DOI: https://doi.org/10.24959/ubphj.19.236

Research of the phenolcarboxylic acids of Helichrysum bracteatum

A. M. Moskalenko, N. V. Popova

Abstract


Topicality. The pharmaceutical industry requires an increase in the range and quantity of medicinal plants to produce new effective drugs. Drugs made on the basis of medicinal plants have a diverse and multifactorial pharmacological action. Expanding the range of medicinal plants, primarily due to the study of medicinal plants, widely used in traditional medicine, and plants systematically close to officinal plants. Phenolcarbonic acids have a high pharmacological effect and can be important as independent biologically active substances for medicines. In this regard, a special interest is the search for plants that contain a high content of phenol carboxylic acids, while having an extensive raw material base.
Aim. To study phenol carboxylic acids in herbal drugs of immortelle (Helicrysum bracteatum) by paper-, TLC and
HPLC.
Materials and methods. For the study, the herbal drugs of the immortelle was used. The analysis was carried out
separately in the flowers and herb of the plant. To identify phenolcarboxylic acids in the objects under study, 50 % aqueousalcoholic extracts were used, which were subjected to chromatographic analysis using thin-layer chromatography. For HPLC analysis, chromatography was performed on an Agilent Technologies 1200 liquid chromatograph. Methanol and a 0.1 % solution of formic acid in water were used as the mobile phase.
Results and discussion. As a result of the analysis, 8 phenolcarboxylic acids were determined in the herbal drugs under investigation: gallic, hydroxyphenyl acetic, caffeic, coumarovic, ferulic, synapic, cinamic, quinic acids. At the same time, quinic acid (33.11 %), synapic acid (29.91 %) and coumaric acid (20.55 %) are in large quantities in flowers, and quinic acid (85.23 %) is the main herb among phenol carboxylic acids. The remaining phenol carboxylic acids are contained in much smaller quantities.
Conclusions. For the first time, a study was conducted on the qualitative composition and content of herb phenol
carboxylic acids and flowers of immortelle bracts using TLC and HPLC methods. The data obtained from this study indicate the promise of further phytochemical studies of the immortelle of the bracts and the development on its basis of new drugs and dietary supplements.


Keywords


immortelle; phenol carbonic acid; thin layer chromatography; HPLC

References


Shkarina E. I., Maksimova T. V., Nikulina I. N. (2001). Him.–farmac. zhurn., 35 (6), 40–47.

Gardner, C. A. (1990). Wildflowers of Western Australia (17th ed.). Perth, Western Australia : St. GeorgBooks, 144.

Moskalenko, A. N., Popova, N. V., Litvinenko, V. I. (2018). Fenol’nye soedinenija : svojstva, aktivnost’, innovacii: sbornik nauchnyh statej po materialam X Mezhdunarodnogo simpoziuma “Fenol’nye soedinenija : fundamental’nye i prikladnye aspekty”. Moskva : IFR RAN, 333–339.

Moskalenko, A., & Popova, N. (2018). Research of mineral composition of Helichrysum bracteatum herbal drugs. Ukraïns’kij bìofarmacevtičnij žurnal, 1 (54), 72–76. https://doi.org/10.24959/ubphj.18.160

Moskalenko, A. N., Popova, N. V., Gladuh, E. V. (2018). East European Scientific Journal, 5 (33), 49–55.

Moskalenko, A., & Popova, N. (2018). Study of immortelle (Helichrysum bracteatum) fatty acids. Ukraïns’kij bìofarmacevtičnij žurnal, 4 (57), 64–68.

https://doi.org/10.24959/ubphj.18.187

World Health Organization Geneva. (2003). WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants, 72.

Harborne J. B., and Mabry T. J. (1982) The flavonoids : Advances in Research. Chapman and Hall, London. 744p.

Seeram, N. P., Lee, R., Scheuller, H. S., & Heber, D. (2006). Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food Chemistry, 97 (1), 1–11. https://doi.org/10.1016/j.foodchem.2005.02.047

Komanceva, E. V., Frolova, O. O., Dement’eva, T. M. (2014). Izuchenie soderzhanija fenolokislot v nekotoryh vidah ivy. Innovacionnye tehnologii v farmacii: sb. nauch.–metod. tr. Irkutsk : IGMU, 114–115.

Kuhta, V. K., Oleckij Z. I., Taganovich A. D. (2008). Biologicheskaja himija: uchebnik. Minsk : Izdatel’stvo BINOM, 688.


GOST Style Citations


1. О влиянии биологически активных веществ на антиоксидантную активность фитопрепаратов / Е. И. Шкарина и др. // Хим.–фармац. журн. – 2001. – Т. 35, № 6 – С. 40–47.


2. Gardner, C. A. Wildflowers of Western Australia (17th ed.) / C. A. Gardner. – Perth, Western Australia : St. GeorgBooks, 1990. – 144 p.


3. Москаленко, А. Н. Исследования фенольных соединений травы бессмертника прицветникового / А. Н. Москаленко, Н. В. Попова, В. И. Литвиненко // Фенольные соединения: свойства, активность, инновации : сб. науч. статей по матер. X Междунар. симпозиума «Фенольные соединения : фундаментальные и прикладные аспекты», Москва, 14–19 мая 2018 г. – М. : ИФР РАН, 2018 – C. 333–339.


4. Москаленко, А. M. Дослідження мінерального складу сировини безсмертника приквіткового (Helichrysum bracteatum) / А. M. Москаленко, Н. В. Попова // Укр. біофармац. журн. – 2018. – № 1 (54). – C. 72–76. https://doi.org/10.24959/ubphj.18.160


5. Москаленко, А. Н. Изучение аминокислотного состава сырья бессмертника прицветникового (Helichrysum braceatum) / А. Н. Москаленко, Н. В. Попова, Е. В. Гладух // East Eur. Sci. J. – 2018 – Vol. 5 (33). – P. 49–55.


6. Москаленко, А. M. Дослідження складу жирних кислот безсмертника приквіткового (Helichrysum bracteatum) / А. Н. Москаленко, Н. В. Попова // Укр. біофармац. журн. – 2018. – № 4 (57). – C. 64–68. https://doi.org/10.24959/ubphj.18.187


7. WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants / World Health Organization Geneva. – 2003. – 72 р.


8. Harborne, J. B. The flavonoids: Advances in Research / J. B. Harborne, T. J. Mabry. – Chapman and Hall, London, 1982. – 744 p.


9. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy / N. P. Seeram, R. Lee, H. S. Scheuller, et al. // Food Chemistry. – 2006. – Т. 97, № 1. – С. 1–11. https://doi.org/10.1016/j.foodchem.2005.02.047


10. Команцева, Е. В. Изучение содержания фенолокислот в некоторых видах ивы / Инновационные технологии в фармации : сб. науч.–метод. тр. // Е. В. Команцева, О. О. Фролова, Т. М. Дементьева ; под общей ред. Е. Г. Горячкиной. – Иркутск : ИГМУ, 2014. – С.114–115.


11. Биологическая химия : учебник / В. К. Кухта, З. И. Олецкий., А. Д. Таганович ; под ред. А. Д. Тагановичрафиа. – Мн : Асар, М. : Издательство
БИНОМ, 2008. — 688 с.





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