Extraction and purification of curcuminoids from Curcuma longa L. rhizome
DOI:
https://doi.org/10.24959/ubphj.19.238Keywords:
antioxidants, bioflavonoids, curcumin, extraction, chromatographyAbstract
Topicality. Curcumin is a highly effective natural lipophilic antioxidant and a rich body of evidence confirming its safety and efficacy has been accumulated. Currently in Ukraine there is no commercial high purified pharmaceutical substance of this bioflavonoid.
Aim. To extract and purify curcuminoids from Curcuma Longa L. rhizome. Materials and methods. Chromatography methods were used to assess the quality of curcuminoids: thin-layer and high-performance liquid chromatography. The quantitative determination of the curcuminoids was carried out spectrophotometrically at 540 nm.
Results and discussion. Curcumin solubility in organic solvents with different polarity was compared (hexane, acetone, chloroform, ethanol, methanol). The optimal conditions of curcuminoids extraction were determined, such as the type of extractant, the ratio of raw material to extractant, duration, temperature, etc. The conditions of curcuminoids precipitation were investigated in order to purify the extract from the contaminants. Column chromatography on silica gel was used to obtain highly purified curcumin.
Conclusions. The scheme of obtaining a highly purified curcuminoids solution with a content of curcuminoids at least 90 %, and among them 72 % of diferuloylmethane, was proposed.
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