DOI: https://doi.org/10.24959/ubphj.20.254

The choice substantiation of the concentration of water-soluble protein-polysaccharide complex of Оyster mushroom for the soft medicinal form withanti-scarring action

O. Kolpakova, N. Kucherenko, O. Hanina

Abstract


Topicality. The problem of scar formation therapy is quite urgent. It is connected with presence of large number of patients having this pathology, the frequency and unpredictable appearance of scars, which significantly impairs the person quality of life and causes a great deal of inconvenience.
Aim. To establish the optimal concentration of active substance for the soft medicinal form with anti-scarring action.
Materials and methods. Modeling of the effect of water-soluble protein-polysaccharide complex (WSPPC) on a cartilage of chicken with followed microscopic examination was performed to assess the impact of this substance effect on collagen fibers. Micropreparations were prepared and stained in accordance with well-known methods.
Results and discussion. During a microscopic examination it was found that under the influence of an active substance hyaline cartilage is destroyed due to the intercellular matrix destruction and an increase in the area of cartilage cells. Morphometric evaluation of the results showed that the introduction of 5 % WSPPC in the composition of the soft dosage form will provide the desired degree of lysis of the connective tissue and the corresponding pharmacological effect of the medicine.
Conclusions. During the study the optimal concentration of the active substance was established, which is sufficient for the manifestation of influence on the connective tissue and in the future will provide an anti-scarring effect of the created medicine.


Keywords


concentration of the active substance; ointment; water-soluble protein-polysaccharide complex; microscopy

References


Zhukov, K. A., Romanenko, Y. M., Radionov, D. V. (2014). Ukraїnskii medichnii almanakh, 4, 136–139.

Voronkov, A. V., Stepanova, Ye. F., Zhidkova, Yu. Yu. (2013). Fundamentalnye issledovaniia, 3, 301–308.

Varinsky, B., Kucherenko, N., & Kolpakova, O. (2018). The research of free aminoacids of water-soluble protein-polysaccharide complex of oyster mushroom pleurotus ostreatus. ScienceRise: Pharmaceutical Science, 4 (14), 32–37. https://doi.org/10.15587/2519-4852.2018.141412

Kucherenko, N. V., Martynov, A. V., Demianenko, V. H. (2008). Farmatsevtychnyi zhurnal, 1, 92–95.

Demianenko, V. H., Kucherenko, N. V., Stolyetov, Yu. V. (2006). Klinichna farmatsiia, 1, 36–39.

Pertcev, I. M., Kotenko, A. M., Chueshov, O. V., Khalaeva, E. L. (2008) Farmatcevticheskie i biologicheskie aspekty mazei. Kharkov : NUPh, 288.

Afanasev, Iu. I., Iurina, N. A., Kotovskii, E. F. (Eds.) (2012). Gistologiia, embriologiia, tcitologiia: uchebnik (6-edition). Moscow : GEOTAR-Media, 800.

Korzhevskii, D. E. (Ed.). (2013). Morfologicheskaia diagnostika. Podgotovka materiala dlia gistologicheskogo issledovaniia i elektronnoi mikroskopii: rukovodstvo. St. Petersburg : SpetcLit, 127.

Novak, V. P., Melnychenko, A. P. (2005). Tsytolohiia, histolohiia, embriolohiia : Navch. Posibnyk. Bila Tserkva, 256.


GOST Style Citations


1. Жуков, К. А. Современные методы терапии рубцов / К. А. Жуков, И. М. Романенко, Д. В. Радионов // Укр. мед. альманах. – 2014. – № 17 (4). – С. 136–139.


2. Воронков, А. В. Современные подходы фармакологической коррекции патологических рубцов / А. В. Воронков, Э. Ф. Степанова, Ю. Ю. Жидкова // Фундаментальные исследования. – 2013. – № 3. – С. 301–308.


3. Varinsky, B. The research of free aminoacids of water-soluble protein-polysaccharide complex of oyster mushroom pleurotusostreatus / B. Varinsky, N. Kucherenko, O. Kolpakova // Sci. J. “ScienceRise : Pharmaceutical Science”. – 2018. – № 4 (14). – P. 32–37. https://doi.org/10.15587/2519-4852.2018.141412


4. Кучеренко, Н. В. Розробка методик стандартизації водорозчинного білково-полісахаридного комплексу, отриманого з гриба PLEUROTUS OSTREATUS / Н. В. Кучеренко, А. В. Мартинов, В. Г. Дем’яненко// Фармац. журн. – 2008. – № 1. – С. 92–95.


5. Дем’яненко, В. Г. Гіполіпідемічна дія сухого екстракту гриба Pleurotusostreatus / В. Г. Дем’яненко, Н. В. Кучеренко, Ю. В. Столєтов // Клін. фармація. – 2006. – № 1. – С. 36–39.


6. Фармацевтические и биологические аспекты мазей / И. М. Перцев, А. М. Котенко, О. В. Чуешов, Е. Л. Халаева. – Х. : Изд-во НФаУ «Золотые
страницы», 2008. – 288 с.


7. Гистология, эмбриология, цитология : учебник / Ю. И. Афанасьев, Н. А. Юрина, Е. Ф. Котовский и др. ; под ред. Ю. И. Афанасьева, Н. А. Юриной. – 6-е изд., перераб. и доп. – М. : ГЭОТАР-Медиа, 2012. – 800 с.


8. Морфологическая диагностика. Подготовка материала для гистологического исследования и электронной микроскопии : руководство / под ред. Д. Э. Коржевского. – С.Пб. : СпецЛит, 2013. – 127 с.


9. Новак, В. П. Цитологія, гістологія, ембріологія : навч. посіб. / В. П. Новак, А. П. Мельниченко. – Біла Церква, 2005. – 256 с.





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)