DOI: https://doi.org/10.24959/ubphj.18.194

Study of the drug “Arthritan” acute toxicity

Yu. Naboka, N. Zubitska, I. Zupanets, S. Shebeko, I. Otrishko

Abstract


Topicality. To ensure the modern herbal medicine integration into the healthcare system is one of the tasks for modern scientific and practical medical community. Therefore, today the actual problem in medical and pharmaceutical industry is  production of high-quality plant origin products in order to meet the needs of  our country population.

Aim. Experimental study of drug “Arthritan” toxicological properties in the context of acute toxicity modeling to justify the safety use in clinical practice.

Materials and methods. The study was performed according to the method of V. B. Prozorovsky with intragastric administration in rats with the dose range of 1.0-5.0 ml/kg. Throughout the study (14 days), observations were made in survival of experimental animals, manifestations of intoxication, and the dynamics of the individual symptoms development.

Results and discussion. Under the conditions of single intragastric administration in rats, the drug “Arthritan” in the dose range of 1.0-5.0 ml/kg did not have a toxic effect on the general condition and  animals behavior, did not cause their death. This allowed us to assume that with this route of administration, the LD50 of the investigational drug exceeds 5.0 ml/kg. The obtained LD50 values allow it to be attributed to the studied route of administration according to the generally accepted classification of K.K. Sidorov to the V toxicity class – practically non-toxic substances.

Conclusions. According the toxicological characteristics «Arthritan» drug is practically non-toxic to the human body, which determines the prospects for its further study with the aim of application in clinical practice.


Keywords


“Artritan”; acute toxicity; rats; safety of use

References


Serhyenko, O. M., Zhyhunova, A. K. (2013). Ukrayinskij medichnij chasopis, 1, 77–80.

Harnyk, T. P., Tumanov, V. A., Pokanevych, V. V., Frolov, V. M., Peresadin, M. O. (2012). Fitoterapiya. Chasopis, 1, 4–11.

Kunle, O. F., Egharevba, H. O., Ahmadu, P. O. (2012). Standardization of herbal medicines - A review. International Journal of Biodiversity and Conservation, 4 (3), 101–112. https://doi.org/10.5897/ijbc11.163

Guide for the care and use of laboratory animals, 8th edition. (2011). Washington : The National Academies Press, 246.

European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe (1986). Strasbourg, 52.

Prozorovskii, V. B. (1992). Prakticheskoe posobie po uskorennomu opredeleniiu srednikh effektivnykh doz i kontcentratcii biologicheski aktivnykh veshchestv. St. Peterburg, 42.

Prozorovskiy, V. B. (2007). Psikhofarmakologiya i biologicheskaya narkologiya, 7 (3-4), 2090–2120.

Stefanov, O. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: «Avitsena», 528.

Sidorov, K. K. (1973). O klassifikatsii toksichnosti yadov pri parenteralnykh sposobakh vvedeniya. – V kn.: Toksikologiya novykh promyshlennykh khimicheskikh veshchestv. Moskva, 13, 47–57.

Truhacheva, N. V. (2012). Matematicheskaia statistika v mediko–biologicheskih issledovaniiah s primeneniem paketa Statistica. Moscow: GEOTAR–Media, 379.


GOST Style Citations


1. Сергиенко, О. М. Роль и место фитотерапии в лечении состояний, сопровождающихся продуктивным кашлем / О. М. Сергиенко, А. К. Жигунова // Укр. мед. часопис. – 2013. – № 1. – С. 77–80.

2. Фітотерапія: сучасні тенденції до використання в лікарській практиці та перспективи подальшого розвитку (огляд літератури та результати власних досліджень) / Т. П. Гарник, В. А. Туманов, В. В. Поканевич та ін. // Фітотерапія. Часопис. – 2012. – № 1. – С. 4–11.

3. Kunle, O. F. Standardization of herbal medicines: A review / O. F. Kunle, H. O. Egharevba, P. O. Ahmadu // Int. J. Biodivers. Conserv. – 2012. – Vol. 4 (3). – P. 101–112. https://doi.org/10.5897/ijbc11.163

4. Guide for the care and use of laboratory animals. – 8-th ed. – Washington : The National Academies Press, 2011. – 246 p.

5. European convention for the protection of vertebrate animals used for experimental and other scientific purpose : Council of Europe. – Strasbourg, 1986. – 52 p.

6. Прозоровский, В. Б. Практическое пособие по ускоренному определению средних эффективных доз и концентрации биологически активных веществ. – С.Пб, 1992. – 42 с.

7. Прозоровский, В. Б. Статистическая обработка результатов фармакологических исследований / В. Б. Прозоровский // Психофармакол. и биол. наркол. – 2007. – Т. 7, № 3-4. – С. 2090–2120.

8. Доклинические исследования лекарственных средств : метод. рек. / под ред. А. В. Стефанова. – К. : Авиценна, 2002. – 528 с.

9. Сидоров, К. К. О классификации токсичности ядов при парентеральных способах введения. – В кн.: Токсикология новых промышленных химических веществ. – М., 1973. – Вып. 13. – С. 47–57.

10. Трухачева, Н. В. Математическая статистика в медико–биологических исследованиях с применением пакета Statistica / Н. В. Трухачева. – М. : ГЭОТАР–Медиа, 2012. – 379 с.





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