Investigation of combined gel antimicrobial activity for the treatment of oral cavity infectious-inflammatory diseases
DOI:
https://doi.org/10.24959/ubphj.19.245Keywords:
gel, antimicrobial activity, metronidazole benzoate, miramistin, compositionAbstract
Topicality. Treatment of periodontal inflammatory disease is one of the most pressing problems of dentistry. According to the modern point of view, inflammatory periodontal diseases are infectious chronic inflammatory diseases, that is why to normalize oral microflora is a continuous condition of their rational therapy. In chronic gingivitis and periodontitis the number of anaerobic bacteria strains increases to 70-80 %, whereas in normal their number does not exceed 20-30 %. This explains the feasibility of topical use of anaerobic drugs in the treatment of inflammatory periodontal diseases.
Aim. To substantiate and to study the antimicrobial activity of the combined gel intended for the treatment of oral cavity infectious-inflammatory diseases.
Materials and methods. We used biological pharmacopoeial method of research (study of antimicrobial preservatives effectiveness).
Results and discussion. According to the results of studies of antimicrobial action in the bacterial inoculation of the tested samples of the gel with serial number 10219 (metronidazole benzoate – 1.60 g; miramistin – 0.50 g; hyaluronic acid – 0.20 g) and serial number 40219 (metronidazole benzoate – 1.60 g; miramistin – 0.50 g). At specified intervals, viable aerobic microorganisms S. aureus, P. aeruginosa and the yeast and mold fungi C. albicans, A. brasiliensis were not detected, and the logarithm for their decrease was more than 5. However, in the studied series 20219 (metronidazole benzoate – 1.00 g; miramistin – 0.50 g), at 14 and 28 days after inoculation from the test samples for appropriate nutrient media, and in series 30219 (metronidazole benzoate – 1.20 g; miramistin – 0.20 g) for 14 days, there was a significant increase in the colonies of all microorganisms compared to their number at the previous control point. There was no logarithm for reducing the number of viable aerobic microorganisms and yeast and mold fungi. Therefore, studies have shown that the developed gel with serial numbers 10219 and 40219 withstand the antimicrobial efficacy test in accordance with the requirements of SPhU *, section 5.1.3, for oromucosal drugs. Therefore, for further studies we will use the specified model samples.
Conclusions. We theoretically and experimentally substantiated the development of the combined gel, proved the concentration of active pharmaceutical ingredients with regard to its antimicrobial action.
References
Shmanko, V. V., Kotyk, M. I., & Mykytiv, M. V. (2015). Visnyk Naukovykh Doslidzhen, 4, 71–74. https://doi.org/10.11603/2415-8798.2015.4.5649
Schwach-Abdellaoui, K. Vivien-Castioni, N. Gurny, R. (2000). Local delivery of antimicrobial agents for the treatment of periodontal diseases. European Journal of Pharmaceutics and Biopharmaceutics, 50 (1), 83–99. https://doi.org/10.1016/S0939-6411(00)00086-2
Derzhavna Farmakopeya Ukraїni: (Vols. 1-3). (2015). Derzhavne pіdprimstvo “Ukraїns’kij naukovij farmakopejnij centr yakostі lіkars’kih zasobіv”. (2-edition). Kharkiv: Derzhavne pіdprimstvo “Ukraїns’kij naukovij farmakopejnij centr yakostі lіkars’kih zasobіv”, 1, 1128.
Pertsev, I. M., Dmytriievskyi, D. I., Rybachuk, V. D., Khomenko, V. M., Hudzenko, O. P., Kotenko, O. M., Maslii, Yu. S. (Eds.). (2010). Dopomizhni rechovyny v tekhnolohii likiv: vplyv na tekhnolohichni, spozhyvchi, ekonomichni kharakterystyky i terapevtychnu efektyvnist. Kharkiv: Zoloti storinky, 600.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).