод. рек. / за ред. О. В. Стефанова. Київ : Авіценна, 2001. 528 с.

 

9.    Експериментальне вивчення кардіопротекторної активності рекомбінантного антагоніста рецепторів інтерлейкіну-1 (АРІЛ-1) та ліпіну на моделі іммобілізаційного стресу в щурів / К. Г. Щокіна та ін. Український біофармацевтичний журнал. 2010. № 2 (7). С. 39–43.

 

10.  Халафян А. А. STATISTICA 6. Статистический анализ данных. 3-е изд., перераб. и доп. Москва : Бином-Пресс, 2007. 512 с.

 

11.  Аминокислоты глазами химиков, фармацевтов, биологов : в 2 т. / А. О. Сыровая и др. Харьков : Щедра садиба плюс, 2015. Т. 2. 268 с.

 

12.  Препараты аминокислот и их производных в спортивной медицине: реалии и перспективы. 3. Дипептиды, олиго- и полипептиды / С. А. Олейник и др. Спортивна медицина. 2006. № 1. С. 90–99.

 

13.  Bioactive polyphenols and cardiovascular disease: chemical antagonists, pharmacological agents or xenobiotics that drive an adaptive response? / K. Goszcz et al. British Journal of Pharmacology. 2017. Vol. 174, Iss. 11. Р. 1209–1225. DOI: https://doi.org/10.1111/bph.13708 .

 

14.  The Effects of Flavonoids in Cardiovascular Diseases / L. Ciumărnean et al. Molecules. 2020. Vol. 25, Iss. 18. Р. 4320. DOI: https://doi.org/10.3390/molecules25184320.

 

15.  Gross M. Flavonoids and Cardiovascular Disease. Pharmaceutical Biology. 2004. Vol. 42. P. 21–35. DOI: https://doi.org/10.3109/13880200490893483.

 

16.  Coman V., Vodnar D. C. Hydroxycinnamic acids and human health : recent advances. Journal of the Science of Food and Agriculture. 2019. Vol. 100, Iss. 2. P. 483–499. DOI: https://doi.org/10.1002/jsfa.10010.





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)