Impact of succinate derivatives on oxidant/ antioxidant balance system in the rat pancreas and liver
Keywords:succinate derivatives, pancreas, liver, lipid peroxidation, antioxidant enzymes
Topicality. The succinate derivatives are widely used as a basis for the metabolite type drugs development which possess various pharmacological effects. The start of drugs production and their medical use depends directly on their safety for health. The xenobiotic safety assessment involves the identification of such changes that may be associated with mechanisms of adaptation or damage in organs and systems of the organism.
Aim. To determine the nature of succinate derivatives’ impact on the indices of the lipid peroxidation and antioxidant system in pancreas and liver of rats.
Materials and methods. The succinate derivatives were administered to rats orally 30-fold. The biological material studied were the homogenate of pancreas and liver. We determined the content of the dienic conjugates, lipid hydroperoxides and thiobarbituric acid reactive substances. We also verified the activeness of catalase, superoxide dismutase, glutathione peroxidase and glutathione S-transferasees.
Results and discussion. We found the influence of succinate derivatives in sub-toxic doses characterized by increased intensity of the lipid peroxidation in the pancreas interconnected with the changes in the activity of these reactions in the liver. These effects were determined due to the antioxidant enzymes activity decrease. The compounds’ impact differed in the degree
of disorders in the oxidative-antioxidant organs homeostasis . We have registered that the influence of relatively low doses of the succinate derivatives caused less severe and multidirectional changes in the lipid peroxidation indices and signs of the enhanced the antioxidant protection in the studied organs.
Conclusions. The succinate derivatives course changes in the activity of the free radical oxidation and antioxidant
system in pancreas and liver thus can affect the state of the metabolic processes in organism.
Evglevskij, A. A., Ryzhkova, G. F., Evglevskaja, E. P., Vanina, N. V., Mihajlova, I. I., Denisova, A. V., & Eryzhenskaja, N. F. (2013). Vestnik Kurskoj gosudarstvennoj sel’skohozjajstvennoj akademii, 3, 67–69.
Simonian, E. V., Shikova, Iu. V. (2014). Uspekhi sovremennogo estestvoznanii, 12 (3), 231–235.
Iasnetcov, V. V., Prosvirova, E. P., Tcublova, E. G. (2012). Eksperimentalnaia i klinicheskkaia farmakologiia, 75 (7), 8–10.
Tretter, L., Patocs, A., & Chinopoulos, C. (2016). Succinate, an intermediate in metabolism, signal transduction, ROS, hypoxia, and tumorigenesis. Biochimica et Biophysica Acta (BBA) – Bioenergetics, 1857 (8), 1086–1101. https://doi.org/10.1016/j.bbabio.2016.03.012.
Horbenko, N. I. (2004). Patohenetychne obgruntuvannia efektyvnosti pokhidnoho yantarnoi kysloty – fensuktsynalu v terapii tsukrovoho diabetu
ta yoho sudynnykh uskladnen (eksperymentalne doslidzhennia). Extended abstract of Doctor’s thesis. Kharkiv, 36.
Guengerich, F. P. (2011). Mechanisms of Drug Toxicity and Relevance to Pharmaceutical Development. Drug Metabolism and Pharmacokinetics, 26 (1), 3–14. https://doi.org/10.2133/dmpk.dmpk-10-rv-062
Lenzen, S. (2017). Chemistry and biology of reactive species with special reference to the antioxidative defence status in pancreatic β-cells. Biochimica et Biophysica Acta (BBA) – General Subjects, 1861 (8), 1929–1942. https://doi.org/10.1016/j.bbagen.2017.05.013
Platser, Z., Vydlakova, M., Kupyla, L. (1970). Chekhosl. med. obzor, 16 (1), 30–34.
Asakawa, T., Matsushite, S. (1980). Colorings conditions of thiobarbituric acid test for detecting lipid hydroperoxides. Lipids, 15 (3), 137–140. https://doi.org/10.1007/ BF02540959
Stalnaia, I. D., Garishvili, T. G. (1977). Sovremennye metody v biokhimii. Moscow, 66–68.
Koroliuk, M. A., Ivanova, L. I., Maiorova, I. G., Tokarev, V. E. (1988). Laboratornoe delo, 1, 16–19.
Arutiunian, A. V., Dubinina, E. E., Zybina, N. N. (2000). Metody otcenki svobodno-radikalnogo okisleniia i antioksidantnoi sistemy organizma: metod. rekomendatcii. St Petersburg: Foliant, 104.
Ovsiannіkova, L. M., Alokhіna, S. M., Drobіnska, O. V. (1999). Bіokhіmіchnі ta bіofіzichnі metodi otcіnki porushen okisliuvalnogo gomeostazu v osіb, shcho zaznali radіatcіinogo vplivu vnaslіdok avarії na ChAES : metod. rekomendatcії. Kyiv, 18.
Gasparov, V. S., Degtiar, V. G. (1994). Biokhimiia, 59 (6), 763–775.
Kandola, K., Bowman, A., & Birch–Machin, M. A. (2015). Oxidative stress – a key emerging impact factor in health, ageing, lifestyle and aesthetics. International Journal of Cosmetic Science, 37, 1–8. https://doi.org/10.1111/ics.12287
Chekman, I. S., (Ed.). (2013). Farmakolohiia: pidruchnyk dlia studentiv vyshchykh navchalnykh zakladiv. Vinnyitsya : Nova knyiga, 792.
Kudrya, M. Ya., Lalyimenko, O. S., Zavgorodniy, I. V., Melnikovskaya N. Vic., Ustenko, N. V., Shalamay, A. S. (2016). Ukrayinskiy Zhurnal z Problem Meditsini Pratsi, 4 (49), 73–81.
Czaja, A. J. (2016). Nature and Implications of Oxidative and Nitrosative Stresses in Autoimmune Hepatitis. Digestive Diseases and Sciences, 61 (10),
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