The effect of atristamine on prooxidant-antioxidant balance in the rat brain after traumatic brain injury
Keywords:atristamine, 2-methyl-3-(phenylaminomethyl)-1H-quinolin-4-one, traumatic brain injury, cerebroprotective activity, lipid peroxidation, antioxidant defense systems
Topicality. One of the possible mechanisms for the realization of the cerebroprotective properties of atristamine proven in previous studies is the effect on prooxidant-antioxidant balance in the brain tissue of animals, which greatly predetermines the further development of traumatic brain injury consequences.
Aim. To ascertain the nature of atristamine effect on prooxidant-antioxidant balance in the rat brain after traumatic brain injury.
Materials and methods. Indicators of lipid peroxidation and antioxidant defense were determined in rat brain homogenate on the 3rd day after traumatic brain injury.
Results and discussion. According to the obtained results, the significant reduction of superoxide dismutase activity (by 50.8 %), the decrease in contents of TBA-reactants (by 3.1 times) and oxidative modification products of proteins, the increase in of diene conjugates content (by 2.4 times) were observed after treatment using atristamine in comparison with the indicators of the disease group. It should be noted that significant decrease in the content of reduced glutathione was observed (by 2.1 times) in the group of animals treated with atristamine compared to the intact control group. Furthermore, this indicator in the atristamine group tended to decrease (by 41.6%) compared to that in the disease group.
Conclusions. The analysis of experimental data showed that atristamine probably does not possess a direct antioxidant effect. Evidently, it actively involves the glutathione system to the antioxidant defense of the brain and switches the implementation of antioxidant mechanisms to the utilization of reduced glutathione with a dominant effect on preventing the formation of secondary products of lipid peroxidation at the expense of active use of the glutathione system resources.
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