Carboxilic acids of Nigella Sativa and N. Damascenа seeds
DOI:
https://doi.org/10.24959/ubphj.19.229Keywords:
Nigella arvensis, Nigella damascene, carboxylic acids, chromato-mass-spectrometric analysisAbstract
Topicality. Despite the use of seeds of several species of Nigella L. genus in different countries folk medicine and
the availability of experimental data on their ability to detect a number kinds of biological activity, these plants raw materials are not included to Ukrainian and worlds’ Pharmacopoeias. There are no medicines including components of Nigella seeds in the “State Register of Medicinal Products of Ukraine” (2019). Scientific data on carboxylic acids content in raw material of these plants is limited mainly by the component composition of main fatty acids, so the relevance of such studies is beyond doubt.
Aim. To analyze the component composition and content of carboxylic acids in seeds of two representatives of the Nigella genus – N. damascena and N. arvensis.
Materials and methods. The raw materials were harvested from plants grown on the territory of the Ternopil region. Investigations were conducted by the chromato-mass-spectrometric method. The Agilent Technologies 6890 N
gas chromatograph with 5973 N mass spectrometry detector was used.
Results and discussion. There were detected 15 fatty acids in N. arvensis seeds and 14 in N. damascena seeds. Unsaturated oleic and linoleic fatty acids prevailed in the raw materials of both species; their total content was 85095 mg/kg in the N. arvensis s and 101139 mg/kg in the N. damascena seeds. The presence of levulinic, succinic, malic, 10-oxo-8-decenic and citric acid were established among aliphatic acids of both species. Among the aromatic compounds, benzoic acid was found in the raw materials of both species (132mg/kg in N. arvensis and 1167 mg/kg in N.damascena), whereas phenylacetic acid and 2-amino-3-methoxybenzoic acid were found only in N. damascena seeds (1504 mg/kg
and 232 mg/kg, respectively).
Conclusions. The contents of 22 carboxylic acids in the seeds of N. arvensis and N. damascena were established by chromato-mass-spectrometric method. Due to the high content of unsaturated fatty acids and aromatic acids, N. damascena seeds can be considered as a more promising potential source of anticholosterinemic, antioxidant and antiseptic medicines compared to N. arvensis.
References
The Plant List. (2019). Available at : http://www.theplantlist.org
Dobrochaeva, D. N., Kotov, M. I., Prokudin, Iu. N. (1999). Opredelitel vysshikh rastenii Ukrainy. Kiev : Fіtosotcіotcentr, 548.
Rudenka, D. M., Shanaidab M. I. (2014). Tezy materialiv Vseukrainskoi naukovo–praktychna konferentsiia “Aktualni pytannia eksperymentalnoi i klinichnoi biokhimii ta farmakolohii” (9–10. 10. 2014). Medychna khimiia, 16 (3 (60)), 134.
Kooti, W., Hasanzadeh–Noohi, Z., Sharafi–Ahvazi, N., Asadi–Samani, M., & Ashtary–Larky, D. (2016). Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa ). Chinese Journal of Natural Medicines, 14 (10), 732–745. https://doi.org/10.1016/s1875-5364(16)30088-7
Ahmad, A., Husain, A., Mujeeb, M., Khan, S. A., Najmi, A. K., Siddique, N. A., … Anwar, F. (2013). A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3 (5), 337–352. https://doi.org/10.1016/s2221-1691(13)60075-1
Havlik, J., Kokoska, L., Vasickova, S., & Valterova, I. (2006). Chemical composition of essential oil from the seeds ofNigella arvensis L. and assessment of its actimicrobial activity. Flavour and Fragrance Journal, 21 (4), 713–717. https://doi.org/10.1002/ffj.1713
Sieniawska, E., Sawicki, R., Golus, J., Swatko-Ossor, M., Ginalska, G., & Skalicka-Wozniak, K. (2018). Nigella damascena L. Essential Oil–A Valuable Source of β-Elemene for Antimicrobial Testing. Molecules, 23 (2), 256. https://doi.org/10.3390/molecules23020256
Derzhavnyi reiestr likarskykh zasobiv Ukrainy. (2019). Avaikable at : http://www.drlz.com.ua
Benzel, I. L., Darmohrai, R. Ye., Benzel, L. V. (2010). Farmatsevtychnyi zhurnal, 1, 98–101.
Gontova, T. M. (2013). Farmacevtychnyj chasopys, 1, 44–46. https://doi.org/10.11603/2312-0967.2013.1.2308
Marchyshyn, S. M., Shanajda, M. I., Dub, A. I. (2014). Farmacevtychnyj chasopys, 4 (32), 13–16. https://doi.org/10.11603/2312-0967.2014.4.3442
Rudnyk, A. M. (2014). Farmacevtychnyj chasopys, 4 (32), 34–37. https://doi.org/10.11603/2312-0967.2013.4.2438
Yurchenko, N. S., Il`yina, T. V., Koval`ova, A. M. (2012). Ukrayins`ky`j biofarmacevty`chny`j zhurnal, 5-6, 104–107.
Marchyshyn, S. M., Shanayda, M. I., Kernychna, I. Z., Demydiak, O. L., Dahym, I. S., Berdey, T. S., & Potishnyj, I. M. (2016). Qualitative composition and organi c acids content in the abovegroun d part of plants fro m families lamiaceae, asteraceae, apiaceae and chenopodiaceae. International Journal of Medicine and Medical Research, (1). https://doi.org/10.11603/ijmmr.2413-6077.2015.2.6374
Skakovskiy, E. D., Tychinskaya, L. Yu., Shish, S. N., Shutova, A. G., Lamotkin, S. A., Kryvonosova, E. V. (2015). NMR analysis of chloroform extracts of Nigella seeds. Proceedings of BSTU, Minsk, 4, 164–167.
Shanaida, M., Kernychna, I., Shanaida, Yu. (2017). Chromatographic analysis of organic acids, amino acids, and sugars in Ocimum americanum L. Acta Poloniae Pharmaceutica – Drug Research, 74 (2), 729–732.
Carrapiso, A. I., & García, C. (2000). Development in lipid analysis: Some new extraction techniques and in situ transesterification. Lipids, 35 (11), 1167–1177. https://doi.org/10.1007/s11745-000-0633-8
Mahmmoud, Y. A., & Christensen, S. B. (2011). Oleic and linoleic acids are active principles in Nigella sativa and stabilize an E2P conformation of the Na,K-ATPase. Fatty acids differentially regulate cardiac glycoside interaction with the pump. Biochimica et Biophysica Acta (BBA) – Biomembranes, 1808 (10), 2413–2420. https://doi.org/10.1016/j.bbamem.2011.06.025
Downloads
Published
Issue
Section
License
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
