Study of phenolic compounds in the Feverfew Herb by TLC and HPLC methods
Keywords:feverfew, herb, Aster family, thin layer chromatography, high performance liquid chromatography, flavonoids, hydroxycinnamic acids
Topicality. The search of new plant sources of biologically active substances is an actual issue for the pharmaceutical science. The feverfew (Tanacetum parthenium (L.) Schultz Bip) is one of the prospective species of the Aster family. Its chemical composition mainly consists of phenolic compounds and sesquiterpene lactones. According to the latest data of the foreign sources feverfew herb phenolic compounds stipulate a prominent anti-inflammatory effect. This species is successfully used to treat the chronic inflammatory diseases of the connective tissue. Therefore, the determination of the qualitative composition and quantitative content in the samples of domestic feverfew raw material is actual.
Aim. To determinate the qualitative composition and quantitative content of phenolic compounds in the feverfew herb.
Materials and methods. The object was the feverfew herb collected in July 2017 on the territory of the Botanical garden of the National University of Pharmacy. The qualitative composition and quantitative content were determinated by the thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) methods.
Results and discussion. Chlorogenic and cichoric acids zones, luteolin and luteolin7-glucoside in comparison with
their zones of reference standards were identified by TLC method. 12 compounds were identified and determinated by HPLC method. Hydroxycinnamic acids, namely 3,5-dicaffeoylquinic acid (1.575 %), 4,5-dicaffeoylquinic acid (1.308 %) and chlorogenic acid (0.784 %) were accumulated in the feverfew herb in the greatest amount. Among flavonoids, apigenin-7-glucoside (0.071 %) and kaempferol (0.041 %) prevailed.
Conclusions. For the first time the qualitative composition and quantitative content in the feverfew herb were determinated by TLC and HPLC methods. The high content of the phenolic compounds in the plant raw material attests to the opportunity of feverfew herb standardization by such classes as hydroxycinnamic acids and flavonoids. The data obtained attest to the perspective creating a medicine with the high anti inflammatory activity based on the feverfew herb.
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