Study of flavonoids dynamic accumulation in the Siberian pea tree shoots
Keywords:Siberian pea tree, Caragana arborescens Lam., legume, flavonoids, quantitative content, spectrophotometry
Topicality. The importance of search and drugs development for the treatment of the hepatobiliary system diseases is determined by their significant proportion among the digestive system diseases. The Siberian pea tree shoots possessing hepatoprotective, antioxidant, and anti-inflammatory effects in the experiment can be used for such preparations development. Flavonoids represent one of the main groups of biologically active compounds of the plant. Therefore, the total flavonoid content can be used as a quality criterion for this raw material standardization.
Aim. To determine the dynamic accumulation of flavonoids in the Siberian pea tree shoots (Cormus Caraganae arborescentis).
Materials and methods. The object of study is the Siberian pea tree shoots harvested during different periods of the plant vegetation from April to August 2018 and 2019 in Kharkiv and Nikolayev regions. A differential spectrophotometry method based on complex formation with aluminum chloride has been used to determine the content of flavonoids in the raw material. Rutin has been used as a standard sample because it prevails among other flavonoids of the Siberian pea tree. The spectra has been recorded using a “Specord 200” spectrophotometer.
Results and discussion. The dynamic accumulation of flavonoids during the vegetation stages of the Siberian pea tree shoots has been studied. The period of mass flowering of the plant was determined as the optimal harvest time.
Conclusions. A methodology for determining the total flavonoid content in the Siberian pea tree shoots has been developed, the optimal harvest term has been established. The results are planned to be used for the standardization of raw materials and herbal preparations of the Siberian pea tree.
Rajaratnam, M., Prystupa, A., Lachowska-Kotowska, P., Załuska, W., & Filip, R. (2015). Herbal medicine for treatment and prevention of liver diseases. Journal of Pre-Clinical and Clinical Research, 8 (2), 55–60. https://doi.org/10.26444/jpccr/71468
Boinik, V. V. (2016). Karagana. Farmatsevtychna entsyklopediia. Kyiv: Morion, 771.
Barnaulov, O. D. & Belodubrovskaja, G. A. (2018). Obzory po klinicheskoj farmakologii i lekarstvennoj terapii, 16(3), 60–67.
Vihtinskaja, I. L., Stepanova, S. I., Vihtinskaja, O. A., Bojnik, V. V. (1997). Lekarstva – Cheloveku: Mezhdunarodnyj sbornik nauchnyh publikacij po sozdaniju i aprobacii novyh lekarstvennyh sredstv, 3, 17–19. Kharkiv.
Zveroboja trava. (2018). Gosudarstvennaja Farmakopeja Rossijskoj Federacii, (XIV-edition), IV. Moscow: FJeMB, 6074–6083.
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).