Determination of conditionally therapeutic dose of dry extract from Salat Crop leaves on a model of tetrachlormethane lesion of liver in rats

Authors

DOI:

https://doi.org/10.24959/ubphj.19.227

Keywords:

dry extract from lettuce, tetrachloromethane hepatitis, white rats

Abstract

Topicality. The search for natural remedies that would exhibit antiseptic, anti-inflammatory, detoxification, antioxidant, choleretic, hepatoprotective actions and positively influenced the entire body in order to create new medicines based on them is one of the pressing issues of modern pharmacology and pharmacy. Plants used for food can serve as a basis for new medicines.
Aim. To establish a conditionally therapeutic dose of the extract from lettuce leaves in the experiment with simulated tetrachloromethane hepatitis in rats.
Materials and methods. The experiments were carried out on white outbred male rats, who were simulated toxic liver damage with carbon tetrachloride (twice (every other day) at a dose of 1.0 ml/kg body weight). Euthanasia was performed using sodium thiopental on the fourth day after the introduction of carbon tetrachloride in compliance with all rules for working with vertebrate animals. For the study, extract doses of 50 mg, 100 mg, 150 mg and 200 mg per kilogram of body weight of the animal were used. The activity of lipid peroxidation was assessed by the content of TBAactive products, the activity of membrane-destructive processes - by the activity of aminotransferases (AsAT and AlAT), the state of the antioxidant system by the content of CP and catalase activity.
Results and discussion. It was noted that the lesion of rats with carbon tetrachloride causes a significant (p ≤ 0.05)
increase in serum levels of TBA-active products (2.1 times), ceruloplasmin (5.6 times) and aminotransferase activities. At the same time, a decrease in catalase activity is observed (by 5.8 times). The activity of AsAT and AlAT in the serum during this period increases almost equally – by 4 times. Significant changes in the studied parameters observed after application of a dose of 100 mg / kg of body weight of animals.
Conclusions. A conditionally therapeutic dose of the extract is considered to be a dose of 100 mg/kg of body
weight, which has a positive effect on the indices of lipid peroxidation, antioxidant system and cytolysis under conditions of modeled toxic hepatitis.

Author Biographies

V. P. Pyda, I. Horbachevsky Ternopil National Medical University

Candidate of Pharmacy, Associate professor of the Department of Pharmacology with Clinical Pharmacology, I. Horbachevsky Ternopil

L. S. Fira, I. Horbachevsky Ternopil National Medical University

PhD in Biological Sciences, Professor, head of the Department of Pharmacy, I. Horbachevsky

P. G. Lykhatskyі, I. Horbachevsky Ternopil National Medical University

professor of the Department of Medical Biochemistry, I. Horbachevsky

References

Voloshyn, O. I., Pishok, O. V., Voloshyna, L. O. (2003). Fitoterapiia, 3, 3–7.

Braun, L., Cohen, M. (2007). Herbs and natural supplements : An Evidence-based Guide. (2nd Edition). Marrickville NSW : Elsevier, 567.

Hutsol, V. V., Zhuravel, I. O., Hur’ieva, I. H. (2017). Fitoterapiia. Chasopys, 1, 55–58.

Kosev, P. A. (2011). Lekarstvennye rasteniia : samyi polnyi spravochnik. Moscow: Eskmo–Press, 939.

Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv : metod. rek. Kyiv.

Lushchak, V. I., Bahniukova, T. V., Lushchak, O. V. (2004). Ukrainskyi biokhimichnyi zhurnal, 26, 136–141.

Reitman, S., Frankel, S. (1957). Definition of biochemical indicators of the toxicity of liver. Amer. J. Clin. Path., 28 (1), 56–60.

Antoniak, H. L., Vazhnenko, O. V., Panas, N. Ye. (2011). Naukovyi visnyk LNUVMBT imeni S. Z. Hzhytskoho, 3 (2 (48)), 322–332.

Koroliuk, M. A., Ivanova, L. I., Maiorova, I. G. (1988). Lab. delo, 1, 16–19.

Gross, D., & Tolba, R. H. (2015). Ethics in Animal–Based Research. European Surgical Research, 55 (1–2), 43–57. https://doi.org/10.1159/000377721

Rebrova, O. Yu. (2006). Statisticheskiy analiz meditsinskikh dannykh. Primeneniye paketa prikladnykh programm STATISTICA. Moscow : MediaSfera, 312.

Badzhinian, S. A. (2016). Meditcinskaia nauka Armenii NAN RA, LVI (2), 12–20.

Zyn, A. (2012). Visnyk Lvivskoho universytetu. Seriia biolohichna, 60, 21–39.

Finkel, T. (2011). Signal transduction by reactive oxygen species. The Journal of Cell Biology, 194 (1), 7–15. https://doi.org/10.1083/jcb.201102095

Reznikov, O. H. (2014). Visnyk NAN Ukrainy, 10,17–28.

Kuzmak, I. P. (2013). Visnyk problem biolohii i medytsyny, 1 (2 (99)), 121–124.

Downloads

Published

2019-09-02

Issue

No. 3(60) (2019)

Section

Pharmacology and biochemistry

License

Copyright (c) 2019 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).