DOI: https://doi.org/10.24959/ubphj.20.253

The experimental study of the «Arthritan» drug influence on the induced and spontaneous pain reactions

Yu. Naboka, N. Zubitska, I. Zupanets, S. Shebeko, I. Otrishko

Abstract


Topicality. Over the past decades, traditional medicines, in particular herbal preparations, have become increasingly widespread worldwide, due to their multivector pharmacodynamics, a high enough level of safety and accessibility to a wide population.
Aim. To conduct a preclinical trials to study the analgesic properties of the drug “Arthritan” and phytocomposition based on it.
Materials and methods. The study of analgesic properties was carried out under conditions of the development of inflammatory hyperalgesia in 50 rats of both sexes weighing 150-180 g, during which the intensity of the pain reaction was determined according to the Randall-Selitto method. The influence of the studied objects on the course of spontaneous pain response in 40 rats was evaluated in the conditions of acute arthritis in the knee joint.
Results and discussion. In the study of analgesic properties in a model of induced pain response in rats with inflammatory hyperalgesia, the combination of “Arthritan”, “Nevrin” and “Nephrolik” showed a high level of analgesic activity, amounted to 30.5 %, significantly exceeded the activity of other drugs. The analgesic activity of the “Arthritan” and “Nevrin” preparations was 20.8 % and 15.9 %, respectively; the “Nephrolik” preparation did not have a significant analgesic effect. Under conditions of the development of a spontaneous pain reaction in rats with acute gonarthritis, the studied phytocomposition showed a high level of analgesic effect with repeated administrations, the activity of which  n the 5th day of the experiment was 41.9 %, which significantly exceeds all other means. The preparations “Arthritan” and “Nevrin” showed activity – 19.1 % and 17.9 %, respectively, and the drug “Nephrolic” – 7.8 %, which is an unreliable result. The high level of analgesic activity of the studied phytocomposition is due to the potentiating interaction of its components such as “Arthritan” and “Nevrin”.
Conclusions. The results of the studies allow us to recommend further research of the combined preparation containing “Arthritan”, “Nevrin” and “Nephrolik” at a ratio of 1 : 1 : 0.5 as a means of chondroprotective, anti-inflammatory and analgesic action in rheumatological desease profile patients.


Keywords


“Arthritan”; analgetic activity; induced pain; spontaneous pain

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Abbreviated key title: Ukr. biopharm. j.

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