Study of the molecular mass fractions distribution of porcine placenta aqueous extract by the method of highly efficient gel permeation chromatography

Authors

DOI:

https://doi.org/10.24959/ubphj.20.252

Keywords:

pork placenta aqueous extract, high performance gel permeation chromatography method, cosmetic products

Abstract

Topicality. Placenta-based cosmetics are widely used all over the world.
Aim. To investigate the composition of water-soluble porcine placenta fractions and to study the similarity of their components to model membranes.
Materials and methods. The molecular weight distribution of the VESP fractions was studied by high-performance gel permeation chromatography. Molecular weights were determined by the retention time on the column of reference substances. To study the similarity of VESP peptides for model liposome membranes with phosphatidylcholine, the method of fluorescent probes was used.
Results and discussion. VESP contains a sufficient number (24.2 %) of short peptides and free amino acids, a large number of low molecular weight peptides of one or another molecular weight. VESP peptides effectively bind to phosphatidylcholine liposomes.
Conclusions. Due to its rich chemical composition, VESP is a promising raw material for the creation of cosmetic products.

Author Biographies

L. Ivanov, Chuiko Institute of Surface Chemistry National Academy of Sciences of Ukraine

PhD in Chemistry, Leading Researcher

O. Shcherbak, Kharkiv State Zoo Veterinary Academy

PhD in Agriculture, Dean of the Faculty of Biotechnology and Environmental Management, Associate Professor of the Department of Biotechnology

L. Derymedvid, National University of Pharmacy

MD, Professor of the Department of Pharmacology

V. Kravchenko, Ukrainian Medical Stomatological Academy

MD, Professor of the Department of Dermatovenereology

O. Bezugla, State Scientific Institution "Institute for Single Crystals of NAS of Ukraine"

PhD in Pharmacy, Senior Researcher, Head of the Laboratory of Technology and Analysis of Medicines

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Published

2020-03-02

Issue

No. 1(62) (2020)

Section

Biopharmaceutical research

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