Experimental study of gluquamin effect on the kidney ultrastructure in conditions of renal failure development

Authors

DOI:

https://doi.org/10.24959/ubphj.18.193

Keywords:

gluquamin, renal failure, nephroprotective action, kidney ultrastructure

Abstract

Topicality. The main clinical manifestation of chronic kidney disease (CKD) is renal failure. Among the most sensitive manifestations of this pathology should be a violation of  renal tissue ultrastructural organization . Therefore, in the experimental study of CKD treatments, it is reasonable to study their effect on the ultrastructure of the kidneys in the conditions of the development of renal failure.

Aim. The experimental study of Gluquamin drug effect on kidney ultrastructure in rats with renal failure to substantiate its use in  CKD treatment.

Materials and methods. The study was carried out on the active Heymann nephritis model using 50 rats. To assess the efficiency of the studied drugs on the 120th day of the experiment, the examination of the kidney ultrastructure was carried out in animals using standard methods of electron microscopy.

Results and discussion. In course of the study, it was shown that after administration the Gluquamin for two months, in rats with renal failure, there was a significant improvement of the ultrastructural organization of the kidneys. At the same time, there were an increase in survival rate of nephrocytes in conditions of pathologically changed kidney, a decrease in manifestations of destruction in glomerular basement membranes, an improve in structure of organelles and metabolism of podocytes and endothelial cells and a decrease in manifestations of degenerative changes in them. According to the degree of nephroprotective influence, Gluquamin was superior to the activity of the comparative drugs quercetin and lespephril.

Conclusions. Under conditions of renal failure development in rats Gluquamin has a pronounced protective effect on kidneys tissue ultrastructure, which confirms the expediency of its use in complex CKD treatment.

Author Biographies

S. Shebeko, National University of Pharmacy

Associate Professor of the Department of Clinical Pharmacology and Clinical Pharmacy

I. Zupanets, National University of Pharmacy

Doctor of Medicine, Professor, Head of the Department of Clinical Pharmacology and Clinical Pharmacy

O. Tarasenko, National University of Pharmacy

PhD in Medicine, Associate Professor of the Department of Clinical Pharmacology and Clinical Pharmacy

References

Pyroh, L. A., Ivanov, D. D. (eds). (2014). Nefrolohiia: natsionalnyi pidruchnyk. Donetsk: Vydavets Zaslavskyi, 292.

Skvortcov, V. V., Tumarenko, A. V. (2017). Klinicheskaia nefrologiia: kratkii kurs. St. Petersburg: SpetcLit, 199.

Shebeko, S. K. (2017). Comparative experimental study of nephroprotective properties of glucosamine derivatives in combination with quercetin. Ukraïns’kij bìofarmacevtičnij žurnal, 5 (52), 40–44. https://doi.org/10.24959/ubphj.17.139

Shebeko, S. K., Zupanets, I. A., Shalamai, A. S. (2017). Pharmacology and Drug Toxicology, 6, 66–71.

Shebeko, S. K., Zupanets, I. A., Propisnova, V. V., & Shalamay, A. S. (2018). Experimental study of Gluquamine efficacy in the case of tubular kidney damage. Ukraïns’kij bìofarmacevtičnij žurnal, 2, 56–60. https://doi.org/10.24959/ubphj.18.170

Shebeko, S. K., Zupanets, I. A., & Shalamay, A. S. (2018). Study of glucuamine specific action under conditions of diabetic nephropathy in the experiment. Ukraïns’kij bìofarmacevtičnij žurnal, 1 (54), 25–30. https://doi.org/10.24959/ubphj.18.155

Guide for the care and use of laboratory animals, 8th edition. (2011). Washington : The National Academies Press, 246.

European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe (1986). Strasbourg, 52.

Shtryhol, S. Yu., Lisovyi, V. M., Zupanets, I. A. (2009). Metody eksperymentalnogo modeliuvannia urazhennia nyrok dlia farmakologichnykh doslidzhen. Kharkiv: NFaU, 48.

Shebeko, S. K. (2017). Liky Ukrainy plus, 4, 26–29.

Stefanov, O. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: “Avitsena”, 528.

Sarkisov, D. S., Perov, Yu. L. (1996). Mikroskopicheskaia tekhnika. Moscow: Meditsina, 544.

Shebeko, S. K., Zupanets, I. A., Shalamai, A. S. (2017). Pharmacology and Drug Toxicology, 6, 66–71.

Downloads

Published

2018-12-05

Issue

No. 4(57) (2018)

Section

Pharmacology and biochemistry

License

Copyright (c) 2018 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).