DOI: https://doi.org/10.24959/ubphj.20.252

Study of the molecular mass fractions distribution of porcine placenta aqueous extract by the method of highly efficient gel permeation chromatography

L. Ivanov, O. Shcherbak, L. Derymedvid, V. Kravchenko, O. Bezugla

Abstract


Topicality. Placenta-based cosmetics are widely used all over the world.
Aim. To investigate the composition of water-soluble porcine placenta fractions and to study the similarity of their components to model membranes.
Materials and methods. The molecular weight distribution of the VESP fractions was studied by high-performance gel permeation chromatography. Molecular weights were determined by the retention time on the column of reference substances. To study the similarity of VESP peptides for model liposome membranes with phosphatidylcholine, the method of fluorescent probes was used.
Results and discussion. VESP contains a sufficient number (24.2 %) of short peptides and free amino acids, a large number of low molecular weight peptides of one or another molecular weight. VESP peptides effectively bind to phosphatidylcholine liposomes.
Conclusions. Due to its rich chemical composition, VESP is a promising raw material for the creation of cosmetic products.


Keywords


pork placenta aqueous extract; high performance gel permeation chromatography method; cosmetic products

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GOST Style Citations


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Abbreviated key title: Ukr. biopharm. j.

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