DOI: https://doi.org/10.24959/ubphj.19.238

Extraction and purification of curcuminoids from Curcuma longa L. rhizome

D. Pylypenko, Yu. Krasnopolsky

Abstract


Topicality. Curcumin is a highly effective natural lipophilic antioxidant and a rich body of evidence confirming its safety and efficacy has been accumulated. Currently in Ukraine there is no commercial high purified pharmaceutical substance of this bioflavonoid.
Aim. To extract and purify curcuminoids from Curcuma Longa L. rhizome. Materials and methods. Chromatography methods were used to assess the quality of curcuminoids: thin-layer and high-performance liquid chromatography. The quantitative determination of the curcuminoids was carried out spectrophotometrically at 540 nm.
Results and discussion. Curcumin solubility in organic solvents with different polarity was compared (hexane, acetone, chloroform, ethanol, methanol). The optimal conditions of curcuminoids extraction were determined, such as the type of extractant, the ratio of raw material to extractant, duration, temperature, etc. The conditions of curcuminoids precipitation were investigated in order to purify the extract from the contaminants. Column chromatography on silica gel was used to obtain highly purified curcumin.
Conclusions. The scheme of obtaining a highly purified curcuminoids solution with a content of curcuminoids at least 90 %, and among them 72 % of diferuloylmethane, was proposed.


Keywords


antioxidants; bioflavonoids; curcumin; extraction; chromatography

References


Menshikova, E. B., Lankin, V. Z., Kandalintseva, N. V. (Eds.). (2012). Fenolnyie antioksidantyi v biologii i meditsine. Saarbrücken : LAP LAMBERT Academic Publishing, 495.

Alisi, I. O., Uzairu, A., Abechi, S. E., & Idris, S. O. (2018). Evaluation of the antioxidant properties of curcumin derivatives by genetic function algorithm. Journal of Advanced Research, 12, 47–54. https://doi.org/10.1016/j.jare.2018.03.003

Alrawaiq, N. S., Abdullah, A. (2014). A review of antioxidant polyphenol curcumin and its role in detoxification. International Journal of PharmTech Research, 6 (1), 280–289.

Katsai, O. G., Ruban, O. A., Krasnopolskyi, Y. M. (2018). “Quality-by-Design” approach to the development of a dosage form the liposomal delivery system of cytochrome C. Pharmakeftiki, 30 (2), 76–87.

Pylypenko, D. M., Gorbach, T. V., Katsai, O. G., Grigoryeva, A. S., Krasnopolsky, Yu. M. (2019). A study of oxidative stress markers when using the liposomal antioxidant complex. Pharmakeftiki, 31 (1), 40–47.

Togni, Di Pierro, F., Rapacioli, G., Di Maio, E. A., Appendino, G., & Franceschi, F. (2013). Comparative evaluation of the pain-relieving properties of a lecithinized formulation of curcumin (Meriva®), nimesulide, and acetaminophen. Journal of Pain Research, 201–205. https://doi.org/10.2147/JPR.S42184

Huang, M., Su, E., Zheng, F., & Tan, C. (2017). Encapsulation of flavonoids in liposomal delivery systems: the case of quercetin, kaempferol and luteolin. Food & Function, 8 (9), 3198–3208. https://doi:10.1039/c7fo00508c

Gang, W., Jie, W. J., Ping, Z. L., Ming, D. S., Ying, L. J., Lei, W., & Fang, Y. (2012). Liposomal quercetin: evaluating drug deliveryin vitroand biodistributionin vivo. Expert Opinion on Drug Delivery, 9 (6), 599–613. https://doi.org/10.1517/17425247.2012.679926

Pescosolido, N., Giannotti, R., Plateroti, A., Pascarella, A., & Nebbioso, M. (2013). Curcumin: Therapeutical Potential in Ophthalmology. Planta Medica, 80 (04), 249–254. https://doi.org/10.1055/s-0033-1351074

Shvetc, V. I., Krasnopolskii, Iu. M., Sorokoumova, G. M. (2016). Liposomalnye formy lekarstvennykh preparatov : tekhnologicheskie osobennosti polucheniia i primenenie v klinike. Moscow : Remedium, 200.

Derzhavna Farmakopeya Ukraїni: (Vols. 1–3). (2015). Derzhavne pіdprimstvo “Ukraїns’kij naukovij farmakopejnij centr yakostі lіkars’kih zasobіv”. (2-edition). Kharkiv : Derzhavne pіdprimstvo “Ukraїns’kij naukovij farmakopejnij centr yakostі lіkars’kih zasobіv”, 1, 1128.

Kapustin, M. A., Chubareva, A. S., Tcyganov, V. G., Kurchenko, V. P. (2016). Trudy Belorusskogo GU, 11 (2), 248–262.

Kurkin, V. A., Kataev, V. A., Latyipova, G. M., Farhutdinov, R. R., Avdeeva, E. V., Borisov, M. Yu., Ryazanova, T. K., Serebryakova, A. D., Dudareva, L. V. (2016). Patent RU No 2650642C1. Antioksidantnoe sredstvo “kurkumin ekstrakt gustoi”.


GOST Style Citations


1. Меньшикова, Е. Б. Фенольные антиоксиданты в биологии и медицине / Е. Б. Меньшикова, В. З. Ланкин, Н. В. Кандалинцева. – Saarbrücken : LAP LAMBERT Academic Publishing, 2012. – 496 р.


2. Evaluation of the antioxidant properties of curcumin derivates by genetics function algorithm / I. O. Alisi, A. Uzairu, S. E. Abechi, S. O. Idris // J. of Advanced Res. – 2018. – Vol. 12. – P. 47–54. https://doi.org/10.1016/j.jare.2018.03.003


3. Alrawaiq, N. S. A review of antioxidant polyphenol curcumin and its role in detoxification / N. S. Alrawaiq, A. Abdullah // Intern. J. of Pharm. Tech. Res. – 2014. – Vol. 6, № 1. – P. 280–289.


4. Katsai, O. “Quality-by-Design” approach to the development of a dosage form the liposomal delivery system of cytochrome C / O. Katsai, O. Ruban, Y. Krasnopolskyi // Pharmakeftiki. – 2018. – Vol. 30, № 2. – P. 76–87.


5. A study of oxidative stress markers when using the liposomal antioxidant complex / D. M. Pylypenko, T. V. Gorbach, O. G. Katsai et al. // Pharmakeftiki. – 2019. – Vol. 31, № 1. – P. 40–47.


6. Comparative evaluation of the pain releving properties of lecithinized formulation of curcumin (Meriva), nimesulide and acetaminofen / F. Di Pierro, G. Rapacioli, E. A Di Maio et al. // J. of Pain Res.– 2013. – Vol. 6. – P. 201–205. https://doi.org/10.2147/JPR.S42184


7. Encapsulation of flavonoids in liposomal delivery systems : the case of quercetin, kaempferol and luteolin / M. Huang, E. Su, F. Zheng, C. Tan // Food Function. – 2017. – Vol. 8, № 9. – P. 3198–3208. https://doi:10.1039/c7fo00508c


8. Liposomal quercetin : evaluating drug delivery in vitro and biodistribution in vivo / W. Gang, W. J. Jie, Z. L. Ping et al. // Expert Opinion on Drug Delivery. – 2012. – Vol. 9, № 6. – P. 599–613. https://doi.org/10.1517/17425247.2012.679926


9. Curcumin : Therapeutical Potential in Ophthalmology / N. Pescosolido, R. Giannotti, A. M. Platerodi et al. // Planta Med. – 2014. – Vol. 80, № 4. – P. 249–254. https://doi.org/10.1055/s-0033-1351074


10. Швец, В. И. Липосомальные формы лекарственных препаратов: технологические особенности получения и применение в клинике / В. И. Швец, Ю. М. Краснопольский, Г. М. Сорокоумова. – М. : Ремедиум, 2016. – 200 c.

 
11. Державна фармакопея України : в 3-х т. / Державне підприємство «Український науковий фармакопейний центр якості лікарських засобів». – 2–е вид. – Х. : Державне підприємство «Український науковий фармакопейний центр якості лікарських засобів», 2015. – Т. 1. – 1128 с.

 

12. Выделение куркуминоидов из кореневища Curcuma Longa L. и исследование состава полученного препарата с использованием хроматографического метода анализа / М. А. Капустин, А. С. Чубарева, В. Г. Цыганов, В. П. Курченко // Труды Белорусского ГУ. – 2016. – Т. 11, № 2. – С. 248–262.


13. Пат. 2650642C1. Российская Федерация, МПК A 61 K 36/9066, B 01 D 11/02, A 61 P 39/06. Антиоксидантное средство «куркумин экстракт густой» / Куркин В. А., Катаев В. С., Латыкова Г. М. и др.; заявитель и патентообладатель ФГБОУ ВО СамГМУ Минздрава России. – № 2016145500; заявл. : 21.11.16 ; опубл. : 16.04.18. – Бюл. № 11. – 10 c.





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)