DOI: https://doi.org/10.24959/ubphj.20.250

Study of flavonoids dynamic accumulation in the Siberian pea tree shoots

S. Stepanova, V. Boynik, T. Gontovaya, O. Filatova

Abstract


Topicality. The importance of search and drugs development for the treatment of the hepatobiliary system diseases is determined by their significant proportion among the digestive system diseases. The Siberian pea tree shoots possessing hepatoprotective, antioxidant, and anti-inflammatory effects in the experiment can be used for such preparations development. Flavonoids represent one of the main groups of biologically active compounds of the plant. Therefore, the total flavonoid content can be used as a quality criterion for this raw material standardization.
Aim. To determine the dynamic accumulation of flavonoids in the Siberian pea tree shoots (Cormus Caraganae arborescentis).
Materials and methods. The object of study is the Siberian pea tree shoots harvested during different periods of the plant vegetation from April to August 2018 and 2019 in Kharkiv and Nikolayev regions. A differential spectrophotometry method based on complex formation with aluminum chloride has been used to determine the content of flavonoids in the raw material. Rutin has been used as a standard sample because it prevails among other flavonoids of the Siberian pea tree. The spectra has been recorded using a “Specord 200” spectrophotometer.
Results and discussion. The dynamic accumulation of flavonoids during the vegetation stages of the Siberian pea tree shoots has been studied. The period of mass flowering of the plant was determined as the optimal harvest time.
Conclusions. A methodology for determining the total flavonoid content in the Siberian pea tree shoots has been developed, the optimal harvest term has been established. The results are planned to be used for the standardization of raw materials and herbal preparations of the Siberian pea tree.


Keywords


Siberian pea tree; Caragana arborescens Lam.; legume; flavonoids; quantitative content; spectrophotometry

References


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GOST Style Citations


1. Herbal medicine for treatment and prevention of liver diseases / M. Rajaratnam, A. Prystupa, P. Lachowska-Kotowska et al. // J. Pre-Clin. and Clin. Res. – 2014. – Vol. 8 (2). – P. 55–60. https://doi.org/10.26444/jpccr/71468

 

2. Бойнік, В. В. Карагана / Фармацевтична енциклопедія // Голова ред. ради та автор предмови В. П. Черних. – 3-тє вид., перероб. і доп. – K. : Моріон, 2016. – 771 c.

 

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4. Фармакологическая активность карфена, выделенного из караганы древовидной / И. Л. Вихтинская, С. И. Степанова, О. А. Вихтинская, В. В. Бойник // Лекарства – Человеку : международ. сб. научн. публ. по созданию и апробации новых лек. средств. – Х., 1997. – Т. 3. – С. 17–19.

 

5. Зверобоя трава // Государственная фармакопея Российской Федерации. – XIV изд. – Т. IV. – М. : ФЭМБ, 2018. – С. 6074–6083.





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Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)