Study of the molecular mass fractions distribution of porcine placenta aqueous extract by the method of highly efficient gel permeation chromatography
Keywords:pork placenta aqueous extract, high performance gel permeation chromatography method, cosmetic products
Topicality. Placenta-based cosmetics are widely used all over the world.
Aim. To investigate the composition of water-soluble porcine placenta fractions and to study the similarity of their components to model membranes.
Materials and methods. The molecular weight distribution of the VESP fractions was studied by high-performance gel permeation chromatography. Molecular weights were determined by the retention time on the column of reference substances. To study the similarity of VESP peptides for model liposome membranes with phosphatidylcholine, the method of fluorescent probes was used.
Results and discussion. VESP contains a sufficient number (24.2 %) of short peptides and free amino acids, a large number of low molecular weight peptides of one or another molecular weight. VESP peptides effectively bind to phosphatidylcholine liposomes.
Conclusions. Due to its rich chemical composition, VESP is a promising raw material for the creation of cosmetic products.
Familari, M., Cronqvist, T., Masoumi, Z., & Hansson, S. R. (2017). Placenta-derived extracellular vesicles: their cargo and possible functions. Reproduction, Fertility and Development, 29 (3), 433. https://doi.org/10.1071/rd15143
Burkova, E. E., Grigor’eva, A. E., Bulgakov, D. V., Dmitrenok, P. S., Vlassov, V. V., Ryabchikova, E. I., … Nevinsky, G. A. (2019). Extra Purified Exosomes from Human Placenta Contain an Unpredictable Small Number of Different Major Proteins. International Journal of Molecular Sciences, 20 (10), 2434. https://doi.org/10.3390/ijms20102434
Burkova, E. E., Dmitrenok, P. S., Bulgakov, D. V., Ermakov, E. A., Buneva, V. N., Soboleva, S. E., & Nevinsky, G. A. (2018). Identification of Major Proteins of a Very Stable High Molecular Mass Multi-Protein Complex of Human Placental Tissue Possessing Nine Different Catalytic Activities. Biochemistry & Analytical Biochemistry, 07 (01), 82–94. https://doi.org/10.4172/2161-1009.1000351
Li, C., Zhang, Y., Tang, L., Zhao, H., Gao, C., Gao, L., … Liu, J. (2016). Expression of factors involved in the regulation of angiogenesis in the full-term human placenta: Effects of in vitro fertilization. Reproductive Biology, 16 (2), 104–112. https://doi.org/10.1016/j.repbio.2016.02.003
Choux, C., Carmignac, V., Bruno, C., Sagot, P., Vaiman, D., & Fauque, P. (2015). The placenta: phenotypic and epigenetic modifications induced by Assisted Reproductive Technologies throughout pregnancy. Clinical Epigenetics, 7 (1), 87–107. https://doi.org/10.1186/s13148-015-0120-2
Burkova, E. E., Dmitrenok, P. S., Sedykh, S. E., Buneva, V. N., Soboleva, S. E., & Nevinsky, G. A. (2014). Extremely Stable Soluble High Molecular Mass Multi-Protein Complex with DNase Activity in Human Placental Tissue. PLoS ONE, 9 (11), e111234. https://doi.org/10.1371/journal.pone.0111234
Tsyganova, N. A., Khairullin, R. M., Terentyuk, G. S., Khlebtsov, B. N., Bogatyrev, V. A., Dykman, L. A., … Khlebtsov, N. G. (2014). Penetration of Pegylated Gold Nanoparticles Through Rat Placental Barrier. Bulletin of Experimental Biology and Medicine, 157 (3), 383–385. https://doi.org/10.1007/s10517-014-2572-3
Gibson, E. J., Bell, D. L., & Powerful, S. A. (2014). Common Sexually Transmitted Infections in Adolescents. Primary Care: Clinics in Office Practice, 41 (3), 631–650. https://doi.org/10.1016/j.pop.2014.05.011
Leino, O., Kiviranta, H., Karjalainen, A. K., Kronberg-Kippilä, C., Sinkko, H., Larsen, E. H., … Tuomisto, J. T. (2013). Pollutant concentrations in placenta. Food and Chemical Toxicology, 54, 59–69. https://doi.org/10.1016/j.fct.2011.10.058
Sandin, S., Nygren, K.-G., Iliadou, A., Hultman, C. M., & Reichenberg, A. (2013). Autism and Mental Retardation Among Offspring Born After In Vitro Fertilization. JAMA, 310 (1), 75. https://doi.org/10.1001/jama.2013.7222
Selander, J., Cantor, A., Young, S. M., & Benyshek, D. C. (2013). Human Maternal Placentophagy: A Survey of Self-Reported Motivations and Experiences Associated with Placenta Consumption. Ecology of Food and Nutrition, 52 (2), 93–115. https://doi.org/10.1080/03670244.2012.719356
Vladimirov, Iu. A., Dobretcov, G. E. (1980). Fluorestcentnye zondy v issledovanii biologicheskikh membran. Moscow: Nauka, 320.
Ivanov, L. V., Orlova, I. N. (2000). Biofarmatcevticheskie issledovaniia, napravlennye na optimizatciiu sostava, svoistv i puti vvedeniia lekarstvennykh preparatov. «Tekhnologiia i standartizatciia lekarstv», 2, 558–615. Kharkiv.
Ivanov, L. V., Byzov, V. V., Sandomirskii, B. P., Galchenko, S. E. (2002). Farmakom, 2, 78–82.
Copyright (c) 2020 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).