DOI: https://doi.org/10.24959/ubphj.19.232

Study of the Reishi mushrooms dry extract hepatoprotective properties

I. Herasymets, L. Fira, I. Medvid

Abstract


Topicality. It is promising to search for new effective hepatoprotectors of natural origin, which are aimed at normalizing homeostasis in liver, increasing resistance to pathogenic influences, normalizing functional activity of liver, stimulating regenerative processes. Therefore, the issues of providing these drugs with a therapeutic process are relevant.
Aim. To investigate the hepatoprotective properties of the Reishi mushrooms dry extract in an experiment on rats with simulated paracetamol hepatitis.
Materials and methods. Studies were conducted on 60 white male rats, weighing 180-210 g at the age of 5-5.5 months. The rats were divided into 10 groups, each of which included 6 animals. Acute hepatitis was modeled by intragastric paracetamol administering at a dose of 1250 mg/kg 1 time per day as a suspension in a 2 % starch gel solution for 2 days. Correction of toxic damage was performed with dry extract of Reishi mushrooms, which was administered intragastric 2 hours before the administration of paracetamol and daily after the lesion at a dose of 100 mg/kg of body weight. “Silibor” was selected as a comparison drug, which was administered according to the same scheme as Reishi extract at a dose of 20 mg/kg of animal body weight. Euthanasia of rats was performed using barbamyl sodium on the 3rd, 7th and 10th day from the onset of the lesion.
Results and discussion. The study subjected the homogenate of liver and serum. Blood was taken from the heart of animals. Endogenous intoxication of animals was assessed by the AlAT, AsAT, GGTP, ALP activity and the value of thymol sample after the corrective factors introduction.
Conclusions. Studies have found a positive effect of Reishi mushrooms dry extract on the permeability of plasma membranes of hepatocytes in animals with paracetamol hepatitis, which is confirmed by the normalization of the activity of membrane-dependent enzymes – AlAT, AsAT, GGTP, ALP and a decreasing of thymol sample. This indicates the hepatoand membrane-protective properties of the investigated phytopreparation and will allow its further successful use in liver diseases.


Keywords


Reishi mushrooms; paracetamol; hepatitis; dry extract; hepatoprotective properties

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Abbreviated key title: Ukr. biopharm. j.

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