DOI: https://doi.org/10.24959/ubphj.20.260

Morphological and anatomic structure of Rhododendron luteum Sweet leaves

T. Gontova, V. Gaponenko, K. Proskurina, V. Rudenko, O. Levashova

Abstract


Topicality. Rhododendrons are one of the most popular plants that are widely cultivated in most European countries as ornamental, essential oil, honey and insecticidal plants. Rhododendron luteum Sweet is a polymorphic species found in Ukraine as a wild-growing and ornamental plant. The chemical composition of yellow rhododendron is represented mainly by essential oils, flavonoids, hydroxycinnamic and organic acids, and triterpene and coumarin substances. Leaves are widely used in folk medicine as a diuretic, diaphoretic, astringent, wound healing, anti-inflammatory and analgesic. As a result of the study of the morphological and anatomical structure of the yellow rhododendron leaves, its structurally foliar characters under the conditions of this ecotype growth in Ukraine were studied according to a preliminary analysis of published data. Our studies were carried out with the aim of using macroscopic and microscopic traits of leaves of this species to standardize medicinal plant materials and develop quality control methods.
Aim. To identify the leaves of Rhododendron luteum (L.) Sweet by macro and microscopic characteristics. Set the main diagnostic signs of leaves.
Materials and methods. The objects of the study were samples of yellow rhododendron leaves collected during the mass flowering period. Microscopicstudies were performed on raw materials fixed in a mixture of  alcohol-glycerolwater (1 : 1 : 1). We used an OLYMPUS Lens FE-140 camera, an MBI-6 microscope, and a Biola-M microscope.

Results and discussion. The main morphological and anatomical signs of yellow rhododendron leaves were determined. Macroscopic features include simple short-leaved leaves with a leathery, hollow leaf blade, with a solid edge, a pointed tip, a wedge-shaped base, cirrus venation; microscopic is dorsoventrally type of leaf blade structure. The cells of the upper epidermis are large, thin-walled, sinuous, without stomata, covered with a thick layer of cutin; cells of the lower epidermis are slightly tortuous; stomatal apparatus of the paracitic type, typical of the abaxial epiderm, covering and glandular trichomes are present. Ferruginous club-shaped emergenes on a multicellular stand, the cells of which accumulate a yellowish-brown secretion, the secreting head is oval-cylindrical, multicellular, with dark contents. Covering hairs is of three varieties: 1-2-cell, long, curled, spiky prevail, straight-walled hairs with an expanded base and long, straight-walled, thin-walled hairs are less common. The cut is round-triangular in cross section, the angular collenchyma is underlying the epidermis; in parenchymal cells, frequent crystals of calcium oxalate - druses and prismatic crystals.

Conclusions. The results of macroscopic and microscopic study characteristics of yellow rhododendron leaves will be used to standardize medicinal plant materials and develop quality control methods.


Keywords


yellow rhododendron; leaves; macro- and microscopic signs of plant material

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Abbreviated key title: Ukr. biopharm. j.

ISSN 2519-8750 (Online), ISSN 2311-715X (Print)